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Tomato mottle mosaic virus in Brazil and its relationship with Tm-22 gene

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Abstract

Tomato is an important crop, which can be infected by a wide range of pathogens. Among plant viruses, tomato mosaic virus (ToMV) had been described as the most troublesome to tomato crops. Recently, tomato mottle mosaic virus (ToMMV) has gained attention. ToMV and ToMMV are now considered as major threats to tomato. Therefore, the study of some aspects of these viral species would be an advance for the understanding of the disease in the field. In this context, our work aimed: i) to look for possible recombination events in the emergence of ToMMV, ii) to verify the involvement of Tm-22 in the resistance against ToMMV, and iii) to evaluate the behavior of ToMV and ToMMV in tomato cultivars and hybrid lines. Our results revealed no recombination events involving ToMMV. Also, our results suggested that Tm-22 confers resistance to ToMMV. Brazilian commercial cultivars and hybrid lines ‘Alambra’, ‘Débora Max’, ‘Santa Clara’, ‘Santa Cruz’, ‘Bubble Candy’, ‘Kada’ and ‘Salada’ were evaluated with ToMMV and ToMV. Only ‘Alambra’ showed partial resistance to ToMMV and ToMV and ‘Debora Max’ to ToMV. Finally, it should be noted that these commercial tomato cultivars and hybrids tested were more susceptible to the Brazilian ToMMV isolate when compared to ToMV since the symptoms were much more drastic. The results presented here have important implications for breeding virus-resistant tomatoes.

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Acknowledgements

We thank Dr. Andrew Thompson for providing the seeds of the Ailsa Craig with the Tm-22 allele. We also thank Dr. Pedro Luís Ramos-González for the help with the recombination analyses.

Funding

This study was funded by Coordination for the Improvement of Higher Education Personnel (CAPES Finance Code 001, Brazil) and by the National Council for Scientific and Technological Development (CNPq) (both from departmental quota).

Author information

Authors and Affiliations

  1. Laboratório de Fitoquímica, Instituto de Biociências, Departamento de Botânica, Universidade de São Paulo, R. do Matão, 277., São Paulo, SP, 05508-090, Brazil

    Alice Nagai & Déborah Y. A. C. dos Santos

  2. Laboratório de Fitovirologia, Centro de Pesquisa de Sanidade Vegetal, Instituto Biológico, Av. Cons. Rodrigues Alves, 1252, São Paulo, SP, 04014-002, Brazil

    Lígia M. L. Duarte & Alexandre L. R. Chaves

  3. Laboratório de Controle Hormonal do Desenvolvimento Vegetal, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Av. Padua Dias, 11 CP. 09, Piracicaba, SP, 13418-900, Brazil

    Lázaro E. P. Peres

Authors
  1. Alice Nagai
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  2. Lígia M. L. Duarte
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  3. Alexandre L. R. Chaves
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  4. Lázaro E. P. Peres
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  5. Déborah Y. A. C. dos Santos
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Contributions

Dr. Duarte and Dr. Chaves have performed all the experiments involving the inoculation of both viruses and they have collected the samples to the PTA analyses.

Dr. Nagai has performed the PTA and recombination analyses.

Dr. Peres has suggested the work with NIL of S. lycopersicum to analyze the involvement of Tm-22 allele in the resistance against ToMMV, providing the seeds.

Dr. Chaves and Dr. Peres have contributed to the agronomic approach of the work.

Dr. Santos has supervised all the work, reading and correcting the paper, making it easier to understand.

Corresponding author

Correspondence to Alice Nagai.

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Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The research did not involve human participants and/or animals.

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Nagai, A., Duarte, L.M.L., Chaves, A.L.R. et al. Tomato mottle mosaic virus in Brazil and its relationship with Tm-22 gene. Eur J Plant Pathol 155, 353–359 (2019). https://doi.org/10.1007/s10658-019-01762-7

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  • DOI: https://doi.org/10.1007/s10658-019-01762-7

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