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In vitro reproduction of Pratylenchus neglectus on carrot discs and investigation of its interaction with Meloidogyne hispanica on potato

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Abstract

Root-lesion nematodes, Pratylenchus spp., and root-knot nematodes, Meloidogyne spp., can occur concomitantly in potato fields and the interactions between the two groups of plant parasitic nematodes (PPN) may represent a threat to the potato crop. The in vitro reproductive fitness of 3 Portuguese isolates of P. neglectus isolated from potato fields was assessed using monoxenic carrot disc cultures inoculated with 10 females and maintained at 25 °C. The three isolates were able to reproduce at 56 days after inoculation (DAI) and the final population densities were significantly increased at 84 DAI. The P. neglectus isolate with the best reproductive ability was further selected and the concomitant effects of P. neglectus and M. hispanica were investigated in a pot trial using potato (Solanum tuberosum L.) cv. Agria as host. Single potato sprouts were inoculated with 250, 500, 750 and 1000 of either P. neglectus mixed stages/M. hispanica second-stage juveniles, or a combination of both nematodes. At 60 DAI, nematodes were extracted from roots and soil, the final population densities determined, for both nematode species, and the reproductive factor calculated. The experiment was run twice. The results suggest an additive effect of M. hispanica on P. neglectus reproduction, although future tests are still needed to confirm the possibility of an increase of P. neglectus population densities for the next crop, when both nematodes are inoculated concomitantly. The information obtained reinforces the need for a careful monitoring of these two PPN species in potato crops.

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References

  • Acosta, N., & Malek, B. (1979). Influence of temperature on population development of eight species of Pratylenchus on soybean. Journal of Nematology, 11, 229–232.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Al-Rehiayani, S., Hafez, S. L., Thornton, M., & Sundararaj, P. (1999). Effects of Pratylenchus neglectus, Bacillus megaterium, and oil radish or rapeseed green manure on reproductive potential of Meloidogyne chitwoodi on potato. Nematropica, 29, 37–49.

    Google Scholar 

  • Andrés, M. F., Pinochet, J., Hernandez-Dorrego, A., & Delibes, A. (2000). Detection and analysis of inter- and intraspecific diversity of Pratylenchus spp. using isozyme markers. Plant Pathology, 49, 640–649.

    Article  Google Scholar 

  • Castillo, P., & Vovlas, N. (2007). Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, biology, pathogenicity and management (nematology monographs and perspectives). Leiden: Koninklijke Brill.

    Book  Google Scholar 

  • Castillo, P., Trapero-Casas, J. L., & Jiménez-Díaz, R. M. (1995). Effect of time, temperature, and inoculum density on reproduction of Pratylenchus thornei in carrot disk cultures. Journal of Nematology, 27, 120–124.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Castillo, P., Vovlas, N., & Jiménez-Díaz, R. M. (1998). Pathogenicity and histopathology of Pratylenchus thornei populations on selected chickpea genotypes. Plant Pathology, 47, 370–376.

    Article  Google Scholar 

  • Conceição, I. L. P. M. da, Cunha, M. J. M. da, Feio, G., et al. (2009). Root-knot nematodes, Meloidogyne spp., on potato in Portugal. Nematology, 11, 311–313.

  • Davis, J. R., Hafez, S. L., & Sorensen, L. H. (1992). Lesion nematode suppression with the butte potato and relationships to Verticillium wilt. American Potato Journal, 69, 371–383.

    Article  Google Scholar 

  • Esteves, I., Maleita, C., & Abrantes, I. (2015). Root-lesion and root-knot nematodes parasitizing potato. European Journal of Plant Pathology, 141, 397–406.

    Article  CAS  Google Scholar 

  • Forge, T. A., Larney, F. J., Kachuk, L. M., Pearson, D. C., Koch, C., & Blackshaw, R. E. (2015). Crop rotation effects on Pratylenchus neglectus populations in the root zone of irrigated potatoes in southern Alberta. Canadian Journal of Plant Pathology, 37, 363–368.

    Article  Google Scholar 

  • Fourie, H., Venter, G. A., & Mc Donald, A. H. (2003). Optimum temperature and inoculation levels for in vitro production of Pratylenchus brachyurus on carrot discs. Journal of Plant and Soil, 20, 44–45.

    Article  Google Scholar 

  • Gonzaga, V., & Santos, J. M. (2010). Estudo comparativo in vitro de seis espécies de Pratylenchus em cilindros de cenoura. Nematologia Brasileira, 34, 228–230.

    Google Scholar 

  • Hafez, S. L., Al-Rehiayani, S., Thornton, M., & Sundararaj, P. (1999). Differentiation of two geographically isolated populations of Pratylenchus neglectus based on their parasitism of potato and interaction with Verticillium dahliae. Nematropica, 29, 25–35.

    Google Scholar 

  • Hussey, R. S., & Barker, K. R. (1973). A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57, 1025–1028.

    Google Scholar 

  • Kagoda, F., Coyne, D., Mbiru, E., Derera, J., & Tongoona, P. (2010). Monoxenic culture of Pratylenchus zeae on carrot discs. Nematologia Mediterranea, 38, 107–108.

    Google Scholar 

  • Karakas, M. (2015). Reproductive potential of the root-lesion nematode Pratylenchus penetrans (Cobb, 1917) Sher and Allen, 1953 (Nematoda:Pratylenchidae) in Monoxenic cultures. European International Journal of Science and Technology, 4, 105–110.

    Google Scholar 

  • Kinloch, R. A., & Allen, M. W. (1972). Interaction of Meloidogyne hapla and M. javanica infecting tomato. Journal of Nematology, 4, 7–16.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Mahran, A., Tenuta, M., Shinners-Carenelly, T., Mundo-Ocampo, M., & Daayf, F. (2010). Prevalence and species identification of Pratylenchus spp. in Manitoba potato fields and host suitability of ‘Russet Burbank’. Canadian Journal of Plant Pathology, 32, 272–282.

    Article  CAS  Google Scholar 

  • Maleita, C. M., Curtis, R. H. C., & Abrantes, I. M. de O. (2012a). Thermal requirements for the embryonic development and life cycle of Meloidogyne hispanica. Plant Pathology, 61, 1002–1010.

    Article  Google Scholar 

  • Maleita, C., Curtis, R., Powers, S., & Abrantes, I. (2012b). Host status of Meloidogyne hispanica. European Journal of Plant Pathology, 133, 449–460.

    Article  Google Scholar 

  • Maleita, C., Esteves, I., Cardoso, J. M. S., Cunha, M. J., Carneiro, R. M. D. G., & Abrantes, I. (2018). Meloidogyne luci, a new root-knot nematode parasitizing potato in Portugal. Plant Pathology, 67, 366–376. https://doi.org/10.1111/ppa.12755.

    Article  CAS  Google Scholar 

  • Mokrini, F., Waeyenberge, L., Viaene, N., Andaloussi, F. A., & Moens, M. (2016). Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco. Nematology, 18, 781–801.

    Article  Google Scholar 

  • Mudiope, J., Coyne, D., Adipala, E., & Sikora, R. A. (2004). Monoxenic culture of the root-lesion nematode Pratylenchus sudanensis on carrot disks. Nematology, 6, 617–619.

    Article  Google Scholar 

  • Nyczepir, A. P. (2009). Dynamics of concomitant populations of Pratylenchus vulnus and Meloidogyne incognita on peach. Nematropica, 39, 273–279.

    Google Scholar 

  • O'Bannon, J. H., & Taylor, A. L. (1968). Migratory endoparasitic nematodes reared on carrot discs. Phytopathology, 58, 385.

    Google Scholar 

  • Olthof, T. H. A. (1990). Reproduction and parasitism of Pratylenchus neglectus on potato. Journal of Nematology, 22, 303–308.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pang, W., Hafez, S. L., & Sundararaj, P. (2009). Concomitant interaction of Pratylenchus penetrans and Meloidogyne hapla on onion. Nematropica, 39, 297–303.

    Google Scholar 

  • Pinochet, J., Cenis, J. L., Fernandez, C., Doucet, M., & Marull, J. (1994). Reproductive fitness and random amplified polymorphic DNA variation among isolates of Pratylenchus vulnus. Journal of Nematology, 26, 271–277.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Taylor, A. L., & Sasser, J. N. (1978). Biology, identification and control of root-knot nematodes (Meloidogyne spp.). A cooperative publication of the Department of Plant Pathology, North Carolina State University and the United States Agency for International Development, Raleigh: North Carolina State University Graphics.

  • Umesh, K. C., & Ferris, H. (1994). Influence of temperature and host plant on the interaction between Pratylenchus neglectus and Meloidogyne chitwoodi. Journal of Nematology, 26, 65–71.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Van Der Sommen, A. T. C., Van Bruggen, A. S. & Den Nijs, L. J. M. F. (2009). Survey of poorly growing patches in potato fields by helicopter. In International Congress of Tropical Nematology (40th ONTA and 28th SBN meetings), Maceió, Brazil.

  • Verdejo-Lucas, S., & Pinochet, J. (1992). Population densities of five migratory endoparasitic nematodes in carrot disk cultures. Journal of Nematology, 24, 96–98.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Viaene, N. (2014). Nematodes: an increasing threat for the potato crop in Europe? Potato Research, 57, 335–338.

    Article  Google Scholar 

  • Vovlas, N., Troccoli, A., Minuto, A., Bruzzone, C., & Castillo, P. (2010). Parasitism of the root-knot nematode Meloidogyne hapla on peony in northern Italy. Nematologia Mediterranea, 38, 219–221.

    Google Scholar 

  • Whitehead, A. G., & Hemming, J. R. (1965). A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55, 25–38.

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by CFE, Instituto do Ambiente, Tecnologia e Vida, CIEPQPF and FEDER funds through the Programa Operacional Factores de Competitividade (COMPETE, Portugal) and by FCT (Fundação para a Ciência e a Tecnologia, Portugal), under contracts UID/BIA/04004/2013, PEst-C/EQB/UI0102/2013, FCOMP-01-0124-FEDER-027960 (Ref. PTDC/AGRPRO/2510/2012), POCI-01-0145-FEDER-029392 and Project ReNATURE – Valorization of the Natural Endogenous Resources of the Centro Region (Centro 2020, Centro-01-0145-FEDER-000007). Carla Maleita (SFRH/BPD/85736/2012) and Ivânia Esteves (SFRH/BPD/68856/2010) acknowledge FCT for granted fellowships, financed by Ministério da Educação e Ciência national funding and by the European Social Funds through Programa Operacional Capital Humano.

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Author notes

  1. Ivânia Esteves and Carla Maleita contributed equally to this work.

Authors and Affiliations

  1. CFE – Centre for Functional Ecology - Science for People & the Planet, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, P-3000 456, Coimbra, Portugal

    Ivânia Esteves, Carla Maleita & Isabel Abrantes

  2. CIEPQPF – Chemical Process Engineering and Forest Products Research Centre, Chemical Engineering Department, University of Coimbra, Rua Sílvio Lima, Pólo II, Pinhal de Marrocos, P-3030 790, Coimbra, Portugal

    Carla Maleita

  3. CERNAS – Centro de Estudos em Recursos Naturais, Ambiente e Sociedade, Department of Agronomic Sciences, High School of Agriculture, Instituto Politécnico de Coimbra, Bencanta, P-3045 601, Coimbra, Portugal

    Tânia Pato & Maria José Cunha

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  1. Ivânia Esteves
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  2. Carla Maleita
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  4. Isabel Abrantes
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  5. Maria José Cunha
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Correspondence to Ivânia Esteves or Carla Maleita.

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Esteves, I., Maleita, C., Pato, T. et al. In vitro reproduction of Pratylenchus neglectus on carrot discs and investigation of its interaction with Meloidogyne hispanica on potato. Eur J Plant Pathol 153, 957–963 (2019). https://doi.org/10.1007/s10658-018-1582-9

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