European Journal of Plant Pathology

, Volume 148, Issue 2, pp 367–377 | Cite as

Fusarium species associated with date palm in Saudi Arabia

  • Amgad A. Saleh
  • Anwar H. Sharafaddin
  • Mahmoud H. El_Komy
  • Yasser E. Ibrahim
  • Younis K. Hamad
  • Younis Y. Molan
Article
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Abstract

Fusarium is one of the most destructive fungal genera whose members cause many diseases on plants, animals, and humans. Moreover, many Fusarium species secrete mycotoxins (e.g. trichothecenes and fumonisins) that are toxic to humans and animals. Fusarium isolates from date palm trees showing disease symptoms, e.g. chlorosis, necrosis and whitening, were collected from seven regions across Saudi Arabia. After single-sporing, the fungal strains were morphologically characterized. To confirm the identity of morphologically characterized Fusarium strains, three nuclear loci, two partial genes of translation elongation factor 1 α (tef1α) and β-tubulin (tub2), and the rDNA-ITS region, were amplified and sequenced. Of the 70 Fusarium strains, 70 % were identified as F. proliferatum that were recovered from six regions across Saudi Arabia. Fusarium solani (13 %), as well as one strain each of the following species: F. brachygibbosum, F. oxysporum, and F. verticillioides were also recovered. In addition, five Fusarium-like strains were recognized as Sarocladium kiliense by DNA-based data. The preliminary in vitro pathogenicity results showed that F. proliferatum had the highest colonization abilities on date palm leaflets, followed by F. solani. Although F. oxysporum f. sp. albedinis is the most serious date palm pathogen, F. proliferatum and F. solani are becoming serious pathogens and efforts should be made to restrict and control them. In addition, the potential toxin risks of strains belonging to F. proliferatum should be evaluated.

Keywords

Date palm Fusarium proliferatum Fusarium solani DNA-based data 

Notes

Acknowledgment

This Project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number (11-AGR1475-02).

Supplementary material

10658_2016_1095_MOESM1_ESM.docx (34 kb)
ESM 1(DOCX 34 kb)
10658_2016_1095_MOESM2_ESM.docx (386 kb)
ESM 2(DOCX 386 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2016

Authors and Affiliations

  • Amgad A. Saleh
    • 1
    • 2
  • Anwar H. Sharafaddin
    • 1
  • Mahmoud H. El_Komy
    • 1
    • 3
  • Yasser E. Ibrahim
    • 1
    • 3
  • Younis K. Hamad
    • 1
    • 4
  • Younis Y. Molan
    • 1
  1. 1.Department of Plant Protection, College of Food and Agriculture SciencesKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Agriculture Research CenterAgricultural Genetic Engineering Research InstituteGizaEgypt
  3. 3.Agriculture Research CenterPlant Pathology InstituteGizaEgypt
  4. 4.Plant Pathology Department, Faculty of AgricultureAlexandria UniversityAlexandriaEgypt

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