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Genetic structure of Aphanomyces euteiches populations sampled from United States and France pea nurseries

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Abstract

Aphanomyces euteiches Drechsler is an oomycete pathogen of leguminous crops that causes root rot, a severe disease of pea (Pisum sativum L.) worldwide. An improved understanding of the genetic structure of A. euteiches populations would increase knowledge of pathogen evolution and assist in the design of strategies to develop pea cultivars and germplasm with stable disease resistance. Twenty six primers pairs were used to amplify Sequence Related Amplified Polymorphisms (SRAP) among 49 A. euteiches isolates sampled from pea. A total of 190 polymorphic SRAP bands were generated, of which 82 were polymorphic between all the A. euteiches isolates. The percentage of polymorphic bands per primer pair ranged from 22 to 75%. According to the PIC value estimated for each marker, 60% of the SRAP markers were highly to reasonably informative (PIC > 0.25). Genetic structure of A. euteiches populations sampled in different American and French locations showed low to high genetic diversity within populations. The largest variation occurred within countries, with a total estimated genetic diversity of 0.477 and 0.172 for American and French populations, respectively. This was particularly evident from a principal component analysis (PCA) and a Minimum Spanning Networks (MSN) based on genetic profiles of isolates, which generated two different clusters, one corresponding to the French isolates and four American isolates (MV1, MV5, MV7, Ath3), and the other to American isolates. A. euteiches populations from cultivated pea in France appeared as a single unstructured population, whereas American isolates of A. euteiches diverged into three different populations.

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Acknowledgements

The authors would like to thank Emmanuel Wicker (CIRAD, La Réunion), and Hélène Magalon (Laboratoire d’écologie marine, Université de la Réunion) for their valuable advice in the phylogenetic analyses. Financial support through grants of the Plant Health and the Environment Division of INRA, Terres Univia, AGROCAMPUS Ouest, and the US Department of Agriculture (USDA), is gratefully acknowledged. The authors would like to thank Dr. C. Coyne (USDA-ARS, Pullman) for providing soils from US.

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Authors and Affiliations

  1. AGROCAMPUS Ouest, Laboratoire ESP (Ecologie et Santé des Plantes), 65 rue de Saint Brieuc, 35042, Rennes, France

    C. Le May

  2. INRA, UMR 1349 IGEPP Institute de Génétique, Environnement et Protection des Plantes, Domaine de la Motte, BP 35327, 35653, Le Rheu, France

    C. Le May, C. Onfroy, A. Moussart, D. Andrivon, A. Baranger & M. L. Pilet-Nayel

  3. PISOM, UMT INRA/Terres Inovia, UMR IGEPP 1349, Domaine de la Motte au Vicomte, BP 35327, 35653, Le Rheu cedex, France

    C. Le May, C. Onfroy, A. Moussart, A. Baranger & M. L. Pilet-Nayel

  4. Terres Inovia, 11 rue de Monceau, CS 60003, 75378, Paris cedex 08, France

    C. Onfroy & A. Moussart

  5. Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Pullman, WA, 99164, USA

    G. Vandemark

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The research presented in this manuscript does not implicate nor Human Participants and/or Animals.

CLM and GV were the principal investigator of this research project. They conducted the research experiments and contributed to the data analysis and to drafting the manuscript. CO contributed to the A. euteiches strains baiting, and to the drafting of the manuscript. MLP, AB, AM and DA contributed to the drafting of the manuscript. All authors read and approved the manuscript.

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Le May, C., Onfroy, C., Moussart, A. et al. Genetic structure of Aphanomyces euteiches populations sampled from United States and France pea nurseries. Eur J Plant Pathol 150, 275–286 (2018). https://doi.org/10.1007/s10658-017-1274-x

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