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European Journal of Plant Pathology

, Volume 152, Issue 2, pp 343–354 | Cite as

Molecular diversity in Fusarium oxysporum isolates from common bean fields in Brazil

  • Andre Freire Cruz
  • Lucas Fagundes Silva
  • Tiago Vieira Sousa
  • Alessandro Nicoli
  • Trazilbo Jose de Paula Junior
  • Eveline Teixeira Caixeta
  • Laercio Zambolim
Article
  • 150 Downloads

Abstract

The common bean (Phaseolus vulgaris L.) is widely cultivated in Brazil and is known as a very important crop for families in this country. Fusarium wilt severely harms common beans and has become a big issue for this crop. In order to assist the breeding programs that target resistance to this disease, the evaluation of genetic diversity of the pathogen and its molecular characterization are crucial. Thus, the present goal was to identify Fusarium isolates obtained from several places in Brazil using molecular tools; select molecular markers for these isolates; and analyze their diversity. All of isolates were molecularly identified as Fusarium oxysporum f. sp. phaseoli (Fop). By using seven selected SSR markers, the results of diversity obtained by the dendrogram and the Bayesian analysis formed four groups where a large diversity of this fungus was found within each state. However, the groups were more homogenous according to the collection source and the pathogenicity test. More specifically, group 2 was composed of the most virulent strains and originated from Minas Gerais State – UFV, and group 3 was mostly composed by isolates from Goias state. Group I was also more diverse in terms of location and virulence. The overall results indicated a positive correlation between Fusarium diversity and its virulence to common bean. Furthermore, the use of these markers was effective in molecular identification and in detecting polymorphism within F. oxysporum f. sp. phaseoli.

Keywords

Diversity Fusarium Genetic Molecular markers Phaseolus vulgaris 

Notes

Acknowledgements

This research was supported by Research Support Foundation of Minas Gerais (FAPEMIG) - Brazil and the National Council for Scientific and Technological Development (CNPq) - Brazil. Thanks also to laboratory of Coffee Biotechnology (Universidade Federal de Vicosa - UFV) for their assistance during the experiment. The authors also thank the Instituto Agronomico de Campinas (IAC), Universidade Federal de Lavras (UFLA) - Laboratory of Seed Pathology, and Embrapa Arroz e Feijao for provide the isolates. Finally the authors would like to express their gratitude to Prof, Dr. Cosme Damiao da Cruz and the laboratory of Bioinformatics for help with the statistical analysis.

Supplementary material

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ESM 1 (PPTX 287 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Andre Freire Cruz
    • 1
    • 2
  • Lucas Fagundes Silva
    • 3
  • Tiago Vieira Sousa
    • 4
  • Alessandro Nicoli
    • 3
    • 5
  • Trazilbo Jose de Paula Junior
    • 2
  • Eveline Teixeira Caixeta
    • 6
  • Laercio Zambolim
    • 3
  1. 1.Graduate School of Life and Environmental SciencesKyoto Prefectural UniversityKyotoJapan
  2. 2.Empresa de Pesquisa Agropecuaria de Minas GeraisBelo HorizonteBrazil
  3. 3.Departmento de FitopatologiaUniversidade Federal de ViçosaViçosaBrazil
  4. 4.Departmento de FitotecniaUniversidade Federal de VicosaViçosaBrazil
  5. 5.Instituto de Ciencias AgrariasUniversidade Federal dos Vales do Jequitinhonha e MucuriUnaiBrazil
  6. 6.Empresa Brasileira de Pesquisa AgropecuáriaBrasíliaBrazil

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