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Molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Costa Rica, with notes on Xiphinema setariae Tarjan, 1964

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Abstract

Pseudomonodelphic dagger nematodes of the genus Xiphinema are characterized by having one of the genital branches reduced and lacking an ovary. They are usually reported from tropical regions. Nematode surveys conducted during rainy seasons in Costa Rica resulted in detection of several Xiphinema costaricense populations, but also other pseudomonodelphic and didelphic species of Xiphinema. We undertook detailed integrative morphometric and molecular studies using D2-D3 expansion segments of 28S rDNA, and ITS1-rDNA. From those studies, we also identified several populations of Xiphinema krugi and two populations of Xiphinema setariae with characteristics in agreement with those of the original and later descriptions of these species. The phylogenetic analyses of these species with other representatives of Xiphinema spp. indicated that pseudomonodelphic species are phylogenetically related (X. costaricense and X. krugi). On the basis of ITS1 sequences of X. costaricense and X. variegatum from Brazil, as well as similar morphology and morphometrics of both species, the latter is proposed here as a junior synonym of the former. Also, our morphometric data showed some intraspecific variability within X. setariae, which in combination with the molecular evidence, suggests that X. setariae and X. vulgare need to be considered as a single taxon.

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References

  • Alkemade, J. R. M., & Loof, P. A. A. (1990). The genus Xiphinema cobb, 1913 (Nematoda: Longidoridae) in Perú. Revue de Nématologie, 13, 339–348.

    Google Scholar 

  • Archidona-Yuste, A., Navas-Cortés, J. A., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., & Castillo, P. (2016). Molecular phylogenetic analysis and comparative morphology resolve two new species of olive-tree soil related dagger nematodes of the genus Xiphinema (Dorylaimida: Longidoridae) from Spain. Invertebrate Systematics, 30, 000–000. doi:10.1071/IS16002.

    Google Scholar 

  • Bala, G. (1984). Occurrence of plant-parasitic nematodes associated with crops of agricultural importance in Trinidad. Nematropica, 14, 37–45.

    Google Scholar 

  • Bala, G., & Rosein, F. (1996). P1ant-parasitic nematodes associated with anthuriums and other tropical ornamentals. Nematropica, 26, 9–14.

    Google Scholar 

  • Barker, K. R. (1985). Nematode extraction and bioassays. In K. R. Barker, C. C. Carter, & J. N. Sasser (Eds.), An advanced treatise on Meloidogyne, Vol. 2, methodology (pp. 19–35). Raleigh: North Carolina State University Graphics.

    Google Scholar 

  • Castillo, P., Vovlas, N., Subbotin, S.A., & Troccoli, A. (2003). A new root-knot nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), parasitizing wild olive in southern Spain. Phytopathology, 93, 1093–1102.

  • Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution, 17, 540–552.

    Article  CAS  PubMed  Google Scholar 

  • Chaves, E. (1984). Observations on plant parasitic nematodes from Argentina. PhD thesis (p. 106). Belgium: University of Ghent.

    Google Scholar 

  • Cherry, T., Szalanski, A. L., Todd, T. C., & Powers, T. O. (1997). The internal transcribed spacer region of Belonolaimus (Nemata: Belonolaimidae). Journal of Nematology, 29, 23–29.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Cohn, E., & Sher, S. A. (1972). A contribution to the taxonomy of the genus Xiphinema cobb, 1913. Journal of Nematology, 4, 36–65.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Coolen, W. A. (1979). Methods for extraction of Meloidogyne spp. and other nematodes from roots and soil. In F. Lamberti & C. E. Taylor (Eds.), Root-knot nematodes (Meloidogyne species). Systematics, biology and control (pp. 317–329). New York: Academic Press.

    Google Scholar 

  • Coomans, A., Huys, R., Heyns, J., & Luc, M. (2001). Character analysis, phylogeny and biogeography of the genus Xiphinema cobb, 1913 (Nematoda: Longidoridae). Annales du Musée Royal de l’Afrique Centrale (Zoologie), Tervuren, Belgique, 287, 1–239.

    Google Scholar 

  • Costa Manso, E. S. B. G., Tenente, R. E. V., Ferraz, L. C. C. B., Oliveira, L. S., & Mesquita, R. (1994). Catálogo de nematóides fitoparasitos encontrados asociados a diferentes tipos de plantas no Brasil (p. 488). Brasília: Mnistério da Agricultura, EMBRAPA/CENARGEN/SPI.

    Google Scholar 

  • Crozzoli, R., Lamberti, F., Greco, N., & Rivas, D. (2001). Plant parasitic nematodes associated with cocoa in Choroni, Cumboto and Cuyagua, Aragua state. Fitopatologia Venezolana, 14, 5–12.

    Google Scholar 

  • De Ley, P., Félix, M. A., Frisse, L. M., Nadler, S. A., Sternberg, P. W., & Thomas, K. W. (1999). Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, 1, 591–612.

    Article  CAS  Google Scholar 

  • De Luca, F., Reyes, A., Grunder, J., Kunz, P., Agostinelli, A., De Giorgi, C., & Lamberti, F. (2004). Characterization and sequence variation in the rDNA region of six nematode species of the genus Longidorus (Nematoda). Journal of Nematology, 36, 147–152.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Decraemer, W., Luc, M., Doucet, M. E., & Coomans, A. (1998). Longidoridae from Argentina with the description of Paraxiphidorus brevistylus sp. n. (Nematoda: Longidoridae). Fundamental and Applied Nematology, 21, 371–388.

    Google Scholar 

  • Doucet, M. E., Ferraz, L. C. C. B., Magunacelaya, J. C., & Brown, D. J. F. (1998). The occurrence and distribution of longidorid nematodes in Latin America. Russian Journal of Nematology, 6, 111–128.

    Google Scholar 

  • Flegg, J. J. M. (1967). Extraction of Xiphinema and Longidorus species from soil by a modification of Cobb’s decanting and sieving technique. Annals of Applied Biology, 60, 429–437.

    Article  Google Scholar 

  • Frederick, J. J., & Tarjan, A. C. (1974). Morphological variation in Xiphinema krugi Lordello, 1955. Proceedings of the Soil and Crop Science Society of Florida, 34, 181–195.

    Google Scholar 

  • Germani, G. (1989). Description of Xiphinema guillaumeti sp. n. (Nematoda: Longidoridae) and measurements on six other species of Xiphinema from Brazilian Amazonia. Nematologica, 35, 69–75.

    Article  Google Scholar 

  • Gutiérrez-Gutiérrez, C., Cantalapiedra-Navarrete, C., Remesal, E., Palomares-Rius, J. E., Navas-Cortés, J. A., & Castillo, P. (2013). New insight into the identification and molecular phylogeny of dagger nematodes of the genus Xiphinema (Nematoda: Longidoridae) with description of two new species. Zoological Journal of the Linnean Society, 169, 548–579.

    Article  Google Scholar 

  • Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98NT. Nucleic Acids Symposium Series, 41, 95–98.

    CAS  Google Scholar 

  • He, Y., Subbotin, S.A., Rubtsova, T. V., Lamberti, F., Brown, D. J. F., & Moens, M. (2005). A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology, 7, 111–124.

    Article  CAS  Google Scholar 

  • Heyns, J. (1977). The genus Xiphinema in South Africa. IV. X. krugi Lordello, 1955, X. mediterraneum Martelli & Lamberti, 1967, and a new species of the X. hallei-group (Nematoda: Dorylaimida). Phytophylactica, 9, 109–114.

    Google Scholar 

  • Hunt, D. J., & Singh, N. D. (1984). Xiphinema costaricense Lamberti & Tarjan, 1974 and X. insigne Loos, 1949, from Trinidad. Revue de Nématologie, 7, 19–22.

    Google Scholar 

  • Hunt, D. J., & Towle, A. (1979). Feeding studies on Xiphinema vulgare Tarjan, 1964. Revue de Nématologie, 2, 37–40.

    Google Scholar 

  • Jairajpuri, M. S., & Ahmad, W. (1992). Dorylaimida. Freeliving, predaceous and plant-parasitic nematodes (p. 458). New Delhi: India, Oxford & IBH Publishing Co..

    Google Scholar 

  • Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780.

  • Kermarrec, A., & Scotto La Massese, C. (1972). Données nouvelles sur la composition et la dynamique de la nematofaune des sols des Antilles francaises. Annales de Zoologie et Ecologie animale, 4, 513–527.

    Google Scholar 

  • Lamberti, F., & Tarjan, A. C. (1974). Xiphinema costaricense n. Sp. (Longidoridae, Nematoda) a new species of dagger nematode from Costa Rica. Nematologia Mediterranea, 2, 1–11.

    Google Scholar 

  • Lamberti, F., Jatala, P., & Agostinelli, A. (1987). A report of some Xiphinema species occurring in Peru. Nematologia Mediterranea, 15, 103–109.

    Google Scholar 

  • Lamberti, F., D’Addabbo, T., Arias, M., Agostinelli, A., & Bravo, M. A. (1995). On the synonymy of Xiphinema vulgare Tarjan, 1964 with X. setariae Luc, 1958 (Nematoda, Dorylaimida). Nematologia Mediterranea, 23, 131–145.

    Google Scholar 

  • Loof, P. A. A., & Luc, M. (1990). A revised polytomous key for the identification of species of the genus Xiphinema cobb, 1913 (Nematoda: Longidoridae) with exclusion of the X. americanum-group. Systematic Parasitology, 16, 35–66.

    Article  Google Scholar 

  • Loof, P. A. A., & Maas, P. W. T. (1972). The genus Xiphinema (Dorylaimida) in Surinam. Nematologica, 18, 91–119.

    Google Scholar 

  • Loof, P. A. A., & Sharma, R. D. (1979). Plant parasitic nematodes from Bahia State, Brazil: The genus Xiphinema Cobb, 1913 (Dorylaimoidea). Nematologica 25, 111–127.

  • Lordello, L. G. E. (1955). Xiphinema krugi n. Sp (Nematoda: Dorylaimidae) from Brazil with a key to the species of Xiphinema. Proceedings of the Helminthological Society of Washington, 22, 16–21.

    Google Scholar 

  • Luc, M. (1958). Xiphinema of West Africa: description of five new species (Nematoda: Dorylamidae). Nematologica, 3, 57–72.

    Article  Google Scholar 

  • Luc, M. (1981). Observations on some Xiphinema species with the female anterior genital branch reduced or absent (Nematoda: Longidoridae). Revue de Nématologie, 4, 157–167.

    Google Scholar 

  • Luc, M., & Coomans, A. (1992). Les nématodes phytoparasites du genre Xiphinema (Longidoridae) en Guyane et en Martinique. Belgian Journal of Zoology, 122, 147–183.

    Google Scholar 

  • Luc, M., & Doucet, M. E. (1990). La familia Longidoridae Thorne, 1935 (Nemata) en Argentina. 1. Distribución. Revista de Ciencias Agropecuarias, 7, 19–25.

    Google Scholar 

  • Luc, M., & Hunt, D. J. (1978). Redescription of Xiphinema longicaudatum Luc, 1961 and observations on X. krugi Lordello, 1955 (Nemata: Longidoridae). Nematologica, 24, 1–18.

    Article  Google Scholar 

  • Oliveira, C. M., Brown, D. J., Neilson, R., Monteiro, A. R., Ferraz, L. C. C., & Lamberti, F. (2003). The occurrence and geographic distribution of Xiphinema and Xiphidorus species (Nematoda: Longidoridae) in Brazil. Helminthologia, 40, 41–54.

    Google Scholar 

  • Oliveira, C. M. G., Ferraz, L. C. C. B., & Neilson, R. (2006). Xiphinema krugi, species complex or complex of cryptic species? Journal of Nematology, 38, 418–428.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Page, R. D. (1996). TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12, 357–358.

    CAS  PubMed  Google Scholar 

  • Peraza-Padilla, W., Archidona-Yuste, A., Cantalapiedra-Navarrete, C., Zamora-Araya, T., Palomares-Rius, J. E., & Castillo, P. (2016). Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda, Longidoridae) in Costa Rica. European Journal of Plant Pathology, 146, 000–000. doi:10.1007/s10658-016-0997-4.

    Google Scholar 

  • Razak, A. R., & Loof, P. A. A. (1999). The genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Western Malaysia. Fundamental and Applied Nematology, 21, 413–428.

    Google Scholar 

  • Robbins, R. T., & Brown, D. J. F. (1991). Comments on the taxonomy, occurrence and distribution of Longidoridae (Nematoda) in North America. Nematologica, 37, 395–419.

    Article  Google Scholar 

  • Seinhorst, J. W. (1966). Killing nematodes for taxonomic study with hot f.a. 4:1. Nematologica, 12, 178.

    Article  Google Scholar 

  • Siddiqi, M. R. (2000). Four new species of Xiphinema Cobb (Dorylaimida) lacking anterior ovary from South America. International. Journal of Nematology, 10, 234–243.

    Google Scholar 

  • Subbotin, S.A., Rogozhin, E., & Chizhov, V. (2014). Molecular characterisation and diagnostics of some Longidorus species (Nematoda: Longidoridae) from Russia and other countries using rRNA genes. European Journal of Plant Pathology, 138, 377–390.

  • Swart, A., & Quénéhervé, P. (1998). The genus Xiphinema (Nematoda: Longidoridae) in Guyane and Martinique. Fundamental and Applied Nematology, 21, 581–604.

    Google Scholar 

  • Volcy, C. (1990). Presencia de Xiphinema krugi en Antioquia/Colombia y observaciones sobre su variación morfológica. Fitopatologia Colombiana, 14, 18–23.

    Google Scholar 

  • Vrain, T. C., Wakarchuk, D. A., Levesque, A. C., & Hamilton, R. I. (1992). Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology, 15, 563–573.

    Google Scholar 

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Acknowledgements

Authors thank J. Martín Barbarroja and G. León Ropero from IAS-CSIC for the excellent technical assistance. Research was supported by grant of Universidad Nacional and FIDA (Fondo Institucional al Desarrollo Académico) from Costa Rica.

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Authors and Affiliations

  1. Laboratorio de Nematología, Escuela de Ciencias Agrarias, Universidad Nacional, Heredia, 86-3000, Costa Rica

    Walter Peraza-Padilla & Tatiana Zamora-Araya

  2. Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004, Córdoba, Spain

    Antonio Archidona-Yuste, Carolina Cantalapiedra-Navarrete, Juan E. Palomares-Rius & Pablo Castillo

  3. Department of Entomology and Nematology, University of California Davis, One Shields Avenue, Davis, CA, 95616-8751, USA

    Howard Ferris

  4. Plant Pest Diagnostic Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA, 95832, USA

    Sergei A. Subbotin

  5. Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Leninskii Prospect 33, Moscow, 117071, Russia

    Sergei A. Subbotin

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  1. Walter Peraza-Padilla
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  2. Antonio Archidona-Yuste
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  7. Sergei A. Subbotin
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  8. Pablo Castillo
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Correspondence to Pablo Castillo.

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Peraza-Padilla, W., Archidona-Yuste, A., Ferris, H. et al. Molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Costa Rica, with notes on Xiphinema setariae Tarjan, 1964. Eur J Plant Pathol 148, 739–747 (2017). https://doi.org/10.1007/s10658-016-1124-2

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