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Pathological studies on the southern blight of China aster (Callistephus chinensis) caused by Sclerotium rolfsii

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Abstract

A severe outbreak of southern blight disease of China aster was observed during the post rainy season (September–November 2015) in the Mysore and Mandya Districts of Karnataka, Southern India. The disease incidence ranged between 12 and 47%. The typical disease symptoms include water-soaked lesions on leaves, stems and on the lower stem surfaces followed by quick wilting of the whole plant with abundant production of sclerotia near the stem-soil interface. The associated fungal pathogen was isolated on potato dextrose agar (PDA) medium, on which numerous reddish-brown sclerotia were seen. A total of 26 fungal isolates were isolated and studied for the mycelial compatibility. Isolate SrCCM 1 was used for pathogenicity analysis. The results of the study showed that, there was no variation among the isolates tested. Molecular identification of the pathogen by ITS-rDNA sequences of S. rolfsii showed 100% similarity with reference sequences. Based on the cultural, morphological and molecular characteristics, the fungal pathogen was identified as Sclerotium rolfsii Sacc. (Sexual morph: Athelia rolfsii (Curzi) C.C. Tu & Kimbr). Pathogenicity tests were performed on healthy leaves, roots and stems. Typical disease symptoms on leaves, stems and roots were evident after 5, 8 and 10 days of post-inoculation. Sclerotium rolfsii is known to cause diseases in economically important crop plants. However, no reports are available on the occurrence of S. rolfsii on China aster in India.

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Acknowledgements

First author (MKS) acknowledges the Department of Science and Technology (DST), Government of India, New Delhi for providing INSPIRE Fellowship (IF110422). We thank Professor K. R. Sridhar, UGC-BSR Faculty, Mangalore University, Mangalore, India for their help and critical comments.

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Authors and Affiliations

  1. Mycology and Phytopathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore, Karnataka, 570 006, India

    S. Mahadevakumar, C. Chandana, Y. S. Deepika, K. S. Sumashri & G. R. Janardhana

  2. Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore, Karnataka, 570 006, India

    Vandana Yadav

Authors
  1. S. Mahadevakumar
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  2. C. Chandana
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  3. Y. S. Deepika
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  4. K. S. Sumashri
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  5. Vandana Yadav
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  6. G. R. Janardhana
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Correspondence to G. R. Janardhana.

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Mahadevakumar, S., Chandana, C., Deepika, Y.S. et al. Pathological studies on the southern blight of China aster (Callistephus chinensis) caused by Sclerotium rolfsii. Eur J Plant Pathol 151, 1081–1087 (2018). https://doi.org/10.1007/s10658-017-1415-2

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  • DOI: https://doi.org/10.1007/s10658-017-1415-2

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