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Complete genome sequence of a distinct isolate of cassava common mosaic virus (CsCMV) infecting cassava in Hainan, China

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Abstract

The complete genomic sequence of cassava common mosaic virus isolate Linggao (CsCMV-LG) was determined from cassava (Manihot esculenta Crantz) with mild foliar mosaic symptom in Hainan Province, China. Excluding the poly(A) tail, the CsCMV-LG genome (GenBank accession No. MT038420) is 6374 nucleotides (nts) in length, with five major open reading frames encoding a 1450-amino acids (aa) RNA-dependent RNA polymerase (RdRp), three triple gene block (TGB) proteins (231-aa, 110-aa and 95-aa), and a 229-aa coat protein (CP). Phylogenetic analysis based on the complete sequence indicated that CsCMV-LG is closely related to CsCMV-Brazilian which belongs to the genus Potexvirus, but the sequence identity shared only 88.0%. Notablely, the CsCMV-LG can also infect tobacco (Nicotiana benthamiana) in laboratory through rub inoculation causing chlorosis and malformation symptoms 15 days post inoculation (dpi). The study extends genome sequence and genetic diversity of CsCMV and provides an important basis for the virus detection in cassava.

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Acknowledgements

This work was funded by the National Key R&D Program of China (2019YFD1000500), National Natural Science Foundation of China (31261140363) and Young Elite Scientists Sponsorship Program by CSTC (Project No. CSTC-QN201704).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Nai-tong Yu, Jun-hua Li, Wei-li Li & Zhi-xin Liu

  2. Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, 571101, Haikou, China

    Nai-tong Yu & Zhi-xin Liu

  3. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, 571101, Haikou, China

    Yi Yang

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  1. Nai-tong Yu
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  2. Yi Yang
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  3. Jun-hua Li
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  4. Wei-li Li
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  5. Zhi-xin Liu
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Correspondence to Nai-tong Yu or Zhi-xin Liu.

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Yu, Nt., Yang, Y., Li, Jh. et al. Complete genome sequence of a distinct isolate of cassava common mosaic virus (CsCMV) infecting cassava in Hainan, China. Eur J Plant Pathol 158, 583–587 (2020). https://doi.org/10.1007/s10658-020-02088-5

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  • DOI: https://doi.org/10.1007/s10658-020-02088-5

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