Abstract
Detection of chitinase activity after electrophoresis in native polyacrylamide gels containing glycol chitin showed that Streptomyces macrosporeus VTCC 940003 produced several chitinases. Chito-oligosaccharides (COS) were produced from commercial chitosan using these chitinases. The degree of polymerization (DP) of the produced COS was confirmed by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Moreover, the biological activities of the COS against Botrytis cinerea in vitro and in vivo were assessed. The COS preparation contained a mixture of oligomers with DP ranging from 2 to 10. The COS also showed inhibition activities on spore germination, mycelial growth and infection of B. cinerea on tomato leaves.
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Acknowledgements
The authors wish to acknowledge the financial support of the grant “Scale-up production of chitosan oligomer from shrimp shells waste to be used in plant protection”, code QG.16.87 of Vietnam National University, Hanoi. The support from Walloniae-Brussels International through the grant “Industrialisation de la production d’un biopesticide pour la protection de cultures de légumes au Vietnam” (Project 13, period 2016–2018) was also greatly appreciated.
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the financial support: i) of the grant “Scale-up production of chitosan oligomer from shrimp shells waste to be used in plant protection”, code QG.16.87 of Vietnam National University, Hanoi; ii) Walloniae-Brussels International through the grant „ Industrialisation de la production d’un biopesticide pour la protection de cultures de légumes au Vietnam“(Project 13, period 2016–2018).
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Anh, T.T.V., Uyen, N.Q., Hop, D.V. et al. Chito-oligosaccharide production by chitinase of Streptococcus macrosporeus VTCC 940003 and their inhibition activities on Botrytis cinerea. Eur J Plant Pathol 161, 185–193 (2021). https://doi.org/10.1007/s10658-021-02313-9
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DOI: https://doi.org/10.1007/s10658-021-02313-9