European Journal of Plant Pathology

, Volume 148, Issue 4, pp 907–917 | Cite as

Morphological and molecular analysis of Paratrichodorus teres (Hooper 1962) (Nematoda: Trichodoridae): a groundwork for discussion on the phylogeny and pathogenicity of Paratrichodorus species

  • Krassimira Ilieva-Makulec
  • Katarzyna Rybarczyk-Mydłowska
  • Grażyna Winiszewska
  • Łukasz Flis
  • Anna Tereba
  • Katarzyna Kowalewska
  • Tadeusz Malewski
Article

Abstract

Due to its ability to transmit plant viruses, Paratrichodorus teres (Hooper in Nematologica, 7, 273–280, 1962) is recognized as an economically important trichodorid species. Morphological and molecular analyses (18S and 28S rDNA) were performed, and 10 new plant hosts are reported for Polish P. teres populations. Major morphological features and the measurements obtained for the investigated specimens were within the wide ranges indicated for this species. However, a more detailed comparative analysis of Polish and Iranian P. teres showed significant morphological differences, particularly, in the shape and the structure of the walls of pars proximalis vaginae and the shape of the rectum.

Phylogenetic study based on the 18S rDNA data suggests positioning of the Polish P. teres sequences within a cluster of sequences originating from the Netherlands. A comparison of the 28S rDNA fragment from Polish populations with the only P. teres 28S rDNA sequence available (from Iran) in GenBank revealed a sequence variability of 9.3%. The variation across these two representatives was higher than in the case of many other pairs of Trichodoridae species. The results obtained on the Polish P. teres specimens are discussed in the framework of the species taxonomy and phylogenetic relationships.

Keywords

Stubby root nematodes Virus vector nematodes 18S rDNA 28S rDNA Poland Iran 

Notes

Acknowledgements

This research was conducted by the Museum and Institute of Zoology, Polish Academy of Sciences within the project WND-POIG.01.03.01-00-133/09 “Development of innovative methods for rapid identification of nematodes that cause damage to the economy” under the Operational Programme Innovative Economy 2007–2013, which is co-financed by EU funds. We wish to kindly thank Prof. Dr. Wilfrida Decraemer and Dr. Yves Samyn from Royal Belgian Institute of Natural Sciences, Brussels, Belgium for loaning us the specimens of P. teres described by Heydari et al. (2014a) from Iran. We also wish to thank Dr. Johannes Helder from the Wageningen University for making available the photographic documentation of P. teres individuals from the Netherlands and for providing information that sequences of 18S rDNA for that species available in GenBank (FJ040484, KJ636337, KJ636338) were derived from specimens collected in the Netherlands.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017

Authors and Affiliations

  • Krassimira Ilieva-Makulec
    • 1
  • Katarzyna Rybarczyk-Mydłowska
    • 2
  • Grażyna Winiszewska
    • 2
  • Łukasz Flis
    • 2
  • Anna Tereba
    • 2
  • Katarzyna Kowalewska
    • 2
  • Tadeusz Malewski
    • 2
  1. 1.Institute of Ecology and BioethicsKardynał Stefan Wyszyński University in WarsawWarsawPoland
  2. 2.Museum and Institute of Zoology PASWarsawPoland

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