Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia

Abstract

The occurrence and distribution of Dothistroma needle blight (DNB) on Pinus mugo was studied in 2014–2015 around the Slovakia. In total, 42 localities were investigated both native and planted ones. Symptoms of DNB were observed on 35 localities only on planted shrubs. All these 35 localities are new P. mugo DNB stands. No DNB symptoms were observed in natural and naturally regenerated plantations. DNA was extracted from a total of 236 isolates and eight needle samples. Based on the ITS-rDNA comparisons and using species specific primers, both pathogenic Dothistroma species were detected: D. septosporum and D. pini. Isolates of D. septosporum had ITS sequences identical to D. septosporum from Europe and both mating types were identified with slight predominance of MAT2. The ratio of D. septosporum mating types varies significantly between sites, ranging from an equal proportion of each mating type to single mating type populations. D. pini ITS sequence grouped with D. pini from Ukraine, Russia and Switzerland and only MAT2 was found.

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References

  1. Barnes, I., Crous, P. W., Wingfield, M. J., & Wingfield, B. D. (2004). Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini. Studies in Mycology, 50, 551–565.

    Google Scholar 

  2. Barnes, I., Kirisits, T., Akulov, A., Chhetri, D.B., Wingfield, M.J., Bulgakov, T.S, Wingfield, B.D. (2007). New reports of Dothistroma needle blight in Eurasian countries. Acta Silvatica and Lignaria Hungarica, Special Edition 2007, 237–238.

  3. Barnes, I., Kirisits, T., Akulov, A., Chhetri, D. B., Wingfield, B. D., Bulgakov, T. S., & Wingfield, M. J. (2008). New host and country records of the Dothistroma needle blight pathogens from Europe and Asia. Forest Pathology, 38(3), 178–195.

    Article  Google Scholar 

  4. Barnes, I., Kirisits, T., Wingfield, M. J., & Wingfield, B. D. (2011). Needle blight of pine caused by two species of Dothistroma in Hungary. Forest Pathology, 41(5), 361–369.

    Article  Google Scholar 

  5. Barnes, I., Wingfield, M. J., Carbone, I., Kirisits, T., & Wingfield, B. D. (2014). Population structure and diversity in an invasive pine needle pathogen reflects anthropogenic activity. Ecology and Evolution, 4(18), 3642–3661. doi:10.1002/ece3.1200.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Bednářová, M., Palovčíková, D., & Jankovský, L. (2006). The host spectrum of Dothistroma needle blight Mycosphaerella pini E. Rostrup – new hosts of Dothistroma needle blight observed in the Czech Republic. Journal of Forest Science, 52(1), 30–36.

    Google Scholar 

  7. Bergová, E., & Kryštofová, A. (2014). First report of Dothistroma pini, agent of the Dothistroma needle blight, on Pinus in the Czech Republic. Mykologické listy, 129, 18–19.

    Google Scholar 

  8. Boroń, P., Lenart-Boroń, A., & Mullett, M. (2016). The distribution of Dothistroma septosporum and its mating types in Poland. Forest Pathology, 46(5), 489–496. doi:10.1111/efp.12262.

  9. Bradshaw, R. E. (2004). Dothistroma (red-band) needle blight of pines and the dothistromin toxin. Forest Pathology, 34(3), 163–185.

    Article  Google Scholar 

  10. Butin, H. (1985). Teleomorph- und anamorph-Entwicklung von Scirrhia pini Funk & Parker auf Nadeln von Pinus nigra Arnold. [teleomorph and anamorph development of Scirrhia pini Funk & Parker of needles from Pinus nigra Arnold]. Sydowia, Annales Mycologici Ser. II, 38, 20–27.

    Google Scholar 

  11. Butin, V., & Richter, J. (1983). Dothistroma needle blight: a new disease on pine in the Federal Republic of Germany. Nachrichtenbl Deut Pflanzenschutzd, 35, 129–131.

    Google Scholar 

  12. Doroguine, M. (1911). Une maladie cryptogamique du Pin. Bulletin de la Société Mycologique de France, 27, 105–106.

    Google Scholar 

  13. Fabre, B., Ioos, R., Piou, D., & Marçais, B. (2012). Is the emergence of Dothistroma needle blight of pine in France caused by the cryptic species Dothistroma pini? Phytopathology, 102(1), 47–54.

    CAS  Article  PubMed  Google Scholar 

  14. Foffová, E., & Foff, V. (2007). Sypavky borovíc spôsobené fytokaranténnymi hubami z rodu Mycosphaerella na Slovensku [needle blight of pines caused by quarantine fungi from genus Mycosphaerella in Slovakia]. In M. Kodrík & P. Hlaváč (Eds.), Ochrana lesa 2007- Zborník vedeckých a odborných prác z medzinárodnej konferencie (pp. 59–64). Zvolen: Technická univerzita.

    Google Scholar 

  15. Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specifity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2), 113–118.

    CAS  Article  PubMed  Google Scholar 

  16. Gibson, I. A. S. (1972). Dothistroma blight of Pinus radiata. Annual Review of Phytopathology, 10, 51–72.

    Article  Google Scholar 

  17. Gibson, I. A. S., Christensen, P. S., & Munga, F. M. (1964). First observations in Kenya of a foliage disease of pines caused by Dothistroma pini Hulbary. Commonwealth Forestry Review, 43, 31–42.

    Google Scholar 

  18. Groenewald, M., Barnes, I., Bradshaw, R. E., Brown, A., Dale, A., Groenewald, J. Z., Lewis, K. J., Wingfield, B. D., Wingfield, M. J., & Crous, P. W. (2007). Characterization and worldwide distribution of the mating type genes in the Dothistroma needle blight pathogens. Phytopathology, 97(8), 825–834.

    CAS  Article  PubMed  Google Scholar 

  19. Hanso, M., & Drenkhan, R. (2008). First observations of Mycosphaerella pini in Estonia. Plant Pathology, 57(6), 1177.

    Article  Google Scholar 

  20. Ioos, R., Fabre, B., Saurat, C., Fourrier, C., Frey, P., & Marcais, B. (2010). Development, comparison, and validation of real-time and conventional PCR tools for the detection of the fungal pathogens causing brown spot and red band needle blights of pine. Phytopathology, 100(1), 105–114.

    CAS  Article  PubMed  Google Scholar 

  21. Jovaišiené, Z., & Pavilionis, R. (2005). Mycosphaerella pini is causing red band needle blight of pine in Lithuania (Pušinis rutulgrybis (Mycosphaerella pini) – raudunjuostés spyglių degligés sukéléjas Lietuvoje). Mūsų girios, 5, 7 (in Lithuanian).

    Google Scholar 

  22. Karadžič, D. (1989). Scirrhia pini Funk et Parker. Life cycle of the fungus in plantations of Pinus nigra Arn. in Serbia. European Journal of Forest Pathology, 19(4), 231–236.

    Article  Google Scholar 

  23. Karadžič, D. (2004). The distribution, hosts, epidemiology, impact and control of the fungus Mycosphaerella pini E. Rostrup apud and Munk. in Serbia. Bulletin of the Faculty of Forestry University in Belgrad, 90, 7–36.

    Google Scholar 

  24. Kehr, R., Pehl, L., Wulf, A., Schröder, T., & Kaminski, K. (2004). Zur Gefährdung von Bäumen und Waldökosystemen durch eingeschleppte Krankheiten und Schädlinge [introduced pests and diseases as risks to trees and forest ecosystems]. Nachrichtenbl Deut Pflanzenschutzd, 56, 217–238.

    Google Scholar 

  25. Kirisits, T., & Cech, L. T. (2006). Entwickelt sich die Dothistroma-Nadelbräune zu einem Forstschutzproblem in Österreich? [is Dothistroma needle blight going to become an important forest disease in Austria?]. Forstschutz Aktuell, 36, 20–26 http://www.waldwissen.net/themen/waldschutz/pilze_nematoden/bfw_nadelbraeune_kiefer_2006_DE). Accessed 22 February 2016.

    Google Scholar 

  26. Kowalski, T., & Jankowiak, R. (1998). First record of Dothistroma septospora (Dorog.) Morelet in Poland: a contribution to the symptomology and epidemiology. Phytopathologia Polonica, 16, 16–29.

    Google Scholar 

  27. Kriticos, D. J., Sutherst, R. W., Brown, J. R., Adkins, S. A., & Maywald, G. F. (2003a). Climate change and biotic invasions: a case history of a tropical woody vine. Biological Invasions, 5(3), 147–165.

    Article  Google Scholar 

  28. Kriticos, D. J., Sutherst, R. W., Brown, J. R., Adkins, S. A., & Maywald, G. F. (2003b). Climate change and the potential distribution of an invasive alien plant: Acacia nilotica ssp. indica in Australia. Journal of Applied Ecology, 40(1), 111–124.

    Article  Google Scholar 

  29. Kunca, A., Foffová, E. (2000). Ohrozenie porastov borovice čiernej fytokaranténnym patogénom Dothistroma septospora (Dorog.) Merelet [The threat to Austrian pine plantations from the phytosanitary pathogen Dothistroma septospora (Dorog) Morelet]. In J. Varínsky (Ed.), Aktuálne problémy v ochrane lesa 2000, (pp. 136–139), Zvolen: Lesnícky výskumný ústav (LVU). (in Slovak).

  30. Kunca, A., Brutovský, D., Finďo, S., Galko, J., Gubka, A., Kaštier, P., Konôpka, B., Leontovyč, R., Longauerová, V., Nikolov, C., Vakula, J., Varínsky, J., & Zúbrik, M. (2010). Problémy ochrany lesa v roku 2009 a prognóza na rok 2010 [problems in forest protectionin the 2009 and prognosis for the year 2010]. In A. Kunca (Ed.), Aktuálne problémy v ochrane lesa 2010 (pp. 5–18). Zvolen: Národné lesnícke centrum (in Slovak).

    Google Scholar 

  31. Maschning, E., & Pehl, L. (1994). Bedrohung autochthoner Latschen durch Dothistroma-Nadelbräune [threat to authochthonous dwarf mountain pine by Dothistroma needle blight]. Allgemeine Forstzeitschrift, 49, 249–252.

    Google Scholar 

  32. Pastirčáková, K., Ivanová, H., Pastirčák, M. (2014). Druhová diverzita húb na boroviciach (Pinus spp.) v mestskej a mimomestskej vegetácii [Fungal species diversity in pines (Pinus spp.) in urban nonurban greenery]. In M. Barta, P. Ferus (Eds.) Dendrologické dni v Arboréte Mlyňany SAV 2014 recenzovaný zborník príspevkov z vedeckej konferencie (pp. 150–157). Vieska nad Žitavou: Arborétum Mlyňany SAV. (in Slovak).

  33. Pehl, L., & Butin, H. (1992). Dothistroma septospora, a new fungus pest on Pinus mugo. Allgemeine Forst Zeitschrift der Wald, 47, 758–760.

    Google Scholar 

  34. Piou, D., & Ioos, R. (2014). First report of Dothistroma pini, a recent agent of the Dothistroma needle blight, on Pinus radiata in France. Plant Diseases, 98(6), 841. doi:10.1094/PDIS-01-13-0068-PDN.

    Article  Google Scholar 

  35. Piskur, B., Hauptman, T., & Jurc, D. (2013). Dothistroma needle blight in Slovenia is caused by two cryptic species: Dothistroma pini and Dothistroma septosporum. Forest Pathology, 46(6), 518–521.

    Article  Google Scholar 

  36. Queloz, V., Wey, T., & Holdenrieder, O. (2014). First record of Dothistroma pini on Pinus nigra in Switzerland. Plant Disease, 98(12), 1744.2–1744.2.

    Article  Google Scholar 

  37. Schmitz, S., Gischer, F., Chandelier, A. (2013). First detection of Dothistroma pini in Belgium. In Book of Abstracts of the Conference of IUFRO Working Party 7.02.02 Foliage, shoot and stem diseases of forest trees, ‘Biosecurity in natural forests and plantations, genomics and biotechnology for biosecurity in forestry’. Brno: Mendel University.

  38. Tomšovský, M., Tomešová, V., Palovčíková, D., Kostovčík, M., Rohrer, M., Hanáček, P., & Jankovský, L. (2013). The gene flow and mode of reproduction of Dothistroma septosporum in the Czech Republic. Plant Pathology, 62(1), 59–68.

    Article  Google Scholar 

  39. van der Pas, J. B. (1981). Reduced early growth rates of Pinus radiata caused by Dothistroma pini. New Zealand Journal of Forestry Science, 11, 210–220.

    Google Scholar 

  40. Watt, M. S., Kriticos, D. J., Alcaraz, S., Brown, A. V., & Leriche, A. (2009). The hosts and potential geographic range of Dothistroma needle blight. Forest Ecology and Management, 257(6), 1505–1519.

    Article  Google Scholar 

  41. White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: a sequencing guide to methods and applications (pp. 315–322). San Diego: Academic Press.

    Google Scholar 

  42. Woods, A. J. (2003). Species diversity and forest health in Northwest British Columbia. The Forestry Chronicle, 79(5), 892–897.

    Article  Google Scholar 

  43. Zúbrik, M., Kunca, A., Turčani, M., Vakula, J., & Leontovyč, R. (2006). Invasive and quarantine pests in forests in Slovakia. Bulletin OEPP/EPPO Bulletin, 36(2), 402–408.

    Article  Google Scholar 

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Acknowledgments

This study was financed by the Slovak VEGA grant agency, project 2/0069/14 and is a contribution to the project Diarod funded by EU COST Action (FP1102).

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Correspondence to K. Adamčíková.

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Ondrušková, E., Hečková, Z., Horáková, M.K. et al. Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia. Eur J Plant Pathol 148, 283–294 (2017). https://doi.org/10.1007/s10658-016-1088-2

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Keywords

  • Dothistroma septosporum
  • D. pini
  • Pinus mugo
  • Slovakia
  • Mating types