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Incidence and etiology of postharvest fungal diseases of loquat fruit (Eriobotrya japonica (Thunb.) Lindl. cv. ‘Algerie’) in Alacant province (Spain)

European Journal of Plant Pathology volume 146, pages 847–860 (2016)Cite this article

Abstract

‘Algerie’ is currently the most important loquat cultivar in Spain. The incidence and etiology of postharvest diseases affecting this cultivar were determined under local conditions. Latent and wound pathogens were evaluated for two consecutive seasons on commercially grown loquats from two orchards. Healthy loquats were either surface-disinfected or artificially wounded in the rind and placed in humid chambers at 20 °C for up to 5 weeks. Additionally, decay was assessed on commercially-handled loquats stored at 5 °C for up to 20 weeks. The most frequent disease was caused by Alternaria alternata, followed by Penicillium expansum. These two pathogens were present on fruit assessed for all types of infection. Moreover, decay caused by Botrytis cinerea was abundantly observed on both wounded and cold-stored fruit, while Colletotrichum gloeosporioides was frequently found on surface-disinfected fruit. Other pathogens that were observed causing latent infection to a lesser extent included Pestalotiopsis clavispora and Diplodia seriata. Common isolates were identified by macroscopic and microscopic morphology and/or DNA amplification and sequencing. Pathogenicity of selected isolates was demonstrated by fulfilling Koch’s postulates and disease development was assessed on artificially inoculated loquats stored at either 20 or 5 °C.

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References

  • Batta, Y. (2005). Control of Alternaria spot disease on loquat (Eriobotrya japonica Lindl.) using detached fruits and leaf-disk assay. An-Najah University Journal for Research - Natural Sciences, 19, 69–82.

    CAS  Google Scholar 

  • Butt, H., Mukhtar, T., Batool, M., Aslam, M. N., Kayani, M. Z., & Hussain, M. A. (2015). Occurrence of leaf scab caused by Spilocaea pyracanthae on loquat (Eriobotrya japonica) in the Pothowar region of Pakistan. Mycopathologia, 13, 29–32.

    Google Scholar 

  • Cao, S. F., & Zheng, Y. H. (2010). Effect of 1-methylcyclopropene on anthracnose rot caused by Colletotrichum acutatum and disease resistance in loquat fruit. Journal of the Science of Food and Agriculture, 90, 2289–2294.

    CAS  Article  PubMed  Google Scholar 

  • Cao, S. F., Cai, Y., Yang, Z. F., Joyce, C. D., & Zheng, Y. (2014). Effect of MeJA treatment on polyamine, energy status and anthracnose rot of loquat fruit. Food Chemistry, 145, 86–89.

    CAS  Article  PubMed  Google Scholar 

  • Cao, S. F., Zheng, Y. H., Yang, Z. F., Tang, S. S., Jin, P., Wang, K. T., et al. (2008). Effect of methyl jasmonate on the inhibition of Colletotrichum acutatum infection in loquat fruit and the possible mechanisms. Postharvest Biology and Technology, 49, 301–307.

    CAS  Article  Google Scholar 

  • Cai, C., Xu, C., Shan, L., Li, X., Zhou, C., Zhang, W., et al. (2006). Low temperature conditioning reduces postharvest chilling injury in loquat fruit. Postharvest Biology and Technology, 41, 252–259.

    CAS  Article  Google Scholar 

  • Deng, C. J., Jiang, J. M., Zhang, X. Y., Xu, Q. Z., Zhang, X. J., & Wei, X. Q. (2012). Research on influence factors in heat injury of loquat peel. Fujian Journal of Agricultural Sciences, 27, 1081–1086.

    Google Scholar 

  • Ding, C. K., Chachin, K., Hamauzu, Y., Ueda, Y., & Imahori, Y. (1998). Effects of storage temperatures on physiology and quality of loquat fruit. Postharvest Biology and Technology, 14, 309–315.

    CAS  Article  Google Scholar 

  • Ding, C. K., Chachin, K., Ueda, Y., Imahori, Y., & Wang, C. Y. (2002). Modified atmosphere packaging maintains postharvest quality of loquat fruit. Postharvest Biology and Technology, 24, 341–348.

    CAS  Article  Google Scholar 

  • Ding, Z., Tian, S., Wang, Y., Li, B., Chan, Z., Han, L., et al. (2006). Physiological response of loquat fruit to different storage conditions and its storability. Postharvest Biology and Technology, 41, 143–150.

    CAS  Article  Google Scholar 

  • Droby, S., Wisniewski, M., & Benkeblia, N. (2011). Postharvest pathology of tropical and subtropical fruit and strategies for decay control. In E. M. Yahia (Ed.), Postharvest biology and technology of tropical and subtropical fruits (Vol. 1, pp. 194–223). Cambridge, UK: Woodhead Publishing Limited.

    Chapter  Google Scholar 

  • Ghasemnezhad, M., Nezhad, M. A., & Gerailoo, G. (2011). Changes in postharvest quality of loquat (Eriobotrya japonica) fruits influenced by chitosan. Horticulture, Environment, and Biotechnology, 52, 40–45.

    CAS  Article  Google Scholar 

  • Gu, H., Liu, A. Y., Chen, W. X., Feng, S. J., & Shi, J. Y. (2007). Development and control of postharvest diseases of loquat fruit. Acta Horticulturae, 750, 437–444.

    Article  Google Scholar 

  • Jones, A. L., & Aldwinckle, H. S. (1990). Compendium of apple and pear diseases. St. Paul, MN, USA: The American Phytopathological Society Press.

  • Ko, Y., Liu, C. W., Chen, C. C., Yao, K. S., Maruthasalam, S., & Lin, C. H. (2010). First report of fruit rot of loquat caused by an Alternaria sp. in Taiwan. Plant Disease, 94, 481.

    Article  Google Scholar 

  • Kobiler, I., Akerman, M., Huberman, L., & Prusky, D. (2011). Integration of pre- and postharvest treatments for the control of black spot caused by Alternaria alternata in stored persimmon fruit. Postharvest Biology and Technology, 59, 166–171.

    CAS  Article  Google Scholar 

  • Liu, A. Y., Chen, W. X., Gu, H., Shi, J. Y., & Li, J. (2007). Biological characteristic of pathogenic fungus causing anthracnose of loquat fruit. Acta Horticulturae, 750, 465–447.

    CAS  Article  Google Scholar 

  • Liu, F., Tu, K., Shao, X., Zhao, Y., Tu, S., Su, J., et al. (2010). Effect of hot air treatment in combination with Pichia guilliermondii on postharvest anthracnose rot of loquat fruit. Postharvest Biology and Technology, 58, 65–71.

    CAS  Article  Google Scholar 

  • Lu, Z. M., Zhang, Z. L., Wu, W. X., & Li, W. H. (2007). Effect of low temperatures on postharvest loquat fruit. Acta Horticulturae, 750, 483–486.

    Article  Google Scholar 

  • MAGRAMA (2015). Anuario de Estadística 2014. Resource document Ministerio de Agricultura, Alimentación y Medio Ambiente (in Spanish). http://www.magrama.gob.es/estadistica/pags/anuario/2014/AE_2014_Completo.pdf. Accessed 12 January 2016.

  • Mirhosseini, H. A., Babaeizad, V., & Basavand, E. (2015). Identification and detection of agent of loquat leaf spot and fruit rot in north of Iran. Journal on New Biological Reports, 4, 135–138.

    Google Scholar 

  • Molinu, M. G., D’Hallewin, G., Dore, A., Serusi, A., Venditti, T., & Agabbio, M. (2005). Carbonic acid salts at 25 or 45 °C to control loquat decay under shelf life conditions. Communications in Agricultural and Applied Biological Sciences, 70, 365–370.

    CAS  PubMed  Google Scholar 

  • Palou, L., Montesinos-Herrero, C., Tarazona, I., Besada, C., & Taberner, V. (2015). Incidence and etiology of postharvest fungal diseases of persimmon (Diospyros kaki Thunb. cv. Rojo Brillante) in Spain. Plant Disease, 99, 1416–1425.

    CAS  Article  Google Scholar 

  • Palou, L., Montesinos-Herreros, C., Guardado, A., & Taberner, V. (2013a). First report of Pestalotiopsis clavispora causing postharvest fruit rot of loquat in Spain. Journal of Plant Pathology, 95, S4.69.

    Google Scholar 

  • Palou, L., Taberner, V., & Montesinos-Herreros, C. (2013b). First report of Diplodia seriata causing loquat fruit rot in Spain. Plant Disease, 97, 421.

    Article  Google Scholar 

  • Palou, L., Taberner, V., Guardado, A., del Río, M. A., & Montesinos-Herreros, C. (2013c). Incidence and etiology of postharvest fungal diseases of pomegranate (Punica granatum cv. Mollar de Elche) in Spain. Phytopathologia Mediterranea, 52, 478–489.

    Google Scholar 

  • Pareek, S., Benkeblia, N., Janick, J., Cao, S., & Yahia, E. M. (2014). Postharvest physiology and technology of loquat (Eriobotrya japonica Lindl.) fruit. Journal of the Science of Food and Agriculture, 94, 1495–1504.

    CAS  Article  PubMed  Google Scholar 

  • Ploetz, R. C. (2003). Diseases of tropical fruit crops. Wallingford, UK: CABI Publishing.

    Book  Google Scholar 

  • Rotem, J. (1998). The genus Alternaria. Biology, epidemiology and pathogenicity (2nd ed.). St. Paul, MN, USA: The American Phytopathological Society Press.

    Google Scholar 

  • Sánchez-Torres, P., Hinarejos, R., & Tuset, J. J. (2007). Identification and characterization of Fusicladium eriobotryae: fungal pathogen causing Mediterranean loquat scab. Acta Horticulturae, 750, 343–347.

    Article  Google Scholar 

  • Sánchez-Torres, P., Hinarejos, R., & Tuset, J. J. (2009). Characterization and pathogenicity of Fusicladium eriobotryae, the fungal pathogen responsible for loquat scab. Plant Disease, 93, 1151–1157.

    Article  Google Scholar 

  • Soler, E., Martínez-Calvo, J., Llácer, G., & Badenes, M. L. (2007). Loquat in Spain: production and marketing. Acta Horticulturae, 750, 45–47.

    Article  Google Scholar 

  • Sultan, M. Z., Khalefa, S. M., Elhamamsy, S. M., & Mostafa, Y. S. (2015). Effect of postharvest anti-oxidant treatments on loquat fruit deterioration during storage at room temperature. Acta Horticulturae, 1092, 173–179.

    Article  Google Scholar 

  • Sun, H., Zhang, L. L., Zhang, J. Z., & Hu, D. W. (2009). Identificacion of causal agent of blosson blight of loquat in Zhejiang province, China. Journal of Zhejiang University - Agricultural and Life Sciences, 35, 237–242.

    CAS  Google Scholar 

  • Tian, S., Li, B., & Ding, Z. (2007). Physiological properties and storage technologies of loquat fruit. Fresh Produce, 1, 76–81.

    Google Scholar 

  • Tian, S., Quin, G., & Li, B. (2011). Loquat (Eriobotrya japonica L). In E. M. Yahia (Ed.), Postharvest biology and technology of tropical and subtropical fruits (Vol. 3, pp. pp. 424–442). Cambridge, UK: Woodhead Publishing Limited.

  • Tziros, G. T. (2013). Alternaria alternata causes leaf spot and fruit rot on loquat (Eriobotrya japonica) in Greece. Australasian Plant Disease Notes, 8, 123–124.

  • Wang, K., Cao, S., Di, Y., Liao, Y., & Zheng, Y. (2015). Effect of ethanol treatment on disease resistance against anthracnose rot in postharvest loquat fruit. Scientia Horticulturae, 188, 115–121.

    CAS  Article  Google Scholar 

  • White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: a guide to methods and applications (pp. 315–322). San Diego, CA, USA: Academic Press Inc..

    Google Scholar 

  • Williamson, B., Tudzynski, B., Tudzynski, P., & van Kan, J. A. L. (2007). Botrytis cinerea the cause of grey mould disease. Molecular Plant Pathology, 8, 561–580.

    CAS  Article  PubMed  Google Scholar 

  • Yan, R., Ding, D., Guan, W., Hou, J., & Li, M. (2008). Control of grey mould rot of loquat with chitinase expressed in Pichia pastoris. Crop Protection, 27, 1312–1317.

    CAS  Article  Google Scholar 

Download references

Acknowledgments

This research was partially funded by Spanish ‘Ministerio de Ciencia e Innovación’ (MICINN; project AGL 2004-05271/AGR) and the European Union (FEDER Program). The authors thank Mr. Esteve Soler and the Cooperativa Agrícola de Callosa d’en Sarrià S. Coop. V. for providing fruit. In memory of Dr. Miguel Angel del Río, for his guidance and friendship.

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  1. Laboratori de Patologia, Centre de Tecnologia Postcollita (CTP), Institut Valencià d’Investigacions Agràries (IVIA), Apartat Oficial, 46113 Montcada, València, Spain

    Lluís Palou, Paloma Sánchez-Torres, Clara Montesinos-Herrero & Verònica Taberner

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  1. Lluís Palou
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  2. Paloma Sánchez-Torres
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  3. Clara Montesinos-Herrero
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  4. Verònica Taberner
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Correspondence to Lluís Palou.

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Palou, L., Sánchez-Torres, P., Montesinos-Herrero, C. et al. Incidence and etiology of postharvest fungal diseases of loquat fruit (Eriobotrya japonica (Thunb.) Lindl. cv. ‘Algerie’) in Alacant province (Spain). Eur J Plant Pathol 146, 847–860 (2016). https://doi.org/10.1007/s10658-016-0964-0

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Keywords

  • Eriobotrya japonica
  • Postharvest diseases
  • Latent infection
  • Wound infection
  • Cold storage
  • Pathogenicity