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Molecular analysis of Cercospora beticola isolates for strobilurin resistance from the Central High Plains, USA

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An Erratum to this article was published on 30 July 2016

Abstract

Cercospora leaf spot (CLS), caused by Cercospora beticola, is the most destructive foliar disease and is a problem in sugar beet production areas, such as Central High Plains (states of Colorado, Montana, Nebraska and Wyoming) in the United States. The disease can be controlled by strobilurin fungicides, referred to as quinone outside inhibitors (QoIs), with a single target site on C. beticola. Strobilurin resistance has been reported in beet production areas from the United States, including the Central High Plains. Although strobilurin resistance is quantitatively inherited, it is considered that it has low to medium heritability in the population. Effective diagnostic tools are required for the rapid detection of C. beticola strobilurin resistance. The study obtained a partial nucleotide sequence of the C. beticola cytochrome b gene and determined to a putative protein with ~386 amino acid residues. Eighty C. beticola isolates (2004–2011) from the Central High Plains were analyzed for mutations. We found a single nucleotide polymorphic (SNP) site which led to G143A mutation and was present in 2 C. beticola QoI-resistant isolates. Partial sequences obtained from 82 C. beticola QoI-sensitive isolates showed identical cytochrome b gene. We developed a PCR-RFLP assay that involved an in vitro digestion using Fnu4HI restriction enzyme for the rapid molecular detection of G143A mutation in the C. beticola population. Results indicated the PCR-RFLP assay was reliable, sensitive, and can be used for the rapid detection of C. beticola strobilurin resistance.

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Acknowledgments

This study was supported by the University of Wyoming Agricultural Experiment Station and Western Sugar Research Committee. Mention of trade names or commercial products in this article was solely for providing information and was neither a recommendation nor an endorsement of specific organizations mentioned.

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  1. Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, 6505 Mahan Drive, Tallahassee, FL, 32317, USA

    J. O. Obuya

  2. Plant Sciences Department-3354, College of Agriculture and Natural Sciences, University of Wyoming, 1000 E. University Ave, Laramie, WY, 82071, USA

    G. D. Franc

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G. D. Franc, is deceased.

An erratum to this article is available at http://dx.doi.org/10.1007/s10658-016-1015-6.

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Figure S1

Alignment of the partial cytochrome b gene sequences from nine C. beticola isolates using ClustalW2 Seven QoI-sensitive isolates had identical cytochrome b gene sequences, whereas two QoI-resistant isolates (UW11–112 and UW11–117) had a single point mutation at codon 143 as shown in red (JPEG 374 kb)

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Obuya, J.O., Franc, G.D. Molecular analysis of Cercospora beticola isolates for strobilurin resistance from the Central High Plains, USA. Eur J Plant Pathol 146, 817–827 (2016). https://doi.org/10.1007/s10658-016-0959-x

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