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Interaction of root colonizing biocontrol agents demonstrates the antagonistic effect against Fusarium oxysporum f. sp. lycopersici on tomato

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Abstract

Plant growth promoting Bacillus subtilis MSS9 and Bacillus licheniformis MSS14 were isolated from the tomato rhizosphere. These isolates were capable of inhibiting the fungal pathogen, Fusarium oxysporum f. sp. lycopersici causing fusarium wilt in tomato, tested by dual culture method and by mycolytic enzyme production. The isolates have the capacity to form biofilm on the microtitre plate. Scanning electron microscopy revealed good colonization capacity of Bacillus licheniformis MSS14 on tomato plant root as compared to Bacillus subtilis MSS9, pot experiments were also analyzed to study the effects of both rhizobacterial cultures on pathogen development and plant growth. It was observed that MSS14 reduces the incidence of Fusarium oxysporum f. sp. lycopersici in tomato and there was significant increase in vegetative parameters like root length, shoot length, plant wet weight, dry weight and chlorophyll content after which indicates that the root colonization property of the culture MSS14 helps in enhancing the biocontrol capacity against pathogen than that of MSS9.

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Acknowledgements

We are thankful to Department of Microbiology and Biotechnology, Gujarat University to encourage and help us with the required facility and work and British Petroleum International Pvt. Ltd. for financial support.

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  1. Department of Microbiology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, 380 009, India

    Stuti Patel & Meenu Saraf

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  1. Stuti Patel
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  2. Meenu Saraf
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Correspondence to Meenu Saraf.

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Patel, S., Saraf, M. Interaction of root colonizing biocontrol agents demonstrates the antagonistic effect against Fusarium oxysporum f. sp. lycopersici on tomato. Eur J Plant Pathol 149, 425–433 (2017). https://doi.org/10.1007/s10658-017-1192-y

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