Skip to main content
SpringerLink
Log in

Epitypification of Cercospora coffeicola and its involvement with two different symptoms on coffee leaves in Brazil

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Cercospora coffeicola is the causal agent of brown eye spot, an important disease of coffee (Coffea arabica) in Brazil. However, atypical symptom as darker and larger lesions, named black spot, has been reported in field. In this study, we tested the hypothesis that the causal agent of black spot belongs to the same species pathogenic to brown eye spot. Nineteen strains obtained from diseased coffee found in the five largest coffee-producing states of Brazil were characterized by a combination of molecular phylogenic methods, using a multi-locus approach (internal transcribed spacer regions and intervening 5.8S nrRNAs, actin, calmodulin, histone H3, and translation elongation factor 1-alpha), analyses of morphological markers and pathogenicity. Strains from brown eye spot and black spot disease on coffee leaves formed a clade with C. coffeicola strain from Japan. All strains showed same morphological characteristics and caused brown eye spot symptoms in greenhouse. These results confirm that the species associated with brown eye spot and black spot disease on coffee leaves is C. coffeicola. Additionally, an epitype is proposed for C. coffeicola.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ariyawansa, H. A., Hawksworth, D. L., Hyde, K. D., Jones, E. B. G., Maharachchikumbura, S. S. N., Manamgoda, D. S., Thambugala, K. M., Udayanga, D., Camporesi, E., Daranagama, A., Jayawardena, R., Liu, J. K., McKenzie, E. H. C., Phookamsak, R., Senanayake, I. C., Shivas, R. G., Tian, Q., & Xu, J. C. (2014). Epitypification and neotypification: Guidelines with appropriate and inappropriate examples. Fungal Diversity, 69, 57–91.

    Article  Google Scholar 

  • Bakhshi, M., Arzanlou, M., Babai-ahari, A., et al. (2015). Application of the consolidated species concept to Cercospora spp. from Iran. Persoonia, 34, 65–86.

    Article  CAS  PubMed  Google Scholar 

  • Bakhshi, M., Arzanlou, M., Babai-ahari, A., Groenewald, J. Z., & Crous, P. W. (2018). Novel primers improve species delimitation in Cercospora. IMA Fungus, 9, 299–332.

    Article  PubMed  PubMed Central  Google Scholar 

  • Bradshaw, M., & Tobin, P. (2020). Sequencing herbarium specimens of a common detrimental plant pathogen (powdery mildew) phytopathology. First Look., 110, 1248–1254. https://doi.org/10.1094/PHYTO-04-20-0139-PER.

    Article  CAS  Google Scholar 

  • Carbone, I., & Kohn, L. M. (1999). A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia, 91, 553–556.

    Article  CAS  Google Scholar 

  • Chupp, C. (1954). A monograph of the fungus genus Cercospora. New York: Ithaca.

    Google Scholar 

  • Cooke, M. C. (1881). Some exotic fungi. Grevillea, 9(51), 97–101.

    Google Scholar 

  • Crous, P. W., & Braun, U. (2003). Mycosphaerella and its anamorphs: 1. Names published in Cercospora and Passalora. CBS Biodiversity Series, 1, 1–571.

    Google Scholar 

  • Crous, P. W., Groenewald, J. Z., Pongpanich, K., et al. (2004). Cryptic speciation and host specificity among Mycosphaerella spp. occurring on Australian Acacia species grown as exotics in the tropics. Studies in Mycology, 50, 457–469.

    Google Scholar 

  • Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). ModelTest 2: More models, new heuristics and highperformance computing. Nature Methods, 9, 772. https://doi.org/10.1038/nmeth.2109.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dell’ Acqua, R., Mantovani, E. S., Braghini, M. T., et al. (2011). Variabilidade in vitro, in vivo e molecular de isolados de Cercospora coffeicola. Tropical Plant Pathology, 36, 313–326.

    Google Scholar 

  • Ellis, M. B. (1971). Dematiaceous hyphomycetes. Kew, England: Commonwealth Mycological Institute.

    Google Scholar 

  • Fazuoli, L. C., Medina, F. H. P., Gonçalves, W., et al. (2002). Melhoramento do cafeeiro: variedades tipo arábica obtidas no Instituto Agronômico de Campinas. In L. Zambolim (Ed.), O estado da arte de tecnologias na produção de café (pp. 163–215). Brazil: Universidade Federal de Viçosa.

    Google Scholar 

  • Feau, N., Hamelin, R. C., & Bernier, L. (2006). Attributes and congruence of three molecular data sets: Inferring phylogenies among Septoria-related species from woody perennial plants. Molecular Phylogenetics and Evolution, 40, 808–829.

    Article  CAS  PubMed  Google Scholar 

  • Godoy, C. V., Bergamin-Filho, A., & Salgado, C. L. (1997). Doenças do cafeeiro. In H. Kimati, L. Amorim, A. F. Bergamin, L. E. A. Camargo, & J. A. M. Rezende (Eds.), Manual de Fitopatologia, 3rd edn (Vol. 2, pp. 184–200). Brasil: Editora Agronômica Ceres.

    Google Scholar 

  • Groenewald, J. Z., Nakashima, C., Nishikawa, J., Shin, H. D., Park, J. H., Jama, A. N., Groenewald, M., Braun, U., & Crous, P. W. (2013). Species concepts in Cercospora: Spotting the weeds among the roses. Studies in Mycology, 75, 115–170.

    Article  CAS  PubMed  Google Scholar 

  • Guatimosim, E., Schwartsburd, P. B., Barreto, R. W., et al. (2017). Novel fungi from an ancient niche: Cercosporoid and related sexual morphs on ferns. Persoonia, 37, 106–141.

    Article  Google Scholar 

  • Hyde, K. D., & Zhang, Y. (2008). Epitypification: Should we epitypify? Journal of Zhejiang University. Science. B, 9, 842–846.

    Article  PubMed  PubMed Central  Google Scholar 

  • Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lima, L. M., Pozza, E. A., & Santos, F. S. (2012). Relationship between incidence of brown eyespot of coffee cherries and the chemical composition of coffee beans. Journal of Phytopathology, 160, 209–211.

    Article  Google Scholar 

  • Nelson, S. C. (2008). Cercospora leaf spot and berry blotch of coffee. CTAHR, Univ. of Hawai, http://www.ctahr.hawaii.edu/oc/freepubs/pdf/PD-41. Accessed 16 Dec 2019.

  • Nguanhom, J., Cheewangkoon, R., Groenewald, J. Z., et al. (2015). Taxonomy and phylogeny of Cercospora spp. from northern Thailand. Phytotaxa, 233, 27–48.

    Article  Google Scholar 

  • Noack, F. (1901). Die Krankheiten des Kaffeebaumes in Brasilien. Z Pflkrankh Pflschutz, 11, 196–203.

    Google Scholar 

  • Pozza, E. A., Carvalho, L. V., & Chalfoun, S. M. (2010). Sintomas e injúrias causadas por doenças em cafeeiro. In R. J. Guimarães, A. N. G. Mendes, & D. P. Baliza (Eds.), Semiologia do cafeeiro: sintomas de desordens nutricionais, fitossanitárias e fisiológicas (pp. 68–106). Brazil: UFLA.

    Google Scholar 

  • Ronquist, F., Teslenko, M., Vander-Mark, P., et al. (2012). MrBayes3.2: Efficieznt Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    Article  PubMed  PubMed Central  Google Scholar 

  • Santos, L. A., Souza, P. E., Pozza, E. A., et al. (2014). Nova técnica para isolar Cercospora coffeicola Berkeley & Cooke, agente etiológico da cercosporiose do cafeeiro. Coffee Science, 9, 142–144.

    Google Scholar 

  • Souza, A. G. C., Rodrigues, F. A., Maffia, L. A., & Mizubuti, E. S. G. (2011). Infection process of Cercospora coffeicola on coffee leaf. Journal of Phytopathology, 159, 6–11.

    Article  Google Scholar 

  • Souza, A. G. C., Maffia, L. A., & Mizubuti, E. S. G. (2012). Cultural and aggressiveness variability of Cercospora coffeicola. Journal of Phytopathology, 160, 540–546.

    Article  Google Scholar 

  • Souza, A. G. C., Maffia, L. A., Silva, F. F., Mizubuti, E. S. G., & Teixeira, H. (2015). A time series analysis of brown eye spot progress in conventional and organic coffee production systems. Plant Pathology, 64, 157–166.

    Article  Google Scholar 

  • Walker, J. C. (1925). Studies on disease resistance in the onion. Proceedings of the National Academy of Sciences, 11, 183–189.

    Article  CAS  Google Scholar 

  • White, T. J., Bruns, T., Lee, S., et al. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). New York: Academic Press.

    Google Scholar 

Download references

Acknowledgments

This research was supported by the National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), Foundation for Research Support of the State of Minas Gerais (FAPEMIG), and the National Institute of Science and Technology of Coffee (INCT-Café).

Author information

Authors and Affiliations

  1. Faculty of Presidente Antônio Carlos of Teófilo Otoni, Teófilo Otoni, MG, 39803-087, Brazil

    Paula Adrielly Souza Vale

  2. Department of Plant Pathology, Federal University of Lavras, UFLA, Lavras, MG, 37200-000, Brazil

    Mário Lúcio Vilela de Resende, Deila Magna dos Santos Botelho, Eduardo Alves, Sarah da Silva Costa Guimarães & Ludwig H. Pfenning

  3. Agronomica, Av Ipiranga, 7464, conj.1202, Porto Alegre, RS, 90230-100, Brazil

    Camila Cristina Lage de Andrade

  4. Santa Catarina State Agricultural Research and Rural Extension Agency, Epagri, Caçador, SC, 88034-901, Brazil

    Cláudio Ogoshi

Authors
  1. Paula Adrielly Souza Vale
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mário Lúcio Vilela de Resende
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deila Magna dos Santos Botelho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Camila Cristina Lage de Andrade
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eduardo Alves
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cláudio Ogoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sarah da Silva Costa Guimarães
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ludwig H. Pfenning
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sarah da Silva Costa Guimarães.

Ethics declarations

Ethical approval

The authors declare that have followed the guidelines of the Ethical Standards requested by EJPP, and this manuscript is original and not published elsewhere.

Conflict of interest

The authors declare no conflict of interest.

Human and animal rights and informed consent

No human participants and/or animals are involved in this research.

Supplementary Information

ESM 1

(DOCX 15 kb)

Fig. S1

Maximum parsimony tree inferred from ITS sequences showing relationships among Cercospora species. Bootstrap values are presented above the nodes. Tree scores, confidence interval [CI] = 0.846 and retention index [RI] = 0.789. (PDF 92 kb)

Fig. S2

Maximum parsimony tree inferred from actA sequences showing relationships among Cercospora species. Bootstrap values are presented above the nodes. Tree scores, confidence interval [CI] = 0.948 and retention index [RI] = 0.925. (PDF 91 kb)

Fig. S3

Maximum parsimony tree inferred from his3sequences showing relationships among Cercospora species. Bootstrap values are presented above the nodes. Tree scores, confidence interval [CI] = 1.0 and retention index [RI] = 1.0. (PDF 91 kb)

Fig. S4

Maximum parsimony tree inferred from cmdA sequences showing relationships among Cercospora species. Bootstrap values are presented above the nodes. Tree scores, confidence interval [CI] = 0.924 and retention index [RI] = 0.827. (PDF 88 kb)

Fig. S5

Maximum parsimony tree inferred from tef1 sequences showing relationships among Cercospora species. Bootstrap values are presented above the nodes. Tree scores, confidence interval [CI] = 0.921 and retention index [RI] = 0.76. (PDF 85 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vale, P.A.S., de Resende, M.L.V., dos Santos Botelho, D.M. et al. Epitypification of Cercospora coffeicola and its involvement with two different symptoms on coffee leaves in Brazil. Eur J Plant Pathol 159, 399–408 (2021). https://doi.org/10.1007/s10658-020-02170-y

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-020-02170-y

Keywords

Search

Navigation