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Morphological and molecular description of Longidorus behshahrensis n. sp. (Nematoda: Longidoridae) from natural forests of Abbas Abad, north of Iran

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Abstract

Longidorus behshahrensis n. sp. is described and illustrated using morphological and molecular data. It was recovered from the rhizosphere of a wild cherry tree in Mazandaran province, northeastern Iran. The new species is characterized by having 6.8–8.4 mm long females, lip region separated from the rest of the body by a shallow depression, amphidial fovea pocket shaped not bilobed, guiding ring at 25–31 μm from the anterior end, 81–91 and 51–63 μm long odontostyle and odontophore, respectively, 109–130 μm long pharyngeal bulb, didelphic-amphidelphic reproductive system with long tubular bipartite uteri with sperm, vulva at 44–54%, tail bluntly conical, dorsally convex, ventrally concave with widely rounded terminus, hyaline region with discernible radial lines; males common with 50–60 μm long spicules and 9–11 ventromedian single supplements. Only the two latter juvenile developmental stages were recovered for the new species. Codes for identifying the new species are A3-B1-C23-D2-E1-F34-G23-H2-I2. Morphologically, the new species comes close to eight known species of the genus namely L. artemisiae, L. dunensis, L. elongatus, L. euonymus, L. goodeyi, L. iranicus, L. protae and L. tabrizicus. The morphological differences of the new species and the aforementioned species are discussed. In molecular phylogenetic analyses using LSU rDNA D2-D3 sequences, the position of the new species among closely related species was not resolved due to polytomy. In ITS1 phylogeny, the new species formed a clade with L. distinctus.

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Notes

  1. The specific epithet refers to the geographical distribution point of the new species.

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Acknowledgements

We acknowledge the Science and Research Branch of Islamic Azad University (Iran) and Tarbiat Modares University (Iran) for their financial support. The study was partly supported by BAS, ANIDIV3 project.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Shahrbanou Bakhshi Amrei & Farshad Rakhshandehroo

  2. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2, Gagarin Street, 1113, Sofia, Bulgaria

    Vlada Peneva

  3. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Majid Pedram

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  1. Shahrbanou Bakhshi Amrei
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  2. Vlada Peneva
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  3. Farshad Rakhshandehroo
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Correspondence to Majid Pedram.

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The LSU Bayesian tree inferred using maximal number of available data. This tree is used to infer a pruned smaller LSU tree. (JPG 550 kb)

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Bakhshi Amrei, S., Peneva, V., Rakhshandehroo, F. et al. Morphological and molecular description of Longidorus behshahrensis n. sp. (Nematoda: Longidoridae) from natural forests of Abbas Abad, north of Iran. Eur J Plant Pathol 156, 387–398 (2020). https://doi.org/10.1007/s10658-019-01884-y

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