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European Journal of Plant Pathology

, Volume 150, Issue 3, pp 785–789 | Cite as

Molecular identification of Tomato spotted wilt virus on pepper and tobacco in Republic of Srpska (Bosnia and Herzegovina)

  • Duška DelićEmail author
  • Bachir Balech
  • Mariana Radulović
  • Zorica Đurić
  • Biljana Lolić
  • Monica Santamaria
  • Gordana Đurić
Article

Abstract

Surveys for TSWV presence and distribution on vegetable, ornamental and tobacco plants were conducted during 2016 in open fields and greenhouses in different locations in Republic of Srpska, district of Bosnia and Herzegovina. One hundred and twelve samples were taken and preliminarily tested for TSWV presence with DAS-ELISA. Positive samples were double-checked in RT-PCR assays using specific primer sets and subsequently partial sequences of RdRp and N region genes were characterized. Serological and molecular results revealed twenty-seven TSWV positive samples (two peppers and twenty-five tobacco plants). Moreover, phylogenetic analyses showed that these isolates share the same gene pool and a similar evolutionary pattern mainly with other isolates present in Europe and Russia.

Keywords

TSWV Capsicum annuum Nicotiana tabacum DAS ELISA PCR Sequencing Phylogeny 

Notes

Acknowledgements

This work was supported by the Ministry of Agriculture, Forestry and Water management of Republic of Srpska (contract number 10/3526/16).

Compliance with ethical standards

Hereby we confirm and declare that in the work done and present in this paper there is no any potential conflict of interest, also in the research any human and/or animals participant wasn’t used and there is no any disagreement with informed consent.

Supplementary material

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Fig. S1 (DOC 4420 kb)
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Fig. S2 (DOC 5857 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017

Authors and Affiliations

  1. 1.Faculty of AgricultureUniversity of BanjalukaBanjalukaBosnia and Herzegovina
  2. 2.Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (CNR)BariItaly

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