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Tomato Mi-1.2 gene confers resistance to Meloidogyne luci and M. ethiopica

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Abstract

The root knot nematode (RKN) Meloidogyne luci, recently added to the European Plant Protection Organization Alert List, represents a potential threat to the production of important solanaceous crops. RKN resistance in tomato (Solanum lycopersicum L.) is conferred by a single dominant gene. The objectives were to screen the RKN resistance Mi-1.2 gene in 27 tomato hybrids and cultivars and assess the ability of M. luci and M. ethiopica to reproduce on these accessions. Thirteen Mimi tomato genotypes, two MiMi and twelve lacking the Mi-1.2 gene (mimi) were identified. Tomato accessions Reconquista (MiMi) and Vimeiro F1, Paipai, Sahel, Agora F1, Amaral, Valoasis RZ F1 and SV1917 (Mimi) displayed a resistant reaction to both RKN species with a reproduction factor (Rf) ranging from 0.00 to 0.14. Significant differences in M. luci and M. ethiopica reproduction were detected among and within the Mi-1.2 allelic conditions, which may reflect a dosage effect of the gene as well as an influence of the plant’s genetic background, respectively. Results revealed that resistant tomato accessions carrying the resistant Mi gene have potential to be employed as an alternative to the use of nematicides aiming to control M. luci, and M. ethiopica and the most common RKN species.

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Acknowledgments

The authors thank Dr. Regina Carneiro (EMBRAPA – Recursos Genéticos e Biotecnologia, Brasilia, Brazil) for providing M. ethiopica isolate; and Professor José Paulo Sousa (CFE - Centre for Functional Ecology-Science for People & the Planet, Department of Life Sciences, University of Coimbra) for fruitful suggestion with statistical analysis. This research was supported by CFE, Instituto do Ambiente, Tecnologia e Vida, Chemical Process Engineering and Forest Products Research Centre, Department of Chemical Engineering, UC, and FEDER funds through the Portugal 2020 (PT 2020) Programa Operacional Factores de Competitividade 2020 (COMPETE 2020) and by Fundação para a Ciência e a Tecnologia (FCT, Portugal), under contracts UID/BIA/04004/2019, PEst-UID/EQU/00102/2019, POCI-01-0145-FEDER-031946 (Ref. PTDC/ASP-PLA/31946/2017), POCI-01-0145-FEDER-029392 (Ref. PTDC/ASP-PLA/29392/2017) and Project ReNATURE – Valorization of the Natural Endogenous Resources of the Centro Region (Centro2020, Centro-01-0145-FEDER-000007).

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Correspondence to C. Maleita.

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Santos, D., Martins da Silva, P., Abrantes, I. et al. Tomato Mi-1.2 gene confers resistance to Meloidogyne luci and M. ethiopica. Eur J Plant Pathol 156, 571–580 (2020). https://doi.org/10.1007/s10658-019-01907-8

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Keywords

  • Pest control
  • Resistance genes
  • Root knot nematode
  • SCAR marker
  • Solanum lycopersicum