Abstract
Due to its ability to transmit plant viruses, Paratrichodorus teres (Hooper in Nematologica, 7, 273–280, 1962) is recognized as an economically important trichodorid species. Morphological and molecular analyses (18S and 28S rDNA) were performed, and 10 new plant hosts are reported for Polish P. teres populations. Major morphological features and the measurements obtained for the investigated specimens were within the wide ranges indicated for this species. However, a more detailed comparative analysis of Polish and Iranian P. teres showed significant morphological differences, particularly, in the shape and the structure of the walls of pars proximalis vaginae and the shape of the rectum.
Phylogenetic study based on the 18S rDNA data suggests positioning of the Polish P. teres sequences within a cluster of sequences originating from the Netherlands. A comparison of the 28S rDNA fragment from Polish populations with the only P. teres 28S rDNA sequence available (from Iran) in GenBank revealed a sequence variability of 9.3%. The variation across these two representatives was higher than in the case of many other pairs of Trichodoridae species. The results obtained on the Polish P. teres specimens are discussed in the framework of the species taxonomy and phylogenetic relationships.
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References
Aballay, E., & Eriksson, B. (2006). Trichodorid nematodes in the central area of Chile. Nematologia Mediterranea, 34, 43–48.
Bik, H. M., Hawkins, L. E., Hughes, J. A., & Lambshead, J. D. (2009). Rapid decline of PCR amplification from genomic extracts of DESS-preserved, slide-mounted nematodes. Nematology, 11, 827–834.
Boutsika, K., Phillips, M. S., MacFarlane, S. A., Brown, D. J. F., Holeva, R. C., & Blok, V. C. (2003). Molecular diagnostics of trichodorid nematodes and their associated tobacco rattle virus (TRV). Plant Pathology, 53, 110–116.
Decraemer, W. (1995). The family Trichodoridae: stubby root and virus vector nematodes. Dordrecht: Kluwer Academic Publishers 360 pp.
Decraemer, W., Radivojević, M., & De La Peña, E. (2008). Trichodoridae (Nematoda: Triplonchida) from the Tara National Park, Serbia, and proposal of Trichodorus pseudobursatus n.Sp. Nematology, 10, 405–431.
Duarte, I. M., Almeida, M. T. M., Brown, D. J. F., Marques, I., Neilson, R., & Decraemer, W. (2010). Phylogenetic relationships, based on SSU rDNA sequences, among the didelphic genera of the family Trichodoridae from Portugal. Nematology, 12, 171–180.
Duarte, I. M., Almeida, M. T. M., Duarte, M. M., Brown, D. J. F., & Neilson, R. (2011). Molecular diagnosis of trichodorid species from Portugal. Plant Pathology, 60, 586–594.
Fauna Europea. (2013). Fauna Europea subversion 2.6c-22. Web Service available online at http://www.fauna-eu.org.
Hall, T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98; The Find Model web implementation: http://hiv.lanl.gov/content/sequence/findmodel/findmodel.html
Heydari, R., Tanha Maafi, Z., Omati, F., & Decraemer, W. (2014a). First report of Paratrichodorus teres (Diphtherophorina, Trichodoridae) from Iran. Australasian Plant Disease Notes. doi:10.1007/s13314-014-0131-4.
Heydari, R., Tanha Maafi, Z., & Decraemer, W. (2014b). Morphological and molecular characterization of Trichodorus golestanensis (Nematoda: Trichodoridae), a new species from Iran. European Journal of Plant Pathology, 140, 329–340.
Holeva, R., Phillips, M. S., Neilson, R., Brown, D. J. F., Young, V., Boutsika, K., & Blok, V. C. (2006). Real-time PCR detection and quantification of vector trichodorid nematodes and tobacco rattle virus. Molecular and Cellular Probes, 20, 203–211.
Hooper, D. J. (1962). Three new species of Trichodorus (Nematoda: Dorylaimoidea) and observation on T. minor Colbran, 1956. Nematologica, 7, 273–280.
Karanastasi, E., Decraemer, W., Kyriakopoulou, P. E., & Neilson, R. (2005). First report of the stubby-root nematode Paratrichodorus teres associated with artichoke (Cynara scolymus) in Greece. Disease Notes, 89(6), 685.
Koressaar, T., & Remm, M. (2007). Enhancements and modifications of primer design program Primer3. Bioinformatics, 23(10), 1289–1291.
Kuiper, K., & Loof, P. A. A. (1962). Trichodorus flevensis n. Sp. (Nematoda: Enoplida), a plant nematode from new polder soil. Verslagen en Mededelingen van de Plantenziektenkundige Dienst te Wageningen, 136, 193–200.
Kumari, S., & Subbotin, S. A. (2012). Molecular characterization and diagnostics of stubby root and virus vector nematodes of the family Trichodoridae (Nematoda: Triplonchida) using ribosomal RNA genes. Plant Pathology, 61, 1021–1031.
Loof, P. A. A. (1965). Trichodorus anemones n. Sp. with a note on T. teres Hooper, 1962 (Nematoda: Enoplida). Verslagen en Mededelingen van de Plantenziektenkundige Dienst te Wageningen, 142, 132–136.
Loof, P. A. A. (1975). Taxonomy of Trichodoridae. In F. Lamberti, C. E. Taylor, & J. W. Seinhorst (Eds.), Nematode vectors of plant viruses (pp. 103–127). Plenum Press: London & New York.
Miller, M. A., Pfeiffer, W., Schwartz, T. (2010). Creating the CIPRES Science Gateway for Inference of Large Phylogenetic Trees. In SC10 Workshop on Gateway Computing Environments (GCE10).
Ploeg, A. T., & Decraemer, W. (1996). The occurrence and distribution of trichodorid nematodes and their associated tobraviruses in Europe and the former soviet union. Nematologica, 43, 228–251.
Ploeg, A. T., Brown, D. J. F., & Robinson, D. J. (1992). The association between species of Trichodorus and Paratrichodorus vector nematodes and serotypes of tobacco rattle tobravirus. Annals of Applied Biology, 121, 619–630.
Ploeg, A. T., Frans, C. Z., & Maas, P. W. T. (1996). Transmission efficiency of five tobravirus strains by Paratrichodorus teres. European Journal of Plant Pathology, 102, 123–126.
Posada, D., & Crandall, K. A. (1998). MODELTEST: testing the model of DNA substitution. Bioinformatics, 14(9), 817–818.
Rambaut, A., Suchard, M. A., Xie, D., Drummond, A. J. (2014). Tracer v.1.6, available from http://beast.bio.ed.ac.uk/Tracer.
Riga, E., & Neilson, R. (2005). First report of the stubby-root nematode, Paratrichodorus teres, from potato in the Columbia basin of Washington state. Disease notes, 89(12), 1361.
Riga, E., Larsen, R., Eastwell, K., Guerra, N., Guerra, L., & Crosslin, J. M. (2009). Rapid detection of tobacco rattle tobravirus in viruliferous Paratrichodorus allius from greenhouse and field specimens. Journal of Nematology, 41, 60–63.
Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574. doi:10.1093/bioinformatics/btg180.
Seinhorst, J. W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerine. Nematologica, 4, 67–69.
Siddiqi, M. R. (1974). Systematics of the genus Trichodorus Cobb, 1913 (Nematoda: Dorylaimida), with descriptions of three new species. Nematologica, 19, 259–278.
Stamatakis, A. (2014). RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30(9), 1312–1313 doi:10.1093/bioinformatics/btu033.
Taylor, C. E. & Brown, D. J. F. (1997). Nematode vectors of plant viruses. Wallingford, UK, CABI Publishing, 286 pp.
Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B. C., Remm, M., & Rozen, S. G. (2012). Primer3 – new capabilities and interfaces. Nucleic Acids Research, 40(15), e115.
van der Wilk, F., Korsman, M., & Zoon, F. (1994). Detection of tobacco rattle virus in nematodes by reverse transcription and polymerase chain reaction. European Journal of Plant Pathology, 100, 109–122.
van Hoof, H. A. (1962). Trichodorus pachydermus and T. teres, vectors of the early browning virus of peas. Tijdschrift Plantenziekten, 68, 391–396.
van Megen, H., van den Elsen, S., Holterman, M., Karssen, G., Mooyman, P., Bongers, T., Holovachov, O., Bakker, J., & Helder, J. (2009). A phylogenetic tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences. Nematology, 11, 927–950.
Yoder, M., De Ley, I. T., King, I., Mundo-Ocampo, M., Mann, J., Blaxter, M., Poiras, L., & De Ley, P. (2006). DESS: a versatile solution for preserving morphology and extractable DNA of nematodes. Nematology, 8, 367–376.
Acknowledgements
This research was conducted by the Museum and Institute of Zoology, Polish Academy of Sciences within the project WND-POIG.01.03.01-00-133/09 “Development of innovative methods for rapid identification of nematodes that cause damage to the economy” under the Operational Programme Innovative Economy 2007–2013, which is co-financed by EU funds. We wish to kindly thank Prof. Dr. Wilfrida Decraemer and Dr. Yves Samyn from Royal Belgian Institute of Natural Sciences, Brussels, Belgium for loaning us the specimens of P. teres described by Heydari et al. (2014a) from Iran. We also wish to thank Dr. Johannes Helder from the Wageningen University for making available the photographic documentation of P. teres individuals from the Netherlands and for providing information that sequences of 18S rDNA for that species available in GenBank (FJ040484, KJ636337, KJ636338) were derived from specimens collected in the Netherlands.
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Ilieva-Makulec, K., Rybarczyk-Mydłowska, K., Winiszewska, G. et al. Morphological and molecular analysis of Paratrichodorus teres (Hooper 1962) (Nematoda: Trichodoridae): a groundwork for discussion on the phylogeny and pathogenicity of Paratrichodorus species. Eur J Plant Pathol 148, 907–917 (2017). https://doi.org/10.1007/s10658-016-1145-x
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DOI: https://doi.org/10.1007/s10658-016-1145-x