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Virulence spectrum of populations of Pyricularia oryzae in irrigated rice ecosystems in Kenya

Abstract

Pyricularia oryzae is a common pathogen in rice fields causing significant yield loss in Kenya. The virulence spectrum of the populations of P. oryzae in irrigated ecosystems in Kenya is not fully understood. Fifty-one rice lines carrying single resistance genes, recurrent parents and 3 local varieties were field-evaluated at Mwea, West Kano and Gamba to characterize virulence spectrum of populations of P. oryzae. West Kano, Mwea and Gamba had 68.63 %, 49.02 % and 40.2 %, respectively of the lines showing susceptibility to the populations of P. oryzae. The virulence spectrum of the populations of P. oryzae varied significantly suggesting the existence of a high pathogenic variability at the three locations. Lines with Piz-t, Pik-s, Pik-p, Pik-h, Piz-5, Piz, Pit, Pish, Pi1, Pi5 (t), Pi12 (t), Pik-m, Pita-2, Pib and Pik were resistant to the population of P. oryzae at various locations. A local variety, BW196 was resistant to the populations of P. oryzae across the locations. Some lines carrying the same resistance genes showed different reaction to the populations of P. oryzae, suggesting existence of additional genes. Variation in resistance reaction was observed for some rice lines carrying the same genes in the CO39 and Lijiangxintuanheigu (LTH) genetic backgrounds. Most promising resistance genes observed in this study would be introgressed into preferred yet susceptible rice varieties in Kenya. Resistance gene(s) in BW196 should be identified for inclusion in breeding programs.

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Abbreviations

MOA:

Ministry of Agriculture

GAIN:

Global Agricultural Information Network

IEA:

Institute of Economic Affairs

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Acknowledgments

This work was supported by the International Rice Research Institute through Japan Breeding Project under Budget code No.PC034J100000240. The authors thank Dr. Casiana M. Vera Cruz, Dr. Hei Leung and Dr. Kenneth McNally for providing a set of differential rice lines. Mwea Irrigation Agricultural Development, West Kano National Irrigation Board and Tana and Athi Rivers Development Authority are acknowledged for providing irrigation facilities and research fields. We are grateful to Dr. John M. Kimani, Ms. Winnie Kore, Mr. Jeremy J. Njoka and Mr. Benjamin Mwakio for assistance during planning and implementing the field activities.

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Correspondence to Beatrice A. Were.

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Nyongesa, B.O., Bigirimana, J., Were, B.A. et al. Virulence spectrum of populations of Pyricularia oryzae in irrigated rice ecosystems in Kenya. Eur J Plant Pathol 146, 911–922 (2016). https://doi.org/10.1007/s10658-016-0969-8

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Keywords

  • Blast variability
  • Differential rice lines
  • Natural infection
  • Resistance genes