Abstract
Kiwi fruit (Actinidia spp.) is one of the most important fruit crops in the world. Shaanxi is the largest kiwi fruit growing area in China. In this study, field investigation and virus detection showed that Citrus leaf blotch virus (CLBV) commonly occurred in Shaanxi Province and the average incidence rate was 28.53%. In total, some 60 complete nucleotide sequences of movement protein (MP) and coat protein (CP) genes of CLBV were obtained from different regions of Shaanxi. Phylogenetic analysis showed that CLBV had obvious host specialization. The study of CLBV isolates in kiwi fruit preliminarily revealed that CLBV was not significantly associated with the host’s geographic location. The results provide important information for further clarifying the distribution and genetic diversity of CLBV in regions of kiwi fruit production in Shaanxi and lay the foundation for in-depth study of the molecular characteristics and epidemiology of the virus.
References
Adams, M. J., Candresse, T., Hammond, J., Kreuze, J. F., Martelli, G. P., Namba, S., Pearson, M. N., Ryu, K. H., Saldarelli, P., & Yoshikawa, N. (2012). Family Betaflexiviridae. In A. M. Q. King, M. J. Adams, E. B. Carstens, & E. J. Lefkowitz (Eds.), Virus taxonomy: Ninth report of the international committee on taxonomy of viruses (pp. 920–941). London: Elsevier Academic Press.
Aguero, J., Vives, M. C., Velazquez, K., Ruiz-Ruiz, S., Juarez, J., Navarro, L., Moreno, P., & Guerri, J. (2013). Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. Molecular Plant Pathology, 14, 610–616.
Blouin, A. G., Pearson, M. N., Chavan, R. R., Woo, E. N. Y., Lebas, B. S. M., Veerakone, S., Ratti, C., Biccheri, R., MacDiarmid, R. M., & Cohen, D. (2013). Viruses of kiwifruit (Actinidia species). Journal of Plant Pathology, 95, 221–235.
Blouin, A. G., Biccheri, R., Khalifa, M. E., Pearson, M. N., Pollini, C. P., Hamiaux, C., & Ratti, C. (2018). Characterization of Actinidia virus 1, a new member of the family Closteroviridae encoding a thaumatin-like protein. Archives of Virology, 163, 229–234.
Cao, M. J., Yu, Y. Q., Tian, X., Yang, F. Y., Li, R. H., & Zhou, C. Y. (2017). First report of Citrus leaf blotch virus in lemon in China. Plant Disease, 101, 1561–1561.
Chavan, R. R., Blouin, A. G., Cohen, D., & Pearson, M. N. (2013). Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis. Archives of Virology, 158, 1679–1686.
Ferrante, P., & Scortichini, M. (2014). Frost promotes the pathogenicity of Pseudomonas syringae pv. Actinidiae in Actinidia chinensis and A. deliciosa plants. Plant Pathology, 63, 12–19.
Gress, J. C., Smith, S., & Tzanetakis, I. E. (2017). First report of Citrus leaf blotch virus in Peony in the USA. Plant Disease, 101, 637–637.
Guardo, M., Sorrentino, G., Marietta, T., & Caruso, A. (2007). First report of Citrus leaf blotch virus on kumquat in Italy. Plant Disease, 91, 1054–1054.
Guardo, M., Potere, O., Castellano, M. A., Savino, V., & Caruso, A. (2009). A new herbaceous host of Citrus leaf blotch virus. Journal of Plant Pathology, 91, 485–488.
Heath, L., van der Walt, E., Varsani, A., & Martin, D. P. (2006). Recombination patterns in aphthoviruses mirror those found in other picornaviruses. Journal of Virology, 80, 11827–11832.
Li, Z., Kondo, H., Andika, I. B., Liu, P., Sun, L., & Wu, Y. (2016a). Identification of genome recombination among apple stem pitting virus isolates. Journal of Plant Pathology, 98, 595–601.
Li, Y., Zhong, Y., Huang, K., & Cheng, Z. M. (2016b). Genomewide analysis of NBS-encoding genes in kiwi fruit (Actinidia chinensis). Journal of Genetics, 95, 997–1001.
Li, L., Pan, H., Chen, M. Y., Zhang, S. J., & Zhong, C. H. (2017). First report of anthracnose caused by Colletotrichum gloeosporioides on kiwifruit (Actinidia chinensis) in China. Plant Disease, 101, 2151–2151.
Li, P., Li, M., Zhang, S., Wang, J., Yang, F. Y., Cao, M. J., & Li, Z. A. (2018). Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China. Journal of Integrative Agriculture, 17, 712–715.
Liu, P., Li, Z., Song, S., & Wu, Y. (2014). Molecular variability of apple chlorotic leaf spot virus in Shaanxi, China. Phytoparasitica, 42, 445–454.
Marais, A., Faure, C., Mustafayev, E., & Candresse, T. (2015). Characterization of new isolates of apricot vein clearing-associated virus and of a new Prunus-infecting virus: Evidence for recombination as a driving force in Betaflexiviridae evolution. PLoS One, 10, e0129469.
Martelli, G. P., Adams, M. J., Kreuze, J. F., & Dolja, V. V. (2007). Family Flexiviridae: A case study in virion and genome plasticity. Annual Review of Phytopathology, 45, 73–100.
Veerakone, S., Liefting, L. W., Tang, J., & Ward, L. I. (2018). The complete nucleotide sequence and genome organisation of a novel member of the family Betaflexiviridae from Actinidia chinensis. Archives of Virology, 163, 1367–1370.
Vives, M. C., Galipienso, L., Navarro, L., Moreno, P., & Guerri, J. (2001). The nucleotide sequence and genomic organization of Citrus leaf blotch virus: Candidate type species for a new virus genus. Virology, 287, 225–233.
Vives, M. C., Galipienso, L., Navarro, L., Moreno, P., & Guerri, J. (2002). Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus. Virology, 295, 328–336.
Wang, K. X., Xie, Y. L., Yuan, G. Q., Li, Q. Q., & Lin, W. (2015). First report of root and collar rot caused by Phytopythium helicoides on kiwifruit (Actinidia chinensis). Plant Disease, 99, 725–726.
Wang, Y. X., Hong, N., Wang, G. P., Yang, Z. K., Wang, L. P., & Li, L. (2016a). First report of the Tospovirus tomato necrotic spot associated virus infecting kiwifruit (Actinidia sp.) in China. Plant Disease, 100, 2539.
Wang, J., Zhu, D., Tan, Y., Zong, X., Wei, H., & Liu, Q. (2016b). First report of Citrus leaf blotch virus in sweet cherry. Plant Disease, 100, 1027–1027.
Wang, D., Liu, X. X., Li, T. T., Li, Q., Liu, Y., Gong, G. S., & Yang, H. (2018a). First report of cucumber mosaic virus infection in kiwifruit (Actinidia chinensis) in China. Plant Disease, 102, 1180–1180.
Wang, Y. X., Zhuang, H. Q., Yang, Z. K., Wen, L. H., Wang, G. P., & Hong, N. (2018b). Molecular characterization of an apple stem grooving virus isolate from kiwifruit (Actinidia chinensis) in China. Canadian Journal of Plant Pathology, 40, 76–83.
Wu, B. L., Blanchard-Letort, A., Liu, Y., Zhou, G. H., Wang, X. F., & Elena, S. F. (2011). Dynamics of molecular evolution and Phylogeography of barley yellow dwarf virus-PAV. PLoS One, 6.
Zheng, Y. Z., Navarro, B., Wang, G. P., Wang, Y. X., Yang, Z. K., Xu, W. X., Zhu, C. X., Wang, L. P., Di Serio, F., & Hong, N. (2017). Actinidia chlorotic ringspot-associated virus: A novel emaravirus infecting kiwifruit plants. Molecular Plant Pathology, 18, 569–581.
Zhu, C. X., Wang, G. P., Zheng, Y. Z., Yang, Z. K., Wang, L. P., Xu, W. N., & Hong, N. (2016). RT-PCR detection and sequence analysis of coat protein gene of Citrus leaf blotch virus infecting kiwifruit trees (in Chinese). Acta Phytopathologica Sinica, 46, 11–16.
Funding
This study was supported by key research and development plan in shaanxi province(2018ZDXM-NY-058) and the 111 Project from the Education Ministry of China (B07049).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed.
Rights and permissions
About this article
Cite this article
Liu, H., Song, S., Wu, W. et al. Distribution and molecular characterization of Citrus leaf blotch virus from Actinidia in Shaanxi province, China. Eur J Plant Pathol 154, 855–862 (2019). https://doi.org/10.1007/s10658-019-01677-3
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-019-01677-3