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Occurrence of the genus Dolichodorus Cobb 1914 (Nematoda: Dolichodoridae) in Iran, and description of D. rex n. sp.

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The genus Dolichodorus was discovered from a natural forest in Gilan province, north Iran, in association with an oak tree. The recovered population represents a new species, and was described and illustrated based upon morphological and molecular data, as D. rex n. sp. It is characterized by 2171–3275 μm long females having offset crown-like cephalic region with well sclerotized cephalic framework, raised perioral disc apparently with a small raised oral plate, 118.5, 130.0 μm long stylet, its conus ca. 55% of the total length and three posteriorly sloping knobs, 291–316 μm long pharynx with a short overlap, secretory-excretory pore at slightly variable position (one-two annuli posterior, or four annuli anterior to metacorpus base), vulva sunken in body, at %50 of the body length, vagina heavily sclerotized with two drop-like refractive pieces close to the vulval opening, 78–104 μm long tail with proximal convex conoid, and conical distal part with pointed to blunt tip; and a 2634 μm long male with 108.5 μm long stylet, 291 μm long pharynx, 20 μm long tail and 53 μm long spicules. In molecular phylogenetic analyses using partial sequences of the small, and D2-D3 expansion segments of the large subunit ribosomal DNA, the new species formed a clade with two other currently sequenced species of the genus in both phylogenies.

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  1. The species epithet is the Latin translation of king, referring to the specific cephalic region shape of the new species, resembling a crown.


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The studied soil samples in present study were kindly provided by Dr. Reyhaneh Gholami. This help is appreciated. We thank Dr. Hossein Shafizadeh Moghaddam for his technical helps, and Tarbiat Modares University and Iranian National Science foundation (INSF) for financial supports.

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Correspondence to Majid Pedram.

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All the authors certify that they do not have any conflict of interest, the presented data are original and the presented data are not published, and are not under evaluation for publication elsewhere, all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected. They also certify that the final version of this manuscript has been reviewed by all authors.

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The soil samples analyzed in this study were collected from the natural public regions that are not under protection against soil samplings.

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The authors certify that they have accepted the principles of ethical standards and that guidelines of professional conduct have been followed. The funders had no role in designing of this study, collecting and analyzing of the data, preparing the manuscript and are not involved in evaluating or publishing process of this research.

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Fadakar, S., Jahanshahi Afshar, F. & Pedram, M. Occurrence of the genus Dolichodorus Cobb 1914 (Nematoda: Dolichodoridae) in Iran, and description of D. rex n. sp.. Eur J Plant Pathol 158, 443–455 (2020).

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