Skip to main content

Fumigation by bacterial volatile 2, 5-dimethylpyrazine enhances anthracnose resistance and shelf life of mango


Anthracnose is a serious postharvest disease of mango causing significant loss in quality during ripening, storage, and trade. In the present study, we evaluated the preventive and curative effects of volatile organic compounds (VOCs) emitted by Pseudomonas putida BP25 and its synthetic VOC 2, 5-dimethylpyrazine on anthracnose in mango cv. Chausa. The preventive fumigation of fruits showed lower anthracnose severity (18.44%) than curative treatment (49.20%). Interestingly, the fumigation treatment significantly (p < 0.05) maintained fruit quality parameters such as firmness, total soluble solids, and titratable acidity. The volatile fumigated fruits showed higher bioactive content (total carotenoids, total phenolics, and total flavonoids content) and lower physiological activity (respiration and ethylene rate) than untreated fruits. The shelf life evaluation revealed an extension of storage periods for nine days. The non-invasive chlorophyll fluorescence technique further confirmed positive quality changes in the volatile fumigated fruits. Results show the potential of the bacterial-volatile blend and the synthetic 2, 5-dimethylpyrazine for increasing fruit resistance to anthracnose and extending the shelf life in mango.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6


Download references


We are thankful to the Post Graduate School, Division of Food Science and Postharvest Technology, and Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 for providing research facilities for conducting various experiments.

Author information

Authors and Affiliations



RG and AK designed and planned the experiment. ATJ carried out the experimental work. EV carried out the data analysis. AK and ATJ drafted the manuscript, further corrected and improved by CK, AK, and RG. SK helped with the resource. All authors have read the manuscript and approved the same for publication.

Corresponding authors

Correspondence to Aundy Kumar, Charanjit Kaur or Robin Gogoi.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Janamatti, A.T., Kumar, A., Kaur, C. et al. Fumigation by bacterial volatile 2, 5-dimethylpyrazine enhances anthracnose resistance and shelf life of mango. Eur J Plant Pathol 164, 209–227 (2022).

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Chlorophyll fluorescence
  • Pyrazine
  • Mango
  • Postharvest quality
  • Pseudomonas putida BP25
  • Volatiles