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Evaluation of Greek grapevine cultivars for resistance to Phaeomoniella chlamydospora

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Abstract

In the present study, four Greek (Agiorgitiko, Asyrtiko, Roditis and Xinomavro) and one international (Soultanina) grapevine cultivars (Vitis vinifera L.) were screened for their resistance to Phaeomoniella chlamydospora. Artificial inoculation was carried out by drilling a hole into the trunk and injecting a concentrated conidial suspension into the vessels. Disease reactions were evaluated in an 87-day assessment period, on the basis of external symptoms (disease incidence, disease severity and mortality) and by calculating the relative areas under disease progress curves (relative AUDPC). The extension of vascular browning as well as the isolation ratio along the inoculated vine trunks were also taken into account as additional parameters for evaluating resistance. The results indicated that the resistance of grapevine cultivars to P. chlamydospora varied significantly. ‘Agiorgitiko’ and ‘Soultanina’ were susceptible, whereas ‘Asyrtiko’ and ‘Xinomavro’ were resistant; ‘Roditis’ showed an intermediate level of resistance. Cultivars’ resistance was mostly distinguished in terms of the extension of vascular browning and pathogen isolation ratio. On the contrary, the disease incidence, final disease severity, mortality and relative AUDPC provided less distinctive efficiency in resistance evaluation. The robust methodology presented here could be useful in rapid evaluation experiments for future screening programs to search and recognize natural resistant sources within grapevine genotypes against P. chlamydospora.

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Acknowledgements

This research project was funded under the Action ‘Research & Technology Development Innovation Projects’-AgroETAK, MIS 453350, in the framework of the Operational Program ‘Human Resources Development’, through a post-doctoral grant to Emmanouil A. Markakis. The project was co-funded by the European Social Fund through the National Strategic Reference Framework (Research Funding Program 2007-2013) coordinated by the Hellenic Agricultural Organization – DEMETER (Institute of Olive Tree, Subtropical Crops and Viticulture (Chania, Crete, Greece). Authors also acknowledge Mr. Konstantinos Bakasietas (Bakasietas Vine Nursery, Leontio, Corinthia, Greece) for providing the plant material used in the present study.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization Demeter, Agrokipio 73100, Chania, Crete, Greece

    Emmanouil A. Markakis

  2. Laboratory of Olive Cultivation, Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization Demeter, Agrokipio 73100, Chania, Crete, Greece

    Georgios C. Koubouris & Chrysi K. Sergentani

  3. Laboratory of Plant Pathology, Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Hellenic Agricultural Organization Demeter, Mesa Katsampas 71003, Iraklio, Crete, Greece

    Eleftherios K. Ligoxigakis

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  1. Emmanouil A. Markakis
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  2. Georgios C. Koubouris
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  3. Chrysi K. Sergentani
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  4. Eleftherios K. Ligoxigakis
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Correspondence to Emmanouil A. Markakis.

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Markakis, E.A., Koubouris, G.C., Sergentani, C.K. et al. Evaluation of Greek grapevine cultivars for resistance to Phaeomoniella chlamydospora . Eur J Plant Pathol 149, 277–283 (2017). https://doi.org/10.1007/s10658-017-1186-9

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  • DOI: https://doi.org/10.1007/s10658-017-1186-9

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