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Genetic analysis of bacterial wilt resistance in eggplant (Solanum melongena L.)

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Abstract

Bacterial wilt triggered by Ralstonia solanacearum is one of the major devastating diseases causing significant yield reduction in eggplant. F1 hybrids (18 CARI-1 based F1 hybrids and 12 publicly available hybrids/varieties) were evaluated for bacterial wilt resistance, yield, and yield attributing traits. CARI-1 based F1 hybrids showed superiority in yields, however showed a varied level of resistance to bacterial wilt. Further, inheritance and gene action involved in resistance to Ralstonia solanacearum was studied. CARI-1 was crossed with two susceptible parents, Arka Neelachal Shyama and Pusa Purple Long to develop six generations for both the combinations. The genetic control of resistance to bacterial wilt was found to be polygenic in the case of the Arka Neelachal Shyama x CARI-1 derived population. While di-genic with complementary gene action in the case of Pusa Purple Long x CARI-1 F2 population, however deviation in expected ratios was observed in back cross populations. Generation mean analysis revealed the presence of epistasis. For Arka Neelachal Shyama x CARI-1 derived population, owing to the prevalence of additive × additive non-allelic interaction with a negative sign, delaying of selection to later generations, otherwise inter mating between the selected segregates accompanied by selfing for one or more generation(s) is advised for the aggregation of favorable alleles for enhancement of resistance. Similarly, for Pusa Purple Long x CARI-1 population, the simple selection can be efficient due to the prevalence of additive gene action. The inheritance details accumulated in this study would facilitate the introgression of bacterial wilt resistance into elite commercial genotypes.

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References

  • Acharya, B., Ghorai, A. K., Dutta, S., Maurya, P. K., Dutta, S., Mandal, A. K., & Hazra, P. (2018). Genetics of tolerance to bacterial wilt disease in tomato (Solanum lycopersicum L.). Australasian Plant Pathology, 47(6), 591–600.

    Article  CAS  Google Scholar 

  • Ajjappalavara, P. S., Dharmatti, P. R., Salimath, P. M., Patil, R. V., Patil, M. S., & Krishnaraj, P. U. (2008). Genetics of bacterial wilt resistance in brinjal. Karnataka. Journal of Agricultual Scence, 21, 424–427.

    Google Scholar 

  • Akira, M. N., Masao, K. S., Hideki, T., & Shigehito, T. (2009). Visualization of Ralstonia solanacearum cells during biocontrol of bacterial wilt disease in tomato with Pythium oligandrum. Journal of General Plant Pathology, 75, 281–287.

    Article  Google Scholar 

  • Bainsla, N. K., Singh, S., Singh, P. K., Kumar, K., Singh, A. K., & Gautam, R. K. (2016). Genetic behavior of bacterial wilt resistance in brinjal (Solanum melongena L.) in tropics of Andaman and Nicobar islands of India. American Journal of Plant Science, 7, 333–338.

    Article  CAS  Google Scholar 

  • Bhutia, N. D., Seth, T., Shende, V. D., Dutta, S., & Chattopadhyay, A. (2015). Estimation of heterosis, dominance effect and genetic control of fresh fruit yield, quality and leaf curl disease severity traits of chilli pepper (Capsicum annuum L.). Scientia Horticulturae, 182, 47–55.

    Article  Google Scholar 

  • Buyela, D. K., David, M. M., George, T. O., & Pascaline, J. (2017). Isolation and characterization of Ralstonia solanacearum strains of tomato wilt disease from Maseno, Kenya. Journal of Asian Scientific Research, 7(9), 404–420.

    Article  Google Scholar 

  • Campbell, C. L., & Madden, L. V. (1990). Introduction to plant disease epiodemiology. New York: John Wiley and sons.

    Google Scholar 

  • Cao, B. H., Lei, J. J., Wang, Y., & Chen, G. J. (2009). Inheritance and identification of SCAR linked to bacterial wilt-resistance in eggplant. African Journal of Biotechnology, 8, 5201–5207.

    CAS  Google Scholar 

  • Cavalli, L. L. (1952). A joint-scale test. In E. C. R. Rieve & C. H. Waddington (Eds.), An analysis of linkage in quantitative inheritance (pp. 135–144). London: HMSO.

    Google Scholar 

  • Chandrashekara, K. N., Prasannakumar, M. K., Deepa, M., Vani, A., & Khan, A. N. A. (2012). Prevalence of races and biotypes of Ralstonia solanacearum in India. Journal of Plant Protection Research, 52(1), 53–58.

    Article  Google Scholar 

  • Chattopadhyay, A., Seth, T., Dutta, S., Ghosh, P. P., Chattopadhyay, S. B., Majumder, D., & Hazra, P. (2012). Breeding eggplant for higher productivity and bacterial wilt tolerance. International journal of vegetable science, 18(4), 376–392.

    Article  Google Scholar 

  • Chaudhary, D. R. (2000). Inheritance of resistance to bacterial wilt (Ralstonia solanacearum E. F. Smith) in eggplant. Haryana Journal of Horticultural Sciences, 29, 89–90.

    Google Scholar 

  • Choudhary, D. K., Sun, N., Dar, M. S., & Khan, K. A. (2018). Ralstonia solanacearum: A wide spread and global bacterial plant wilt pathogen. Journal of Pharmacognosy and Phytochemistry, 7(2), 85–90.

    CAS  Google Scholar 

  • Daunay, M. C., & Hazra, P. (2012). In: Peter, K.V., Hazra, P. (Eds.), “Eggplant,” in Handbook of Vegetables (pp. 257–322). Houston:Studium Press.

  • de Mendiburu, F. (2014). Agricolae: Statistical procedures for agricultural research. http://CRAN.R-project.org/package=agricolae, R package version 1.2–1.

  • Denny, T. P. (2006). In S. S. Gnanamanickam (Ed.), Plant pathogenic Ralstonia species. In Plant-Associated Bacteria (pp. 573–644). Dordrecht, The Netherlands: Springer.

    Google Scholar 

  • Du, H., Chen, B., Zhang, X., Zhang, F., Miller, S. A., Rajashekara, G., & Geng, S. (2017). Evaluation of Ralstonia solanacearum infection dynamics in resistant and susceptible pepper lines using bioluminescence imaging. Plant Disease, 101(2), 272–278.

    Article  PubMed  Google Scholar 

  • FAOSTAT (2018). http://www.fao.org/faostat/en/

  • Fegan, M., & Prior, P. (2005). How complex is the ‘Ralstonia solanacearum species complex?’. In C. Allen, P. Prior, & A. C. Hayward (Eds.), Bacterial wilt disease and the Ralstonia solanacearum species complex (pp. 449–461). MN: APS press, Saint Paul.

  • Gopalakrishnan, C., Singh, T. H., & Artal, R. B. (2014). Evaluation of eggplant accessions for resistance to bacterial wilt caused by Ralstonia solanacearum (E.F. smith). Journal of Horticultural Science, 9, 202–205.

    Google Scholar 

  • Hayman, B. I. (1958). Separation of epistatic from additive and dominance variation in generation means. Heredity, 12, 371–390.

    Article  Google Scholar 

  • Hayman, B. I., & Mather, K. (1955). The description of genetic interaction in continuous variation. Biometrics, 11, 69–82.

    Article  Google Scholar 

  • Huet, G. (2014). Breeding for resistances to Ralstonia solanacearum. Frontiers in Plant Science, 5, 715.

    Article  PubMed  PubMed Central  Google Scholar 

  • Ishihara, T., Mitsuhara, I., Takahashi, H., & Nakaho, K. (2012). Transcriptome analysis of quantitative resistance-specific response upon Ralstonia solanacearum infection in tomato. PLoS One, 7, e46763.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jinks, J. L., & Jones, R. M. (1958). Estimation of components of heterosis. Genetics, 43, 223–234.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khapte, P., Singh, T. H., & Lakshmana, D. C. (2018). Screening of elite eggplant (Solanum melongena) genotypes for bacterial wilt (Ralstonia solanacearum) in field conditions and their genetic association by using SSR markers. Indian Journal of Agricultural Science, 88(10), 1502–1511.

    Google Scholar 

  • Krishna, K., Singh, P. K., Ajanta, B., Shrawan, S., Naresh, K., Singh, A. K., Singh, D. R., & Gautam, R. K. (2012). CARI eggplant-1 (IC0585684; INGR12015), an eggplant germplasm with bacterial wilt resistance. Indian Journal of Plant Genetic Resources, 25, 323–324.

    Google Scholar 

  • Kumar, R., Esakky, R., & Yadav, K. (2019). Phylotype analysis of Ralstonia Solanacearum causing bacterial wilt in eggplants in Orissa in India. International Journal of Trend in Scientific Research and Development, 3(3), 1–3.

    CAS  Google Scholar 

  • Kurhade, G. M., Bhave, S. G., Sawardekar, S. V., Burondkar, M. M., & Dalvi, V. V. (2017). Genetic behavior of bacterial wilt resistance in f2 population of brinjal (Solanum melongena L.). Journal of Research ANGRAU, 45(2), 1–5.

    Google Scholar 

  • Lebeau, A., Gouy, M., Daunay, M. C., Wicker, E., Chiroleu, F., Prior, P., Frary, A., & Dintinger, J. (2013). Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant. Theoretical and Applied Genetics, 126, 143–158.

    Article  CAS  PubMed  Google Scholar 

  • Mak, G. (2013). Health benefits of Aubergine. Retrieved from. www.healthbenefitstimes.com. Accessed 22 Oct 2015

  • Mansfield, J., Genin, S., Magori, S., Citovsky, V., Sriariyanum, M., Ronald, P., Dow, M., Verdier, V., Beer, S. V., & Machado, M. A. (2012). Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology, 13, 614–629.

    Article  PubMed  PubMed Central  Google Scholar 

  • Mather, K. (1949). Biometrical genetics. New York: Dover Publications Inc..

    Google Scholar 

  • Mather, K., & Jinks, J. L. (1982). Biometrical genetics, 3rd edn. Chapman and Hall Ltd., London.

  • Mensah, C., DiFonzo, C., Nelson, R. L., & Wang, D. (2005). Resistance to soybean aphid in early maturing soybean germplasm. Crop Science, 45(6), 2228–2233.

    Article  Google Scholar 

  • Murray, M. T. (2004). The healing power of herbs, 2nd edn. New York: Random House

  • Namisy, A., Chen, J., Prohen, J., Metwally, E., Elmahrouk, M., & Rakha, M. (2019). Screening of cultivated eggplant and wild relatives for resistance to bacterial wilt (Ralstonia solanacearum). Agriculture, 9(7), 157.

    Article  CAS  Google Scholar 

  • Nishat, S., Hamim, I., & Ibrahim, K. M. (2015). Genetic diversity of the bacterial wilt pathogen Ralstonia solanacearum using a RAPD marker. Comptes Rendus Biologies, 338, 757–767.

    Article  PubMed  Google Scholar 

  • Pandiyaraj, P., Singh, T. H., Reddy, K. M., Sadashiva, A. T., Gopalakrishnan, C., Reddy, A. C., Pattanaik, A., & Reddy, A. D. C. L. (2019). Molecular markers linked to bacterial wilt (Ralstonia solanacearum) resistance gene loci in eggplant (Solanum melongena L.). Crop Protection, p. 124.

  • Panse, V. G. & Sukhatme, R. V. (1985) Statistical methods for agricultural workers (4th edn, pp. 58–62). New Delhi: P. & I. Division, ICAR.

  • Parihar, A. K., Dixit, G. P., & Singh, D. (2016). Gene interactions and genetics for yield and its attributes in grass pea (Lathyrus sativus L.). Journal of Genetics, 95(4), 947–956.

    Article  CAS  PubMed  Google Scholar 

  • Prakash, N. R., Zunjare, R. U., Muthusamy, V., Chand, G., Kamboj, M. C., Bhat, J. S., & Hossain, F. (2019). Genetic analysis of prolificacy in “Sikkim primitive”—A prolific maize (Zea mays) landrace of north-eastern Himalaya. Plant Breeding, 1, 1–9.

    Google Scholar 

  • R Development Core Team (2012). R: a language and environment for statistical computing. R foundation for statistical computing. Vienna, Austria. http://www.R-project.org/

  • Rai, A. K., Sadashiva, A. T., Basavaraj, Y. B., Venugopalan, R., Rao, E. S., & Nandeesha, P. (2020). Genetic analysis of bud necrosis disease caused by groundnut bud necrosis virus (GBNV) in tomato (Solanum lycopersicum L.). Euphytica, 216(8), 1–10.

    Article  CAS  Google Scholar 

  • Ramesh, R. (2008). Bacterial wilt in brinjal and its management. Technical bulletin no: 10, ICAR research complex for Goa (Indian Council of Agricultural Research), Ela, Old Goa- 403 402, Goa, India. http://krishi.icar.gov.in/jspui/bitstream/123456789/24432/1/TB%20No.10.pdf

  • Roy, A., Acharya, S., Das, S., Ghosh, R., Paul, S., Srivastava, R. K., & Ghosh, S. K. (2009). Distribution, epidemiology and molecular variability of the begomovirus complexes associated with yellow vein mosaic disease of mesta in India. Virus Research, 141, 237–246.

    Article  CAS  PubMed  Google Scholar 

  • Saddler, G. S. (2005). Management of bacterial wilt disease. In C. Allen, P. Prior, & A. C. Hayward (Eds.), Bacterial wilt disease and the Ralstonia solanacearum species complex (pp. 121–132). St. Paul: American Phytopathological Society, APS Press.

  • Sarkar, S., & Chaudhuri, S. (2016). Bacterial wilt and its management. Current Science, 25, 1439–1445.

    Google Scholar 

  • Satyaprakash, B., Reddy, A. C., Naresh, P., Meenu, K., DC, L. R., Petikam, S., & Gs, S. (2020). Breeding for bacterial wilt resistance in eggplant (Solanum melongena L.): Progress and prospects. Crop Protection, 105270.

  • Sekara, A., Cebula, S., & Kunicki, E. (2007). Cultivated eggplants – Origin, breeding objectives and genetic resources, A review. Folia Horticulture, 19(1), 97–114.

    Google Scholar 

  • Sheoran, O. P., Tonk, D. S., Kaushik, L. S., Hasija, R. C., & Pannu, R. S. (1998). Statistical software package for agricultural research workers. In D. S. Hooda & R. C. Hasija (Eds.), Recent advances in information theory (pp. 139–143). CCS-HAU, Hisar: Statistics & Computer Applications. Department of Mathematics and Statistics.

    Google Scholar 

  • Singh, A. K., Pan, R. S., & Bhavana, P. (2014). Genetic studies for quantitative traits and resistance to bacterial wilt in brinjal (Solanum melongena). International Journal of Innovative Horticulture, 3(2), 154–157.

    Google Scholar 

  • Singh, N., Phukan, T., Sharma, P., Kabyashree, K., Barman, A., Kumar, R., Sonti, R. V., Genin, S., & Ray, S. K. (2018). An innovative root inoculation method to study Ralstonia solanacearum pathogenicity in tomato seedlings. Phytopathology, 108(4), 436–442.

    Article  CAS  PubMed  Google Scholar 

  • Tahat, M. M., & Sijam, K. (2010). Ralstonia solanacearum: The bacterial wilt causal agent. Asian Journal of Plant Science, 9(7), 385–393.

    Article  Google Scholar 

  • Thakur, A. K., Kohli, U. K., & Kumar, M. (2004). Inheritance of resistance to bacterial wilt in tomato. Indian Journal of Genetics, 64, 79–80.

    Google Scholar 

  • Uttamrao, C, P. (2012). Tagging of bacterial wilt resistance gene in Solanum melongena var. insanum by molecular markers. M.Sc. Thesis. http://krishikosh.egranth.ac.in/ha ndle/1/5810033405.

  • VanElsas, J. D., Kastelein, P., VanBekkum, P., VandesWolf, J. M., DeVries, P. M., & VanOverbeck, L. S. (2000). Survival of Ralstonia solanacearum biovar 2, the causative agent of potato brown rot, in field and microcosm soils in temperate climates. Phytopathology, 90, 1358–1366.

    Article  CAS  Google Scholar 

  • Vasse, J., Frey, P., & Trigalet, A. (1995). Microscopic studies of intercellular infection and protoxylem invasion of tomato roots by Pseudomonas solanacearum. Molecular Plant-Microbe Interactions Journal, 8, 241–251.

    Article  CAS  Google Scholar 

  • Wicker, E., Grassart, L., Coranson-Beaudu, R., Mian, D., Guilbaud, C., & Fegan, M. (2007). Ralstonia solanacearum strains from Martinique (French West Indies) exhibiting a new pathogenic potential. Applied and Environmental Microbiology, 71, 6790–6801.

    Article  CAS  Google Scholar 

  • Xi’ou, X., Bihao, C., Guannan, L., Jianjun, L., Qinghua, C., Jin, J., & Yujing, C. (2015). Functional characterization of a putative bacterial wilt resistance gene (RE-bw) in eggplant. Plant Molecular Biology Report, 33(4), 1058–1073.

    Article  CAS  Google Scholar 

  • Yuliar, Y., Nion, A., & Toyota, K. (2015). Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes and Environment, 30(1), 1–11.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Vegetable science, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, 751003, India

    Satyaprakash Barik & Sahu Gouri Shankar

  2. Central Horticultural Experiment Station (ICAR-IIHR), Aiginia, Bhubaneswar, Odisha, 751019, India

    Naresh Ponnam, Acharya G.C, Sandeep V, Meenu Kumari & Srinivas P

  3. Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, India

    Singh T.H

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  1. Satyaprakash Barik
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Barik, S., Ponnam, N., G.C, A. et al. Genetic analysis of bacterial wilt resistance in eggplant (Solanum melongena L.). Eur J Plant Pathol 160, 349–364 (2021). https://doi.org/10.1007/s10658-021-02248-1

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