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Differential expression of genes during the interaction between Colletotrichum lindemuthianum and Phaseolus vulgaris

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Abstract

The fungus Colletotrichum lindemuthianum is the causal agent of anthracnose, one of the most severe diseases of the common bean (Phaseolus vulgaris). The infection process begins with the adhesion of conidia to the plant’s surface. Appressoria are then formed, allowing penetration of the fungus. Next, the biotrophic phase begins, followed by the necrotrophic phase. Due to the peculiar nutrition mode of the fungus, including both of the previously mentioned stages, it is of great interest to determine which genes are involved in the transition between the two phases during the infection process. To determine this, suppression subtractive hybridization (SSH) was used in association with qRT-PCR in the present study. These methods allowed for the identification of genes that were differentially expressed at each developmental stage of the fungus in the plant. This is the first report on the use of the cited techniques to evaluate the infectious cycle of the fungus. A total of 175 sequences exhibited significant identity (e ≤ 10−5) with sequences present in the sequenced genomes of P. vulgaris and C. lindemuthianum; approximately 41 % of those were determined to belong to the fungus, and 59 % were determined to belong to the plant. Of the predicted sequences, 68 % were of unknown function or were not found in the databases. Among the analyzed expressed sequence tags (ESTs), sequences were found that encode proteins related to: primary and secondary metabolism; the transport of different compounds; the degradation/modification of proteins; cell regulation and signaling; cellular stress response; and the degradation of exogenous compounds. The obtained results allowed for the identification of sequences encoding proteins that are essential for the progression of anthracnose. Furthermore, it was possible to identify new genes, the functions of which have not yet been described, and even to identify unique genes of C. lindemuthianum that are involved in the pathogenicity and virulence of this fungus.

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Acknowledgments

This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). We thank Professor Everaldo Gonçalves de Barros for providing the isolate A2 2-3 of race 89 C. lindemuthianum.

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  1. Embrapa Hortaliças, CEP 70351-970, Brasília, Distrito Federal, Brazil

    Mariana R. Fontenelle

  2. Laboratório de Genética Molecular e de Microrganismos, BIOAGRO, Departamento de Microbiologia, Universidade Federal de Viçosa, CEP 36570-000, Viçosa, Minas Gerais, Brazil

    Mateus F. Santana, Andréia Cnossen, Denise M. S. Bazzolli, Elza F. de Araújo & Marisa V. de Queiroz

  3. Laboratório de Genômica, BIOAGRO, Departamento de Fitopatologia, Universidade Federal de Viçosa, CEP 36570-000, Viçosa, Minas Gerais, Brazil

    Sérgio H. Bromonschenkel

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Fontenelle, M.R., Santana, M.F., Cnossen, A. et al. Differential expression of genes during the interaction between Colletotrichum lindemuthianum and Phaseolus vulgaris . Eur J Plant Pathol 147, 653–670 (2017). https://doi.org/10.1007/s10658-016-1033-4

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