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European Journal of Plant Pathology

, Volume 152, Issue 2, pp 525–530 | Cite as

Susceptible and tolerant potato leaf-responses post challenge with Pectobacterium carotovorum subsp. brasiliense 1692

  • G.K.E Mosina
  • Lucy Novungayo Moleleki
Article

Abstract

Leaf responses of a susceptible potato cultivar (S. tuberosum cv. Valor) were compared to those of a tolerant potato cultivar (S. tuberosum cv. BP1) following challenge by Pectobacterium carotovorum subsp. brasiliense 1692 (Pcb 1692). Leaves of the susceptible cultivar showed excessive maceration and water soaking symptoms as well as increased proliferation of the bacteria. In leaves of the tolerant cultivar, bacteria appeared to be restricted to the point of inoculation,subsequently; there was very little multiplication of bacterial cells. Furthermore we demonstrated that disease is associated with the extensive spread of cell death in S. tuberosum cv. Valor while in S. tuberosum cv. BP1 cell death was observed to be associated with lack of disease development. Another response associated with challenge by Pcb 1692 in the susceptible and tolerant potato cultivar leaf tissue was the oxidative burst. Generally, the accumulation of hydrogen peroxide and superoxide in leaf-tissue appeared to correlate with tolerance levels.

Keywords

Cell death Pectobacterium Potato Oxidative burst 

Notes

Acknowledgements

This research study was funded by the National Research Foundation (NRF), South Africa through Competitive Funding for Rated Researchers (CFRR) 98993, Research Technology and Transfer Fund (RTF) 98654. GM was funded by the NRF Grant Holder Linked Bursary.

Compliance with ethical standards

1) This material has not been published in whole or in part elsewhere;

2) The manuscript is not currently being considered for publication in another journal;

3) All authors have been personally and actively involved in substantive work leading to the manuscript, and will hold themselves jointly and individually responsible for its content.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Ahn, I. P. (2007). Disturbance of the Ca2+/calmodulin-dependent signalling pathway is responsible for the resistance of Arabidopsis dnd1 against Pectobacterium carotovorum infection. Molecular Plant Pathology, 8(6), 747–759.CrossRefPubMedGoogle Scholar
  2. Buonaurio, R. (2008). Infection and plant defense responses during plant-bacterial interaction. Plant-microbe interactions, 169–197.Google Scholar
  3. Charkowski, A., Blanco, C., Condemine, G., Expert, D., Franza, T., Hayes, C., Hugouvieux-Cotte-Pattat, N., Solanilla, E. L., Low, D., & Moleleki, L. (2012). The role of secretion systems and small molecules in soft-rot enterobacteriaceae pathogenicity. Annual Review of Phytopathology, 50, 425–449.CrossRefPubMedGoogle Scholar
  4. Chisholm, S. T., Coaker, G., Day, B., & Staskawicz, B. J. (2006). Host-microbe interactions: Shaping the evolution of the plant immune response. Cell, 124, 803–814.CrossRefPubMedGoogle Scholar
  5. Davidsson, P. R., Kariola, T., Niemi, O., & Palva, E. T. (2014). Pathogenicity of and plant immunity to soft rot pectobacteria. Induced plant responses to microbes and insects, 122.Google Scholar
  6. De Boer, S., Li, X., & Ward, L. (2012). Pectobacterium spp. associated with bacterial stem rot syndrome of potato in Canada. Phytopathology, 102, 937–947.CrossRefPubMedGoogle Scholar
  7. Glazebrook, J. (2005). Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Review of Phytopathology, 43, 205–227.CrossRefPubMedGoogle Scholar
  8. Hogan, C. S., Mole, B. M., Grant, S. R., Willis, D. K., & Charkowski, A. O. (2013). The type III secreted effector DspE is required early in Solanum tuberosum leaf infection by Pectobacterium carotovorum to cause cell death, and requires Wx (3–6) D/E motifs. PLoS One, 8, e65534.CrossRefPubMedPubMedCentralGoogle Scholar
  9. Kim, H.-S., Ma, B., Perna, N. T., & Charkowski, A. O. (2009). Phylogeny and virulence of naturally occurring type III secretion system-deficient Pectobacterium strains.. Applied and Environmental Microbiology, 75, 4539–4549.CrossRefPubMedPubMedCentralGoogle Scholar
  10. Kim, H.-S., Thammarat, P., Lommel, S. A., Hogan, C. S., & Charkowski, A. O. (2011). Pectobacterium carotovorum elicits plant cell death with DspE/F, but does not suppress callose or induce expression of plant genes early in plant-microbe interactions. Molecular Plant-Microbe Interactions, 24, 773–786.CrossRefPubMedGoogle Scholar
  11. Kubheka, G. C., Coutinho, T. A., Moleleki, N., & Moleleki, L. N. (2013). Colonization patterns of a mCherry-tagged Pectobacterium carotovorum subsp. brasiliense strain in potato plants. Phytopathology, 103(12), 1268–1279.CrossRefPubMedGoogle Scholar
  12. Kwenda, S., Motlolometsi, T. V., Birch, P., & Moleleki, L. N. (2016). RNA-seq profiling reveals defense responses in a tolerant potato cultivar to stem infection by Pectobacterium carotovorum ssp. brasiliense. Frontiers in plant Science, 7, 1905.CrossRefPubMedPubMedCentralGoogle Scholar
  13. Mattinen, L., Tshuikina, M., Mäe, A., & Pirhonen, M. (2004). Identification and characterization of nip, necrosis-inducing virulence protein of Erwinia carotovora subsp. carotovora. Molecular Plant-Microbe Interactions, 17(12), 1366–1375.CrossRefPubMedGoogle Scholar
  14. van der Merwe, J. J., Coutinho, T. A., Korsten, L., & van der Waals, J. E. (2010). Pectobacterium carotovorum subsp. brasiliensis causing blackleg on potatoes in South Africa. European Journal of Plant Pathology, 126, 175–185.CrossRefGoogle Scholar
  15. Moleleki, L. N., Onkendi, E. M., Mongae, A., & Kubheka, G. C. (2012). Characterisation of Pectobacterium wasabie causing blackleg and soft rot diseases in South Africa. European Journal of Plant Pathology., 135, 279–288.CrossRefGoogle Scholar
  16. Onkendi, E. M., & Moleleki, L. N. (2014). Characterization of Pectobacterium carotovorum subsp. carotovorum and brasiliense from diseased potatoes in Kenya. European Journal of Plant Pathology, 139(3), 557–566.CrossRefGoogle Scholar
  17. Pérombelon, M. (2002). Potato diseases caused by soft rot erwinias: An overview of pathogenesis. Plant Pathology, 51, 1–12.CrossRefGoogle Scholar
  18. Shlezinger, N., Minz, A., Gur, Y., Hatam, I., Dagdas, Y. F., Talbot, N. J., & Sharon, A. (2011). Anti-apoptotic machinery protects the necrotrophic fungus Botrytis cinerea from host-induced apoptotic-like cell death during plant infection. PLoS Pathogen, 7(8), e1002185.CrossRefGoogle Scholar
  19. Wolgemuth, H., Mittelstrass, K., Kschieschan, S., Bender, J., Weigel, H.-J., Overmyer, K., Kanasjärvi, J., Sandermann, H., & Langebartels, C. (2002). Activation of an oxidative burst is a general feature of sensitive plants exposed to the air pollutant ozone. Plant, Cell and Environment, 25, 717–726.CrossRefGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  1. 1.Department of Microbiology and Plant PathologyUniversity of PretoriaPretoriaSouth Africa
  2. 2.Forestry, Agriculture and Biotechnology InstituteUniversity of PretoriaPretoriaSouth Africa

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