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Molecular, morphological and pathogenic characterization of Clavibacter michiganensis subsp. sepedonicus strains of different geographic origins in Georgia

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Abstract

Potato ring rot and its causative pathogen Clavibacter michiganensis subsp. sepedonicus (Cms) have been studied in Georgia for the first time. The samples were collected in the fields and potato storage facilities in various potato producing regions of Georgia. Potato tubers were also collected at agrarian markets and the border checkpoints, from which potatoes are imported from neighboring countries. In 124 potato samples the detection of Cms was performed using specific PCR analysis and bioassay test. A total of 20 Cms isolates were purified and identified by subspecies-specific PSA-1 and PSA-R primers set. The isolates were characterized according to cell morphology, cultural-biochemical properties and virulence. The study of genetic diversity using molecular typing methods of PCR melting profile with different restriction enzymes and variable number tandem repeats PCR revealed significant differences between Cms isolates, which is not related to their geographical origin.

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Acknowledgements

The Study was supported by the Regional Cooperative Research Grant project #60958, funded by the U.S. Defense Threat Reduction Agency through CRDF Global/GRDF.

We thank the State Laboratory of Agriculture of Georgia for kindly provided potato samples from Georgia’s border checkpoints.

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Authors and Affiliations

  1. Durmishidze Institute of Biochemistry and Biotechnology, Agricultural University of Georgia, 240 David Aghmashenebeli Alley, 0159, Tbilisi, Georgia

    Tinatin Sadunishvili, Dali Gaganidze, Nanuli Amashukeli, Neli Sturua, Lia Amiranashvili, Shorena Kharadze & Giorgi Kvesitadze

  2. Plant Breeding and Acclimatization Institute - National Research Institute, Radzików, 05-870, Błonie, Poland

    Agnieszka Węgierek-Maciejewska & Edward Arseniuk

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  1. Tinatin Sadunishvili
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  2. Agnieszka Węgierek-Maciejewska
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  9. Giorgi Kvesitadze
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Correspondence to Tinatin Sadunishvili.

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Authors declare that the manuscript is entirely original and have not been, and will not be, published elsewhere if accepted for publishing in EJPP. The content and authorship of the submitted manuscript has been approved by all authors, and all prevailing local, national and international regulations and conventions, and normal scientific ethical practices have been respected. Authors declare no competing interests.

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The authors TS, A.W-M., EA, DG, NA, NS, LA, SK and GK declare that they have no conflict of interest. Each authors provided the corresponding author with signed potential conflict of interest disclosure form.

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Sadunishvili, T., Węgierek-Maciejewska, A., Arseniuk, E. et al. Molecular, morphological and pathogenic characterization of Clavibacter michiganensis subsp. sepedonicus strains of different geographic origins in Georgia. Eur J Plant Pathol 158, 195–209 (2020). https://doi.org/10.1007/s10658-020-02066-x

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  • DOI: https://doi.org/10.1007/s10658-020-02066-x

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