Survival of Curtobacterium flaccumfaciens pv. flaccumfaciens in the phyllosphere and rhizosphere of crops

Abstract

Knowledge of the ecological survival niches of Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), the causal agent of bean bacterial wilt, is essential for the efficient disease management. Our study evaluated the survival of Cff in the phyllospheres and rhizospheres of barley, black oat, canola, common bean, forage turnip, maize, pearl millet, ryegrass, sorghum, soybean, sunflower, velvet bean, wheat and white oat planted in a greenhouse. The aerial parts of the plants were inoculated by spraying a bacterial suspension (107 CFU.mL−1) from the strain Feij. 2628A, which is resistant to rifampicin and pathogenic to common bean. The soil of the pots was infested with 200 mL of the same suspension. Cff survival was evaluated every seven days for 70 days, and the survival periods were confirmed by selecting strains from all samples and performing PCR with specific primers. Cff survived for at least seven days in the phyllosphere, and 21 days in the rhizosphere of all evaluated crops. Based on our results, barley, black oat, canola, forage turnip, maize, pearl millet, ryegrass, sorghum, soybean, sunflower, velvet bean, wheat and white oat can be potential asymptomatic hosts for Cff, and their cultivation in succession with common bean is not recommended in areas with a history of bacterial wilt occurrence.

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Acknowledgments

To Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES), for the financial support for Daniele M. Nascimento PhD thesis.

Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. Financial support was received from FAPESP 16/20737–6 and CNPq 401142/2016–7.

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Correspondence to Daniele M. Nascimento.

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Authors declared that this manuscript has not published elsewhere. All authors read and approved the final version of this manuscript. The authors declare that the present work was developed without any potential conflict of interest, with no human or animal participants.

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Nascimento, D.M., Oliveira, L.R., Melo, L.L. et al. Survival of Curtobacterium flaccumfaciens pv. flaccumfaciens in the phyllosphere and rhizosphere of crops. Eur J Plant Pathol 160, 161–172 (2021). https://doi.org/10.1007/s10658-021-02232-9

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Keywords

  • Alternative host
  • Bacterial wilt
  • Common bean
  • Crop rotation
  • Disease management
  • Ecology of phytobacteria