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Ethiopian barley landraces: useful resistant sources to manage Barley yellow dwarf and other foliar diseases constraining productivity

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Abstract

One hundred sixty-five advanced lines were subjected to marker-assisted selection using Cleaved amplified polymorphic sequence-polymerase chain reaction (CAPS-PCR) primer against Barley yellow dwarf (BYD) resistant Yd2 gene. In addition, phenotypic evaluation was employed to screen the advanced lines for multiple disease resistance. Effect of host growth rate and incubation period on BYD development was also assessed. Results of marker PCR showed that Yd2 gene was detected in 96 (58.2%) out of 165 accessions tested. Data on relationships of days to heading (DtH) and BYD symptoms development revealed that barley accessions having shorter DtH (66 days), BYD severity appeared low, and vice versa on accessions having longer DtH (79–83 days). Screening for multiple disease resistance gave interesting results. Among 11 accessions (with Yd2) selected as the best for multiple diseases, 6 accessions [Chelia local, 4304–2(2), 4818(1.3), 1822–1(1), 1831–2-2(2), and 4915–1(1.2)] were highly resistant to leaf rust and net blotch, while other five lines had moderately resistant reaction. Study on the relationship of host resistance and incubation period showed that BYDV was detectable 5 days earlier in susceptible than in resistant lines. This can be used as an alternative method in preliminary screening of large number of genotypes against BYDV. From this study, it was evident that the identified resistant genotypes are a good resource for barley improvement program targeting BYDV and other important foliar pathogens. Barley breeders are encouraged to take advantage of this opportunity in producing multi-disease resistant barley genotypes in an agronomically enhanced genetic background in the country and beyond.

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Acknowledgments

We thank ICARDA and Holetta Agricultural Biotechnology Centre (Ethiopia) for allowing us to use the Virology and Molecular Biology Laboratories. Special thanks go to Professor Tesfaye Mengiste, Purdue University, USA for kindly providing Markers and DNA extraction kits for laboratory analysis done in Ethiopia. The authors thank Ms. Shaimaa Mahmoud and Nourhan Fouad at ICARDA’s Biotechnology Laboratory, Cairo, Egypt for excellent technical support. We are grateful to Ethiopian Biodiversity Institute (EBI) and Holetta Agricultural Research Centre for providing barley genotypes.

Funding

Field and greenhouse screening work was supported by the Ethiopian Institute of Agricultural Research (EIAR) and East African Agricultural Productivity Project (EAAPP). Molecular laboratory work was supported by ICARDA through Austria Development Agency funded project (Reference No. 2012/05).

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Authors and Affiliations

  1. Ethiopian Institute of Agricultural Research, Ambo Plant Protection Research Centre, Ambo, Ethiopia

    Berhanu Bekele

  2. Haramaya University, Dire Dawa, Ethiopia

    Berhanu Bekele, Chemeda Fininsa & Abdurazak Yusuf

  3. Addis Ababa Science and Technology University, Addis Ababa, Ethiopia

    Adane Abraham

  4. International Centre for Agricultural Research in the Dry Areas (ICARDA), Terbol Station, Zahla, Lebanon

    Safaa G. Kumari

  5. ICARDA, Rabat, Morocco

    Seid Ahmed

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  1. Berhanu Bekele
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  2. Adane Abraham
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  3. Safaa G. Kumari
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  6. Abdurazak Yusuf
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Correspondence to Safaa G. Kumari.

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Bekele, B., Abraham, A., Kumari, S.G. et al. Ethiopian barley landraces: useful resistant sources to manage Barley yellow dwarf and other foliar diseases constraining productivity. Eur J Plant Pathol 154, 873–886 (2019). https://doi.org/10.1007/s10658-018-01644-4

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