Can the inclusion of uniconazole improve the effectiveness of acibenzolar-S-methyl in managing bacterial speck (Pseudomonas syringae pv. tomato) and bacterial spot (Xanthomonas gardneri) in tomato?
There are reports of acibenzolar-S-methyl (ASM) having host fitness costs and variable levels of control of bacterial speck (Pseudomonas syringae pv. tomato) and bacterial spot (Xanthomonas gardneri) in tomato (Solanum lycopersicum). The plant growth regulator uniconazole (UNI) is associated with alleviating abiotic stress symptoms, and was tested as an additive to ASM to see if it would reduce ASM-associated fitness costs and improve the consistency of disease control. Field applied ASM (fASM) plus greenhouse applied UNI (gUNI) was less consistent than fASM alone, as the combination reduced disease incidence in only two of three years versus fASM alone that reduced disease incidence in three of three years. However, fASM alone never increased total yield compared to the non-treated control, whereas fASM+gUNI increased it in one of three years, which was not associated with changes in disease intensity or relative chlorophyll levels. Greenhouse applied ASM (gASM) plus gUNI reduced disease incidence in one of three years, whereas gASM alone was never effective. This is the first report that gASM can result in long term disease control reducing disease severity up to 13 weeks post-application, indicating long-term effects of gASM are possible. The lack of improved consistency for disease control or improved yield with ASM combined with UNI compared to ASM alone indicates that other additives need to be tested. Also, further research is needed to discover why the ASM + UNI combination did provide improvements under certain field conditions.
KeywordsSystemic acquired resistance Solanum lycopersicum
This work was supported with funding from the Ontario Ministry of Agriculture, Food and Rural Affairs – University of Guelph Partnership Program (P. Goodwin), the Ontario Tomato Research Institute (C. Trueman), Syngenta Canada (C. Trueman), and Valent Canada (C. Trueman).
Compliance with ethical standards
Conflict of interest
C. Trueman has received research grants from Syngenta Canada and Valent Canada, and C. Trueman and S. Loewen have received research grants from the Ontario Tomato Research Institute.
Research involving human participants and/or animals
This research did not involve human participants and/or animals.
- Alexander, S. A., & Waldenmaier, C. M. (2003). Evaluation of fungicides for control of bacterial spot in staked tomatoes, 2002. Fungicide and Nematicide Tests, 58, V056.Google Scholar
- Al-Rumaih, M. M., & Al-Rumaih, M. M. (2007). Physiological response of two species of Datura to uniconazole and salt stress. Journal of Food, Agriculture and Environment, 5(3–4), 450–453.Google Scholar
- Baysal, Ö., Soylu, E. M., & Soylu, S. (2003). Induction of defence-related enzymes and resistance by the plant activator acibenzolar-S-methyl in tomato seedlings against bacterial canker caused by Clavibacter michiganensis ssp. michiganensis. Plant Pathology, 52(6), 747–753. https://doi.org/10.1111/j.1365-3059.2003.00936.x.CrossRefGoogle Scholar
- Bowman, D. T. (2001). Common use of the CV: A statistical aberration in crop performance trials. The Journal of Cotton Science, 5(2), 137–141.Google Scholar
- Cavalcanti, F. R., Resende, M. L. V., Lima, J. P. M. S., Silveira, J. A. G., & Oliveira, J. T. A. (2006). Activities of antioxidant enzymes and photosynthetic responses in tomato pre-treated by plant activators and inoculated by Xanthomonas vesicatoria. Physiological and Molecular Plant Pathology, 68(4–6), 198–208. https://doi.org/10.1016/j.pmpp.2006.11.001.CrossRefGoogle Scholar
- Damicone, J. P., & Trent, M. A. (2003). Evaluation of spray programs for control of bacterial spot and bacterial speck of fresh-market tomato, 2002. Fungicide and Nematicide Tests, 58, V018.Google Scholar
- Duan, L., Guan, C., Li, J., Eneji, A. E., Li, Z., & Zhai, Z. (2008). Compensative effects of chemical regulation with uniconazole on physiological damages caused by water deficiency during the grain filling stage of wheat. Journal of Agronomy and Crop Science, 194(1), 9–14. https://doi.org/10.1111/j.1439-037X.2007.00284.x.CrossRefGoogle Scholar
- Durrant, W. E., & Dong, X. (2004). Systemic acquired resistance. Annual Review of Phytopathology, 42, 185–209. https://doi.org/10.1146/annurev.phyto.42.040803.140421.CrossRefPubMedGoogle Scholar
- Gianquinto, G., Sambo, P., & Borsato, D. (2006). Determination of SPAD threshold values for the optimisation of nitrogen supply in processing tomato. Acta Horticulturae, (700), 159–166.Google Scholar
- Gong, P., Zhang, J., Li, H., Yang, C., Zhang, C., Zhang, X., Khurram, Z., Zhang, Y., Wang, T., Fei, Z., & Ye, Z. (2010). Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction, and biochemical pathways in tomato. Journal of Experimental Botany, 61(13), 3563–3575. https://doi.org/10.1093/jxb/erq167.CrossRefPubMedPubMedCentralGoogle Scholar
- Goodwin, P. H., Trueman, C. L., Loewen, S. A., & Tazhoor, R. (2017). Variation in the response of tomato (Solanum lycopersicum) breeding lines to the effects of benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH) on systemic acquired resistance and seed germination. Journal of Phytopathology, 165(10), 670–680. https://doi.org/10.1111/jph.12606.CrossRefGoogle Scholar
- Griffin, K., Gambley, C., Brown, P., & Li, Y. (2017). Copper-tolerance in Pseudomonas syringae pv. tomato and Xanthomonas spp. and the control of diseases associated with these pathogens in tomato and pepper. A systematic literature review. Crop Protection, 96, 144–150. https://doi.org/10.1016/j.cropro.2017.02.008.CrossRefGoogle Scholar
- Huang, C.-H., Vallad, G. E., Zhang, S., Wen, A., Balogh, B., Figueiredo, J. F. L., et al. (2012). Effect of application frequency and reduced rates of acibenzolar-S-methyl on the field efficacy of induced resistance against bacterial spot on tomato. Plant Disease, 96(2), 221–227. https://doi.org/10.1094/PDIS-03-11-0183.CrossRefPubMedGoogle Scholar
- Jones, J. B. (1991a). Bacterial speck. In J. B. Jones, J. P. Jones, R. E. Stall, & T. A. Zitter (Eds.), Compendium of tomato diseases (Vol. first, pp. 26-27). St. Paul: APS Press.Google Scholar
- Jones, J. B. (1991b). Bacterial spot. In J. B. Jones, J. P. Jones, R. E. Stall, & T. A. Zitter (Eds.), Compendium of tomato diseases (Vol. first, pp. 27). St. Paul: APS Press.Google Scholar
- Koike, S. T., Gladders, P., & Paulus, A. O. (2007). Bacterial spot. In Vegetable diseases: a colour handbook (Vol. first, pp. 332-333). London: Manson Publishing.Google Scholar
- Kunz, W., Schurter, R., & Maetzke, T. (1997). The chemistry of benzothiadiazole plant activators. Pesticide Science, 50(4), 275–282. https://doi.org/10.1002/(SICI)1096-9063(199708)50:4<275::AID-PS593>3.0.CO;2-7.CrossRefGoogle Scholar
- Lange, H. W., Borsick Herman, M. A., & Smart, C. D. (2007). Comparing efficacy of foliar and soil treatments for bacterial speck of tomato, 2006. Plant Disease Management Reports, 1, V009.Google Scholar
- LeBoeuf, J., Cuppels, D., Dick, J., Pitblado, R., Loewen, S., & Celetti, M. (2009). Bacterial diseases of tomato: Bacterial spot, bacterial speck, bacterial canker. http://www.omafra.gov.on.ca/english/crops/facts/05-069.htm. Accessed 23 Feb 2012.
- Lewis Ivey, M. L., Mera, J. R., & Miller, S. A. (2004). Evaluation of fungicides and bactericides for the control of foliar and fruit diseases of processing tomatoes, 2004. Fungicide and Nematicide Tests, 60, V110.Google Scholar
- Mahesaniya, A. (2002). Paclobutrazol and acibenzolar-S-methyl induced tomato seedling growth response and resistance to bacterial speck (Pseudomonas syringae pv. tomato). M.Sc. Thesis, University of Guelph. Guelph; Dept. of Horticultural Science.Google Scholar
- Maymoune, A., Adeline, P., Marie, T., Sophie, G., Sophie, C., Catherine, L., Claire, N., & Sonia, H. (2015). Impact of abiotic stresses on the protection efficacy of defence elicitors and on metabolic regulation in tomato leaves infected by Botrytis cinerea. European Journal of Plant Pathology, 142(2), 223–237. https://doi.org/10.1007/s10658-015-0606-y.CrossRefGoogle Scholar
- Miller, S. A., & Mera, J. R. (2008). Evaluation of fungicide and bactericides for the control of foliar and fruit diseases of processing tomatoes, 2008. Plant Disease Management Reports, V008. https://doi.org/10.1094/PDMR03.
- Miller, S. A., Lewis Ivey, M. L., & Mera, J. (2002). Evaluation of fungicides and plant activators for the control of foliar and fruit disease of processing tomatoes, 2001. Fungicide and Nematicide Tests, 57, V116.Google Scholar
- Monti, L. M. (1980). The breeding of tomatoes for peeling. Acta Horticulturae, (100), 341–353.Google Scholar
- Nir, I. D. O., Moshelion, M., & Weiss, D. (2014). The Arabidopsis GIBBERELLIN METHYL TRANSFERASE 1 suppresses gibberellin activity, reduces whole-plant transpiration and promotes drought tolerance in transgenic tomato. Plant, Cell & Environment, 37(1), 113–123. https://doi.org/10.1111/pce.12135.CrossRefGoogle Scholar
- Pimentel-Gomes, F. (2009). Curso de estatística experimental (15th ed.). Piracicaba: Fundação de Estudos Agrários Luiz de Queiroz.Google Scholar
- Pontes, N. D. C., Nascimento, A. D. R., Golynski, A., Maffia, L. A., Rogério de Oliveira, J., & Quezado-Duval, A. M. (2016). Intervals and number of applications of acibenzolar-s-methyl for the control of bacterial spot on processing tomato. Plant Disease, 100(10), 2126–2133. https://doi.org/10.1094/PDIS-11-15-1286-RE.CrossRefPubMedGoogle Scholar
- Ritchie, D. F. (2000). Bacterial spot of pepper and tomato. The Plant Health Instructor. https://doi.org/10.1094/PHI-I-2000-1027-01.
- Roberts, P. D., Momol, M. T., Ritchie, L., Olson, S. M., Jones, J. B., & Balogh, B. (2008). Evaluation of spray programs containing famoxadone plus cymoxanil, acibenzolar-S-methyl, and Bacillus subtilis compared to copper sprays for management of bacterial spot on tomato. Crop Protection, 27(12), 1519–1526.CrossRefGoogle Scholar
- Srivastava, L. M. (2002). Plant growth and development: Hormones and environment. Amsterdam: Academic Press.Google Scholar
- Stutts, L., Wang, Y., & Stapleton, A. E. (2018). Plant growth regulators ameliorate or exacerbate abiotic, biotic and combined stress interaction effects on Zea mays kernel weight with inbred-specific patterns. Environmental and Experimental Botany, 147, 179–188. https://doi.org/10.1016/j.envexpbot.2017.12.012.CrossRefGoogle Scholar
- Syngenta (2012). Actigard 50WG. http://www.syngentafarm.ca/Labels/Default2.aspx?src=syngentaca. Accessed March 15 2013.
- Taylor, S. L., Payton, M. E., & Raun, W. R. (1999). Relationship between mean yield, coefficient of variation, mean square error, and plot size in wheat field experiments. Communications in Soil Science and Plant Analysis, 30(9), 1439–1447. https://doi.org/10.1080/00103629909370298.CrossRefGoogle Scholar
- Trueman, C. L. (2015). Copper alternatives for management of bacterial spot (Xanthomonas gardneri) and bacterial speck (Pseudomonas syringae pv. tomato) in processing tomatoes. Acta Horticulturae, 1069, 7.Google Scholar
- Valent (n.d.). SUMAGIC plant growth regulator. https://www.valent.ca/valentus/Data/Labels/SumagicPGR_E_Label.pdf. Accessed April 9 2019.
- Van Eerd, L. L., & Loewen, S. A. (2009) Prior winter wheat straw management influences processing tomato yield but not quality. In (823 ed., pp. 121–126): International Society for Horticultural Science (ISHS), Leuven. https://doi.org/10.17660/ActaHortic.2009.823.14.
- Zandstra, J., Dick, J., & Lang, J. (2006). Effect of plant growth regulators on tomato plug plant production, field establishment, maturity, yield & quality. Canadian Journal of Plant Science, 86(5), 1436–1436.Google Scholar
- Zhang, M., Duan, L., Tian, X., He, Z., Li, J., Wang, B., & Li, Z. (2007). Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis, hormones and antioxidant system. Journal of Plant Physiology, 164(6), 709–717. https://doi.org/10.1016/j.jplph.2006.04.008.CrossRefPubMedGoogle Scholar