Genetic diversity and structure of Fusarium oxysporum f.sp. lentis isolates from Iran, Syria and Algeria

Abstract

Lentil is an important cool season food legume. Vascular wilt incited by Fusarium oxysporum f.sp. lentis (FOL) is the most important disease of lentil worldwide. Knowledge of pathogen genetic structure is crucial to develop effective control strategies. In this study, the genetic structure of a collection of FOL isolates from Iran, Syria and Algeria was analysed using SSR markers. Eight markers were developed in this study and constitute a valuable resource for future molecular studies in FOL. Our results showed that there is a high molecular variation within regions, with isolates from North West Iran showing the highest gene diversity. Variation among regions also exists, with Iranian populations differing significantly from non-Iranian ones, having some private alleles. The clustering of isolates was well in agreement in both distance-based and model-based approaches suggesting the presence of seven ancestral FOL lineages, being three present exclusively in Iran while the others were distributed across all the regions. These results suggest that Iran could be a regional center of origin for FOL.

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Acknowledgments

This research was carried out based on PhD educational mission No. 3972/200-28/1/1389 of first author from Agricultural Research, Education and Extension Organization (AREEO) of Iran and financially supported by Project AGL2014-52871-R co-financed by FEDER. The authors wish to tanks Professor B. Bayaa, University of Aleppo, Syria, for helping to obtain Algerian isolates of FOL.

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Correspondence to Hamid R. Pouralibaba.

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Pouralibaba, H.R., Šatović, Z., Cobos, M.J. et al. Genetic diversity and structure of Fusarium oxysporum f.sp. lentis isolates from Iran, Syria and Algeria. Eur J Plant Pathol 153, 1019–1029 (2019). https://doi.org/10.1007/s10658-018-01617-7

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Keywords

  • Lentil (Lens culinaris)
  • Fusarium oxysporum f.sp. lentis
  • Population structure
  • Pathotype
  • SSR