Characterization of Fusarium spp. isolates recovered from bananas (Musa spp.) affected by Fusarium wilt in Puerto Rico
- 246 Downloads
Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (FOC), is one of the most destructive diseases of bananas (Musa spp.) worldwide. Although the first report of this disease in Puerto Rico was in 1919, little has been published since that time. We report the first systematic description of FOC on the island. A total of 28 single-spore isolates of Fusarium spp. were recovered from symptomatic bananas from several municipalities. Species identity of the isolates was initially based on morphology and later confirmed with partial sequences of the translation elongation factor 1α (TEF-1α) gene. Isolates were examined for vegetative compatibility, race identity using PCR primers reported for different races, and virulence on tissue-culture-derived plantlets of differential cultivars for races 1, 2 and 4. Most isolates of F. oxysporum belonged to VCG 0124 of FOC, and amplified with primers designed for race 1. Several isolates amplified with primers developed for race 4, but no isolate amplified with primers specific for tropical race 4 (TR4). Most isolates produced symptoms on race 1 or race 2 susceptible cultivars. Although some isolates caused minor symptoms on ‘Gran Nain’, disease severities on this race 4 differential were considerably lower than those in susceptible race 1 or race 2 interactions. Based on these results, race 1 and race 2, but not TR4, are present in Puerto Rico. We discuss the disease and FOC’s population structure in Puerto Rico, as well as the reliability of different PCR primers that have been developed to identify races of this pathogen.
KeywordsFusarium Musa Banana Vegetative compatibility group
The authors thank staff at the USDA-ARS Tropical Agricultural Research Station (TARS) for their technical assistance. Special thanks go to Ms. Yaleidis Mendez and Ms. Sirena Montalvo, of the TARS, and Mr. Fernando Garcia-Bastidas and Dr. Gert Kema, of Wageningen University, for their help with optimization and conducting of pathogenicity tests and to Mr. Bernardo Chavez-Cordoba for his help with the statistical analyses. This work was carried out as part of collaboration between USDA-ARS TARS and the UPR Agricultural Experiment Station project no. Z-277.
Compliance with ethical standards
Conflict of interest
Authors have all participated in research and/or manuscript preparation, have all reviewed and approved submission and declare no conflicts of interest.
Human and animals rights
Research did not involve human participants and/or animals.
- Aguayo, J., Mostert, D., Fourrier-Jeandel, C., Cerf-Wendling, I., Hostachy, B., Viljoen, A., & Ioos, R. (2017). Development of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp. cubense race 4. PLoS One, 12, e0171767. https://doi.org/10.1371/journal.pone.0171767.CrossRefPubMedPubMedCentralGoogle Scholar
- Armitage, A. D., Taylor, A., Sobczyk, M. K., Baxter, L., Greenfield, B. P., Bates, H. J., et al. (2017). Pangenomic analysis reveals pathogen-specific regions and novel effector candidates in Fusarium oxysporum f. sp. cepae. bioRxiv. https://doi.org/10.1101/182238.
- Bancroft, J. (1876). Report of the board appointed to inquire into to the cause of disease affecting livestock and plants, Queensland, 1876. In Votes and Proceedings 3, 1011–1038.Google Scholar
- Brandes, E. W. (1919). Banana wilt. Phytopathology, 9, 339–389.Google Scholar
- Cortes, M., & Gayol, L. (2006). Componentes de la empresa de plátanos en Puerto Rico. Journal of Agriculture of the University of Puerto Rico, 90, 3–4.Google Scholar
- Czislowksi, E., Fraser-Smith, S., Zander, M., O’Neill, W. T., Meldrum, R. A., Tran-Nguyen, L. T. T., et al. (2017). Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer. Molecular Plant Pathology, 19, 1155–1171. https://doi.org/10.1111/mpp.12594.CrossRefGoogle Scholar
- Deloitte report. (2015). Assessment of the end to end sampling and diagnostic process related to Panama disease Tropical Race 4. https://www.daf.qld.gov.au/__data/assets/pdf_file/0019/335323/panama-report.pdf. Accessed 1 Oct 2017.
- FAOSTAT. (2013). http://faostat.fao.org
- Li, C. Y., Mostert, G., Zuo, C. W., Beukes, I., Yang, Q. S., et al. (2016). Diversity and distribution of the banana wilt pathogen Fusarium oxysporum f. sp. cubense in China. Fungal Genomics & Biology. https://doi.org/10.4172/2165-8056.1000111.
- Liu, J., Chen., X., Wang, B., He, Z., Peng, A., & Cai, M. (2006). Race identification and SCAR markers development for the pathogen of banana Panama disease Fusarium oxysporum f. sp. cubense. Acta Phytopathologica Sinica, 36, 28–34.Google Scholar
- Lule, M., Dubois, T., Coyne, D., Kisitu, D., Kamusiime, H., & Bbemba, J. (2013). Trainer’s manual. A training course for banana farmers interested in growing tissue culture bananas. Ibadan: International Institute of tropical Agriculture.Google Scholar
- Magdama, F. A., & Jimenez-Gasco, M. (2015). Populations of Fusarium oxysporum f. sp. cubense causing Panama disease of banana in Ecuador: Learning from the past for future perspectives. Phytopathology, 105, S4.87.Google Scholar
- Mostert, D., Molina, A. B., Daniells, J., Fourie, G., Hermanto, C., Chao, C.-P., Fabregar, E., Sinohin, V. G., Masdek, N., Thangavelu, R., Li, C., Yi, G., Mostert, L., & Viljoen, A. (2017). The distribution and host range of the banana Fusarium wilt fungus, Fusarium oxysporum f. sp. cubense, in Asia. PLoS One, 12, e0181630. https://doi.org/10.1371/journal.pone.0181630.CrossRefPubMedPubMedCentralGoogle Scholar
- O’Donnell, K., Kistler, H. C., Cigelnik, E., & Ploetz, R. C. (1998). Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences, 95, 2044–2049.CrossRefGoogle Scholar
- Ploetz, R. C. (2000). Panama disease: a classic and destructive disease of banana. Plant Health Progress. https://doi.org/10.1094/PHP-2000-1204-01-HM.
- Ploetz, R. C. (2005). Panama disease: an old nemesis rears its ugly head part 2: the Cavendish era and beyond. APSnet Features. http://www.apsnet.org/publications/apsnetfeatures/Pages/PanamaDiseasePart2.aspx Accessed 1 Oct 2017.
- Ploetz, R. C. (2018). Fusarium wilt. In D. R. Jones (Ed.), Diseases of banana, Abacá and Enset (2nd ed.). Wallingford: CABI Publishing in press.Google Scholar
- Ploetz, R. C., & Evans, E. A. (2015). The future of banana production. Horticultural Reviews, 43, 311–351.Google Scholar
- Ploetz, R. C., & Pegg, K. G. (2000). Fusarium wilt. In D. R. Jones (Ed.), Diseases of banana, Abacá and Enset (1st ed., pp. 143–159). Wallingford: CABI Publishing.Google Scholar
- Simmonds, N. W. (1966). Bananas (2nd ed.). London: Longmans.Google Scholar
- Stover, R. H. (1962). Fusarial wilt (Panama disease) of bananas and other Musa species. Surrey: The Commonwealth Mycological Institute Kew 117pp.Google Scholar
- Stover, R. H. (1990). Fusarium wilt of banana: some history and current status of the disease. In R. C. Ploetz (Ed.), Fusarium wilt of banana (pp. 1–7). St. Paul: APS Press.Google Scholar
- USDA NASS (2012). Census of Agriculture: Puerto Rico Island and Municipio Data. Volume 1. Geographic area series, part 52. AC-12-A-52.Google Scholar
- Waite, B. H. (1963). Wilt of Heliconia spp. caused by Fusarium oxysporum f. sp. cubense race 3. Tropical Agriculture, 40, 299–305.Google Scholar
- Yang, L. L., Sun, L. X., Ruan, X. L., Qiu, D. Y., Chen, D. H., Cai, X. Q., & Li, H. P. (2015). Development of a single-tube duplex real-time fluorescence method for the rapid quantitative detection of Fusarium oxysporum f. sp. cubense race 1 (FOC1) and race 4 (FOC4) using TaqMan probes. Crop Protection, 68, 27–35.CrossRefGoogle Scholar