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Nematicidal activity of flavonoids with structure activity relationship (SAR) studies against root knot nematode Meloidogyne incognita

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Abstract

In continuation of our work on Tagetes spp., the current study deals with the nematicidal activity of flavonoids, which are structurally related to two marker compounds, patuletin and patulitrin isolated from Tagetes patula flowers. The activity was determined against root-knot nematode, Meloidogyne incognita to establish structure activity relationship. In vitro examination of flavonoids belonging to flavone and flavonol classes revealed significant differences in nematicidal ability depending on their structural motif, concentration, and time of exposure. In case of flavonols the most efficient compounds were found to be fisetin, galangin, isorhamnetin, morin, and myricetin which exhibited 100% mortality of 2nd stage juveniles after 24 h of incubation. Whereas, among flavones which showed the same excellent activity parameters, were simple flavone, 6-hydroxy flavone, chrysin, 6-methoxy apigenin, apigenin-7-O-glucoside and 6-methoxy luteolin. These results revealed that flavonoids possess promising nematicidal activity and may have potential use in crop management as active agents.

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Acknowledgements

Dr. Sadia Zik-ur-Rehman and Dr. Samina Bano were financially supported by Higher Education Commission Islamabad through Start-up Research Grant Program (SRGP) for the project entitled “Tagetes spp. A rich source of nematicidal agents” under Interim Placement of Fresh Ph.D’s (IPFP) program, and “Indigenous 5000 Fellowship Program (Batch-V)”, respectively.

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Authors and Affiliations

  1. National Nematological Research Centre, (NNRC), University of Karachi, Karachi, Pakistan

    Samina Bano, Erum Yawar Iqbal & Shahina Fayyaz

  2. HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan

    Lubna, Sadia Zik-ur-Rehman & Shaheen Faizi

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  1. Samina Bano
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  2. Erum Yawar Iqbal
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  3. Lubna
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  4. Sadia Zik-ur-Rehman
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  5. Shahina Fayyaz
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  6. Shaheen Faizi
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Correspondence to Samina Bano or Shahina Fayyaz.

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This research does not contain any conflicts of interest, nor research involving humans or animals.

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In memory of Prof. Dr. Salimuzzman Siddiqui FRS on his one hundred and twenty-third (123rd) birthday (October 19, 1897- April 14, 1994); he was the founding director of H.E.J Research Institute of Chemistry, University of Karachi.

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Bano, S., Iqbal, E.Y., Lubna et al. Nematicidal activity of flavonoids with structure activity relationship (SAR) studies against root knot nematode Meloidogyne incognita. Eur J Plant Pathol 157, 299–309 (2020). https://doi.org/10.1007/s10658-020-01988-w

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