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Molecular variability of the root-lesion nematode, Pratylenchus loosi (Nematoda: Pratylenchidae), from tea in Iran

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Abstract

During a nematode survey in Iran a population of Pratylenchus loosi from tea was collected and herein described using morphometrics and several molecular markers and compared with the previous description of this species. Thus, the 18S rRNA gene, D2-D3 expansion regions of the 28S rRNA gene, ITS region, mitochondrial COI and partial nuclear hsp90 gene were amplified. According to morphometrics, the Iranian population was very close to previously described populations except for the stylet which was slightly longer. The phylogenetic reconstruction based on the sequences of all markers are also provided confirming the species differentiation and displaying the position of P. loosi and its relationship with closely related species.

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Authors and Affiliations

  1. Plant Protection Department, Faculty of Agricultural Sciences, University of Guilan, Rasht, 4199613769, Iran

    Seyedeh Negin Mirghasemi & Salar Jamali

  2. Istituto per la Protezione Sostenibile delle Piante (IPSP), Consiglio Nazionale delle Ricerche (CNR), S.S. Bari, Via G. Amendola 122/D, 70126, Bari, Italy

    Elena Fanelli, Alberto Troccoli & Francesca De Luca

  3. Faculty of Agricultural Sciences, University of Guilan, Rasht, 4199613769, Iran

    Mohammed Mehdi Sohani

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  1. Seyedeh Negin Mirghasemi
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  2. Elena Fanelli
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  3. Alberto Troccoli
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Correspondence to Francesca De Luca.

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All the authors certify that 1) do not have any actual or potential conflict of interest, 2) the study described is original and has not been published previously, and is not under consideration for publication elsewhere, 3) all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected. We also certify that all authors have reviewed the manuscript and approved the final version of manuscript before submission.

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No specific permits were required for the described studies. Permission for sampling the crop orchards was granted by the landowner. The samples from wild plants were obtained in public areas, forests, and other natural areas studied. The sites are not protected in any way.

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Mirghasemi, S.N., Fanelli, E., Troccoli, A. et al. Molecular variability of the root-lesion nematode, Pratylenchus loosi (Nematoda: Pratylenchidae), from tea in Iran. Eur J Plant Pathol 155, 557–569 (2019). https://doi.org/10.1007/s10658-019-01792-1

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