Skip to main content
SpringerLink
Log in

Molecular identification of Tomato spotted wilt virus on pepper and tobacco in Republic of Srpska (Bosnia and Herzegovina)

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Surveys for TSWV presence and distribution on vegetable, ornamental and tobacco plants were conducted during 2016 in open fields and greenhouses in different locations in Republic of Srpska, district of Bosnia and Herzegovina. One hundred and twelve samples were taken and preliminarily tested for TSWV presence with DAS-ELISA. Positive samples were double-checked in RT-PCR assays using specific primer sets and subsequently partial sequences of RdRp and N region genes were characterized. Serological and molecular results revealed twenty-seven TSWV positive samples (two peppers and twenty-five tobacco plants). Moreover, phylogenetic analyses showed that these isolates share the same gene pool and a similar evolutionary pattern mainly with other isolates present in Europe and Russia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Adkins, S. (2000). Tomato spotted wilt virus-positive steps towards negative success. Molecular Plant Pathology, 1, 151–157.

    Article  CAS  PubMed  Google Scholar 

  • Balech, B., Vicario, S., Donvito, G., Monaco, A., Notarangelo, P., & Pesole, G. (2015). MSA-PAD: DNA multiple sequence alignment framework based on PFAM accessed domain information. Bioinformatics, 31(15), 2571–2573.

    Article  CAS  PubMed  Google Scholar 

  • Chatzivassiliou, E. K., Efthimiou, K., Drossos, E., Papadopoulou, A., Poimenidis, G., & Katis, N. I. (2004). A survey of tobacco viruses in tobacco crops and native flora in Greece. European Journal of Plant Pathology, 110, 1011–1023.

    Article  Google Scholar 

  • Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: More models, new heuristics and parallel computing. Nature Methods, 9(8), 772.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Đurić, Z, Lolić, B, Delić D (2017). Morphological and molecular identification of Frankliniella occidentalis (Pergande) in republic of Srpska (p. 72). Banja Luka: 6th international symposium on agricultural sciences, February 27 – March 2, 2017.

  • EFSA Panel on Plant Health (PLH). (2012). Scientific opinion on the pest categorization of the tospoviruses. EFSA Journal, 10, 3029.

    Article  Google Scholar 

  • Ewing, B., & Green, P. (1998). Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Research, 8, 186–194.

    Article  CAS  PubMed  Google Scholar 

  • Ewing, B., Hillier, L., Wendl, M. C., & Green, P. (1998). Basecalling of automated sequencer traces using phred. I. Accuracy assessment. Genome Research, 8(3), 175–185.

    Article  CAS  PubMed  Google Scholar 

  • Feng, Z., Chen, X., Bao, Y., Dong, J., Zhang, Z., & Tao, X. (2013). Nucleocapsid of Tomato spotted wilt tospovirusforms mobile particles that traffic on an actin/endoplasmic reticulum network driven by myosin XI-K. New Phytologist, 200, 1212–1224.

    Article  CAS  PubMed  Google Scholar 

  • Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W., & Gascuel, O. (2010). New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology, 59(3), 307–321.

    Article  CAS  PubMed  Google Scholar 

  • de Haan, P., Kormelink, R., Oliveira, R., Poelwijk, F., & Peters, D. (1991). Tomatospotted wilt virus L RNA encodes a putative RNA polymerase. Journal of General Virology, 72, 2207–2216.

    Article  PubMed  Google Scholar 

  • Hristova, D., Karadjova, O., Yankulova, M., Heinze, C., & Adam, G. (2001). A survey of tospoviruses in Bulgaria. Journal of Phytopathology, 149, 745–749.

    Article  Google Scholar 

  • Kajić, V. and Milanović J. (2013). Virus pjegavosti i venuća rajčice (Tomato spotted wilt virus – TSWV) i virus nekrotične pjegavosti vodenike (Impatiens necrotic spot virus – INSV)– biljni virusi koji prijete proizvodnji povrća, ukrasnog i industrijskog bilja. Zagreb: Hrvatski centar za poljoprivredu, hranu i selo.

  • Kohnić, A., Ostojić, I., & Karić, N. (2006). Vegetable pests in greenhouses in territory of Herzegovina. Radovi Poljoprivrednog Fakulteta Univerziteta u Sarajevu, 51(2), 139–140.

    Google Scholar 

  • Letunic, I., & Bork, P. (2016). Interactive tree of life (iTOL) v3: An online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Research, 44(W1), W242–W245. doi:10.1093/nar/gkw290.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lian, S., Lee, J.-S., Cho, W. K., Yu, J., Kim, M.-K., Cho, H.-S., & Kim, K.-H. (2013). Phylogenetic and recombination analysis of tomato spotted wilt virus. PloS One, 8(5), e63380. doi:10.1371/journal.pone.0063380.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Margaria, P., Bosco, L., Vallino, M., Ciuffo, M., Mautino, G. C., Tavella, L., & Turina, M. (2014). The NSS protein of Tomato spotted wilt virus is required for persistent infection and transmission by Frankliniella occidentalis. Journal of Virology, 88(10), 5788–5802.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Margaria, P., Ciuffo, M., Rosa, C., & Turina, M. (2015). Evidence of a tomato spotted wilt virus resistance breaking strain originated through natural reassortment between two evolutionary-distinct isolates. Virus Reseerch, 196, 157–161.

    Article  CAS  Google Scholar 

  • Mumford, R. A., Barker, I., & Wood, K. R. (1994). The detection of tomato spotted wilt virus using the polymerase chainreaction. Journal of Virological Methods, 46, 303–311.

    Article  CAS  PubMed  Google Scholar 

  • Nikolić, D., Stanković, I., Vučurović, A., Ristić, D., Milojević, K., Bulajić, A., & Krstić, B. (2013). First report of tomato spotted wilt virus on Brugmansia sp. in Serbia. Plant Disease, 9(6), 850.

    Article  Google Scholar 

  • Pappu, H. R., Csinos, A. S., McPherson, R. M., Jones, D. C., & Stephenson, M. G. (2000). Effect of acibenzolar-S-methyl and imidacloprid on suppression of tomato spotted wilt Tospovirus in flue-cured tobacco. Crop Protection, 19, 349–354.

    Article  CAS  Google Scholar 

  • Škorić, D., Krajačić, M., & Šarić, A. (1997). Tomato spotted wilt Tospovitus isolated from pepper plants in Dalmatia. Petria – Giornale di Patologia delle Piante, 7(1), 47–50.

    Google Scholar 

  • Snippe, M., Willem, B. J., Goldbach, R., & Kormelink, R. (2007). Tomato spotted wilt virus Gc and N proteins interact in vivo. Virology, 357, 115–123.

    Article  CAS  PubMed  Google Scholar 

  • Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Berenji, J., & Krstić, B. (2011a). Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. Acta Virologica, 55, 337–347.

    Article  PubMed  Google Scholar 

  • Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Jović, J., & Krstić, B. (2011b). First report of Tomato spotted wilt virus on Gerbera hybrida in Serbia. Plant Disease, 95(2), 226.

    Article  Google Scholar 

  • Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Nikolić, D., & Krstić, B. (2012). First report of Tomato spotted wilt virus infecting onion and garlic in Serbia. Plant Disease, 96(6), 918.

    Article  Google Scholar 

  • Stanković, I., Bulajić, A., Vučurović., A, Ristić, D., Milojević, K., Nikolić, D., & Krstić, B. (2013) First report of tomato spotted wilt virus on chrysanthemum in Serbia. Plant Disease 97(1), 150–151.

  • Tentchev, D., Verdin, E., Marchal, C., Jacquet, M., Aguilar, J. M., & Moury, B. (2011). Evolution and structureof Tomato spotted wilt virus populations: Evidence of extensive reassortment and insights into emergence processes. Journal of General Virology, 92, 961–973.

    Article  CAS  PubMed  Google Scholar 

  • Trkulja, V., Mihić Salapura, J., Ćurković, B., Stanković, I., Bulajić, A., Vučurović, A., & Krstić, B. (2013). First report of tomato spotted wilt virus on gloxinia in Bosnia and Herzegovina. Plant Disease, 97, 429.

    Article  Google Scholar 

  • Tsompana, M., Abad, J., Purugganan, M., & Moyer, J. W. (2005). The molecular population genetics of the Tomato spottedwilt virus (TSWV) genome. Molecular Ecology, 14, 53–66.

    Article  CAS  PubMed  Google Scholar 

  • Turina, M., Tavella, L., & Ciuffo, M. (2012). Tospoviruses in the Mediterranean area. Advances in Virus Research, 84, 403–437.

    Article  PubMed  Google Scholar 

  • Turina, M., Kormelink, R., & Resende, O. (2016). Resistance to Tospoviruses in vegetable crops: Epidemiological and molecular aspects. Annual Review of Phytopathology, 54, 347–371.

    Article  CAS  PubMed  Google Scholar 

  • Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J., & Krstić, B. (2012). Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of zucchini yellow mosaic virus isolates. European Journal of Plant Pathology, 133, 935–947.

    Article  Google Scholar 

  • Webster, C. G., Frantz, G., Reitz, S. R., Funderburk, J. E., Mellinger, H. C., Turechek, W. W., Marshall, S. H., Tantiwanich, Y., McGrath, M. T., Daughtrey, M. L., & Adkins, S. (2015). Emergence of Groundnutringspot virus and Tomato chlorotic spot virus in vegetables in Florida and the southeastern United States. Phytopathology, 105, 388–398.

    Article  CAS  PubMed  Google Scholar 

  • Zhang, Z., Wang, D., Yu, C., Wang, Z., Dong, J., Shi, K., & Yuan, X. (2016). Identification of three new isolates of tomato spotted wilt virus from different hosts in China: Molecular diversity, phylogenetic and recombination analyses. Virology Journal, 13(8), 1–12.

    Google Scholar 

  • Zindović, J., Bulajić, A., Krstić, B., Ciuffo, M., Margaria, P., & Turina, M. (2011). First report of tomato spotted wilt virus on pepper in Montenegro. Plant Disease, 95(7), 882.

    Article  Google Scholar 

  • Zindović, J., Ciuffo, M., & Turina, M. (2014). Molecular characterization of tomato spotted wilt virus in Montenegro. Journal of Plant Pathology, 96(1), 201–205.

    Google Scholar 

Download references

Acknowledgements

This work was supported by the Ministry of Agriculture, Forestry and Water management of Republic of Srpska (contract number 10/3526/16).

Author information

Author notes

  1. Duška Delić and Bachir Balech contributed equally to this work.

Authors and Affiliations

  1. Faculty of Agriculture, University of Banjaluka, Bulevar vojvode Petra Bojovića 1A, 78000, Banjaluka, Bosnia and Herzegovina

    Duška Delić, Mariana Radulović, Zorica Đurić, Biljana Lolić & Gordana Đurić

  2. Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (CNR), via Amendola 165/A, 70126, Bari, Italy

    Bachir Balech & Monica Santamaria

Authors
  1. Duška Delić
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bachir Balech
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mariana Radulović
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zorica Đurić
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Biljana Lolić
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Monica Santamaria
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gordana Đurić
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Duška Delić.

Ethics declarations

Hereby we confirm and declare that in the work done and present in this paper there is no any potential conflict of interest, also in the research any human and/or animals participant wasn’t used and there is no any disagreement with informed consent.

Electronic supplementary material

Fig. S1

(DOC 4420 kb)

Fig. S2

(DOC 5857 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Delić, D., Balech, B., Radulović, M. et al. Molecular identification of Tomato spotted wilt virus on pepper and tobacco in Republic of Srpska (Bosnia and Herzegovina). Eur J Plant Pathol 150, 785–789 (2018). https://doi.org/10.1007/s10658-017-1313-7

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-017-1313-7

Keywords

Search

Navigation