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Specific genes and sequence variation in pathotype 7 of the clubroot pathogen Plasmodiophora brassicae

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Abstract

Plasmodiophora brassicae is a biotrophic pathogen and causes clubroot of cruciferous crops. Our study was conducted to find genetic markers for differentiating the pathotype 7. Three molecular markers were obtained, PBRA_000303 presented exclusively in pathotype 7 among the tested pathotypes (P2, P4, P5, P7, P9, P10, P11), PBRA_006533 and PBRA_009559 presented in pathotype 7 and a genotype of pathotype 4 (P4*). The similar results were obtained by clustering analysis based on SRAP and the small subunit (SSU) of P. brassicae rDNA sequence: P7 and P4* were clustered a clade, most of P4 was clustered into another clade. A 380 bp fragment in SSU was existent in P7 and P4* but scarce in most P4. Virulence analysis discovered that pathotype 7 had similar pathogenicity to P4* but lower pathogenicity than most pathotype 4. Although the genetic information had similarity among different pathotype, it is noteworthy that this study is the first to establish a specific molecular identification system for pathotype 7 by PCR analysis, and provides a promising tool to conveniently monitor the distribution of P7.

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References

  • Chai, A. L., Xie, X. W., Shi, Y. X., & Li, B. J. (2014). Research status of clubroot (Plasmodiophora brassicae) on cruciferous crops in China. Canadian Journal of Plant Pathology, 36, 142–153.

    Article  Google Scholar 

  • Diederichsen, E., Frauen, M., Linders, E. G. A., Hatakeyama, K., & Hirai, M. (2009). Status and perspectives of clubroot resistance breeding in crucifer crops. Journal of Plant Growth Regulation, 28, 265–281.

    Article  CAS  Google Scholar 

  • Dixon, G. R. (2009). The occurrence and economic impact of Plasmodiophora brassicae and clubroot disease. Journal of Plant Growth Regulation, 28, 194–202.

    Article  CAS  Google Scholar 

  • Donald, C., & Porter, I. (2009). Integrated control of clubroot. Plant Growth Regulation, 28, 289–303.

    Article  CAS  Google Scholar 

  • Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791.

    Article  Google Scholar 

  • Gossen, B. D., Strelkov, S. E., Manolii, V. P., Rennie, D. C., Cao, T., & Hwang, S. F. (2015). Spread of Plasmodiophora brassicae on canola in Canada, 2003–2014: Old pathogen, new home. Canadian Journal of Plant Pathology, 37, 403–413.

    Article  Google Scholar 

  • Hirai, M. (2006). Genetic analysis of clubroot resistance in brassica crops. Breeding Science, 56, 223–229.

    Article  Google Scholar 

  • Ji, R., Zhao, L., Xing, M., Shen, X., Bi, Q., Peng, S., & Feng, H. (2014). Infection of Plasmodiophora brassicae in Chinese cabbage. Genetics and Molecular Research, 13, 10976–10982.

    Article  CAS  Google Scholar 

  • Kimura, M., & Crow, J. F. (1964). The number of alleles that can be maintained in a finite population. Genetics, 49, 725–738.

  • Kuginuki, Y., Yoshikawa, H., & Hirai, M. (1999). Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassicarapa L. ssp. pekinensis). European Journal of Plant Pathology, 105, 327–332.

    Article  Google Scholar 

  • Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

    Article  CAS  Google Scholar 

  • Laila, R., Robin, A. H. K., Yang, K., Choi, G. J., Jong-In, P., & Nou, I. (2017). Reply to the Letter to the Editor by A. Schwelm and S. Neuhauser: “Detection of Ribosomal DNA Sequence Polymorphisms in the Protist Plasmodiophora brassicae for the Identification of Geographical Isolates”. International Journal of Molecular Sciences, 18, 1455. https://doi.org/10.3390/ijms18071455.

    Article  PubMed Central  Google Scholar 

  • Lewontin, R. C. (1972). The appointment of human diversity. Evolutionary Biology, 6, 381–398.

  • Li, G., & Quiros, C. F. (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 103, 455–461.

    Article  CAS  Google Scholar 

  • Liu, F., Zhang, L. H., & Ji, G. H. (2013). Identification of physiological race of Plasmodiophora brassicae in Yunnan and Tibet. China Vegetable., 20, 77–81.

    Google Scholar 

  • Manzanares-Dauleux, M. J., Barret, P., & Thomas, G. (2000). Development of a pathotype specific SCAR marker in Plasmodiophora brassicae. European Journal of Plant Pathology, 106, 781–787.

    Article  CAS  Google Scholar 

  • Mortazavi, A., Williams, B. A., Mccue, K., Schaeffer, L., & Wold, B. (2008). Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nature Methods, 5, 621–628.

    Article  CAS  Google Scholar 

  • Niwa, R., Kawahara, A., Murakami, H., Tanaka, S., & Ezawa, T. (2011). Complete structure of nuclear rDNA of the obligate plant parasite Plasmodiophora brassicae: Intraspecific polymorphisms in the exon and group I intron of the large subunit rDNA. Protist, 162, 423–434.

    Article  CAS  Google Scholar 

  • Rohlf FJ, (2000). NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.10e. Applied biostatistics Inc, New York.

  • Saitou, N., & Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.

    CAS  PubMed  Google Scholar 

  • Schwelm, A., Berney, C., Dixelius, C., Bass, D., & Neuhauser, S. (2016). The large subunit rDNA sequence of Plasmodiophora brassicae does not contain intra-species polymorphism. Protist., 167, 544–554.

    Article  CAS  Google Scholar 

  • Sharma, K., Gossen, B. D., & McDonald, M. R. (2011). Effect of temperature on cortical infection by Plasmodiophora brassicae and clubroot severity. Phytopathology, 101, 1424–1432.

    Article  Google Scholar 

  • Somé, A., Manzanares, M. J., Laurens, F., Baron, F., Thomas, G., & Rouxel, F. (1996). Variation for virulence on Brassica napus L. amongst Plasmodiophora brassicae collections from France and derived single-spore isolates. Plant Pathology, 45, 432–439.

    Article  Google Scholar 

  • Strelkov, S. E., & Hwang, S. F. (2014). Clubroot in the Canadian canola crop: 10 years into the outbreak. Canadian Journal of Plant Pathology, 36(Suppl 1), 27–36.

    Article  CAS  Google Scholar 

  • Strelkov, S. E., Tewari, J. P., & Smith-Degenhardt, E. (2006). Characterization of Plasmodiophora brassicae populations from Alberta, Canada. Canadian Journal of Plant Pathology, 27, 143–144.

    Google Scholar 

  • Strelkov, S. E., Hwang, S. F., Manolii, V. P., Cao, T., & Feindel, D. (2016). Emergence of new virulence phenotypes of Plasmodiophora brassicae on canola (Brassica napus) in Alberta, Canada. European Journal of Plant Pathology, 145, 517–529.

    Article  Google Scholar 

  • Tanaka, S., & Ito, S. (2013). Pathogenic and genetic diversity in Plasmodiophora brassicae (clubroot) from Japan. Journal of General Plant Pathology, 79, 297–306.

    Article  Google Scholar 

  • Willliams, P. H. (1966). A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology., 56, 624–626.

    Google Scholar 

  • Xue, S., Cao, T., Howard, R. J., Hwang, S. F., & Strelkov, S. E. (2008). Isolation and variation in virulence of single-spore isolates of Plasmodiophora brassicae from Canada. Plant Disease, 92, 456–462.

    Article  CAS  Google Scholar 

  • Zhang, H., Feng, J., Manolii, V. P., Strelkov, S. E., & Hwang, S. F. (2015). Characterization of a gene identified in pathotype 5 of the clubroot pathogen Plamodiaphora brassicae. Phytopathology, 105, 764–770.

    Article  CAS  Google Scholar 

  • Zheng, J., Wang, X., Xiao, Y., Wei, S., Wang, D., Huang, Y., Wang, W., & Yang, H. (2018a). Specific genes identified in pathotype 4 of the clubroot pathogen Plasmodiophora brassicae. Plant Disease. https://doi.org/10.1094/PDIS-05-18-0912-RE.

  • Zheng, J., Wang, X., Li, Q., Yuan, S., Wei, S., Tian, X., Huang, Y., Wang, W., & Yang, H. (2018b). Characterization of five molecular markers for pathotype identification of the clubroot pathogen Plasmodiophora brassicae. Phytopathology. https://doi.org/10.1094/PHYTO-11-17-0362-R.

  • Zuckerkandl, E., & Pauling, L. (1965). Evolutionary divergence and convergence in proteins. Edited in Evolving genes and proteins by V. Bryson and H.J. Vogel, pp. 97–166. Academic press, New York.

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Author notes

  1. H. Yang, J. Zheng and Y. D. Fu contributed equally to this work.

Authors and Affiliations

  1. College of Agronomy, Sichuan Agricultural University Chengdu Campus, Chengdu, 611130, China

    H. Yang, J. Zheng, Y. D. Fu, Y. H. Zhang, C. L. Yi, C. Jin & Y. Huang

  2. Rice Research Institute and Research Center for Major Crop Diseases, Sichuan Agricultural University Chengdu Campus, Chengdu, 611130, China

    Wenming Wang

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  1. H. Yang
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  2. J. Zheng
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  3. Y. D. Fu
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Correspondence to Wenming Wang.

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I certify that this manuscript is original and has not been published and will not be submitted elsewhere for publication while being considered by Plant Pathology. And the study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time. No data have been fabricated or manipulated (including images) to support our conclusions. No data, text, or theories by others are presented as if they were our own. The submission has been received explicitly from all co-authors. And authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Electronic supplementary material

Supplementary Fig. S1

Diagram of genomic region of PBRA_000303, PBRA_006533, and PBRA_009559 (DOCX 74 kb)

Supplementary Fig. S2

The multiple sequence alignment of 34 isolates in SSU between 1180 nt to 1580 nt. (JPG 1210 kb)

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Yang, H., Zheng, J., Fu, Y.D. et al. Specific genes and sequence variation in pathotype 7 of the clubroot pathogen Plasmodiophora brassicae. Eur J Plant Pathol 157, 17–28 (2020). https://doi.org/10.1007/s10658-020-01968-0

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  • DOI: https://doi.org/10.1007/s10658-020-01968-0

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