Abstract
High-throughput sequencing (HTS) of three pear trees (Pyrus communis) resulted in the identification of two isolates of a Tepovirus species and one isolate of a Robigovirus species, both genera from the family Betaflexiviridae and never reported in pears. Their complete genomes were fully characterized, revealing genomes of about 6.8 kb (Tepovirus) and 8.4 kb (Robigovirus). According to the species demarcation criteria in the family Betaflexiviridae, the tepovirus isolates reported here are divergent isolates of Prunus Virus T (PrVT). The robigovirus isolate had enough difference in pairwise comparison with its closer related species, cherry virus Turkey (CVTR) and it should be considered a new species of this genus, tentatively named as pomes virus Greece (PVGR). A survey was conducted using specific primers designed for each of the viruses and one additional pear accession tested positive to PrVT. Furthermore, both viruses were successfully graft-transmissible to Malus domestica or Malus micromalus, suggesting their potential to be transmitted to Malus species. This study expands the list of viruses infecting pome fruit trees and augments our knowledge on the molecular diversity and host range evolution of betaflexiviruses.
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Data availability
The data presented in this study are openly available in GenBank at reference numbers MW331539, MW331540, and MW331541.
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Acknowledgements
The authors thank Benjamin Atha for his help with RNA libraries construction and HTS, Dr. Vanitharani Ramachandram for her help with the RACE experiments, Mary O`Connell for her help with RNA extractions and Jing Zhou for manuscript review and suggestions.
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This research was funded by USDA APHIS project 20–8224-2121 IA.
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Conceptualization, L.C.C., O.P.H.-G.; Methodology, L.C.C., X.H., M.M.W., O.P.H.-G.; Software, L.C.C., X.H. O.P.H.-G.; Validation, L.C.C.; Formal analysis, L.C.C., X.H., O.P.H.-G.; Investigation, L.C.C., X.H., O.P.H.-G; Resources, J.F., C.M., O.P.H.-G.; Data curation, L.C.C., X.H., O.P.H.-G.; Writing original draft preparation, L.C.C.; Writing review and editing, L.C.C., X.H., M.M.W., J.F., C.M., O.P.H.-G.; Visualization, L.C.C., O.P.H.-G.; Supervision, O.P.H.-G.; Project administration, O.P.H.-G.; Funding acquisition, C.M., J.F., O.P.H.-G. All authors have read and agreed to the published version of the manuscript.
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Costa, L.C., Hu, X., Malapi-Wight, M. et al. Identification of a novel robigovirus and a Prunus-infecting tepovirus in Pyrus communis and their transmissibility on Malus spp.. Eur J Plant Pathol 162, 275–288 (2022). https://doi.org/10.1007/s10658-021-02402-9
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DOI: https://doi.org/10.1007/s10658-021-02402-9