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European Journal of Plant Pathology

, Volume 145, Issue 2, pp 331–343 | Cite as

Molecular characterization of lipoxygenase genes and their expression analysis against biotic and abiotic stresses in Panax ginseng

  • Kwi-Sik Bae
  • Shadi RahimiEmail author
  • Yu-Jin Kim
  • Balusamy Sri Renuka Devi
  • Altanzul Khorolragchaa
  • Johan Sukweenadhi
  • Jeniffer Silva
  • Davaajargal Myagmarjav
  • Deok-Chun YangEmail author
Article

Abstract

Lipoxygenase (LOX) belongs to a family of non-heme-iron-containing fatty acid dioxygenases that are widely distributed in plants and animals. LOX involved in the synthesis of jasmonic acid and six-carbon (C6) volatiles which is necessary for plant growth and responses to a wide range of biotic and abiotic stresses. We have isolated and characterized LOX cDNA clones from Panax ginseng Meyer. From their deduced amino acid sequences, two diverse classes of 9-LOX (LOX1, LOX2, and LOX3) and 13-LOX (LOX4, LOX5) are defined in P. ginseng. A GenBank Blast X search revealed that the deduced amino acid of PgLOXs share a high degree of homology with LOX from other plants and mammals especially in three distinct motifs; motif1 harboring iron binding regions, motif2 and motif3. Chloroplast localization was predicted for PgLOX5. PgLOXs displayed organ-specific expression, highly expressed in aerial parts of the plant such as 3-year old flower, stem and leaf tissues. PgLOXs mRNAs were elevated strongly by bacterial infection. Expression of PgLOXs was differentially induced in ginseng not only by mechanical damage and methyl jasmonate but also after exposure to withholding water. Ginseng 13-LOXs positively respond to wounding that may involve in production of C6 volatiles and jasmonic acid at the wounded sites. However, the higher expression of PgLOX3 by water deficit and 82 % of the nucleotide sequence identity with the EST from severe drought-stressed leaves of Populus (CU229089.1) at +6371 bp downstream of PgLOX3 genomic DNA structure can suggest drought tolerance role for PgLOX3. Ginseng LOX genes have different expression pattern which may suggest different specific function against various environmental stresses.

Keywords

Abiotic stress Biotic stress Gene expression Jasmonic acid Lipoxygenase Panax ginseng 

Abbreviations

AOC

allene oxide cyclase

AOS

allene oxide synthase

EST

expressed sequence tag

HPL

hydroperoxide lyase

13-HPOD

(13S)-hydroperoxyoctadecadienoic acid

9-HPOD

(9S)-hydroperoxyoctadecadienoic acid

IBA

indole-3-butyric acid

JA

jasmonic acid

LOX

lipoxygenase

MJ

methyl jasmonate

OPDA

(9S,13S)-12-oxo phytodienoic acid

Pst

Pseudomonas syringae pv tomato strain DC3000

PUFAs

polyunsaturated fatty acids

RT-PCR

reverse transcriptase-polymerase chain reaction.

Notes

Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) by the Ministry of Education (2013R1A1A2064430), Republic of Korea (YJ Kim) and iPET (312064-03-1-HD040), Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, Republic of Korea (DC Yang).

Compliance with ethical standards

Conflict of interest

None.

Supplementary material

10658_2015_847_MOESM1_ESM.pdf (119 kb)
Online Resource 1 (PDF 118 kb)
10658_2015_847_MOESM2_ESM.pdf (90 kb)
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10658_2015_847_MOESM3_ESM.pdf (132 kb)
Online Resource 3 (PDF 131 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2016

Authors and Affiliations

  • Kwi-Sik Bae
    • 2
  • Shadi Rahimi
    • 2
    Email author
  • Yu-Jin Kim
    • 2
  • Balusamy Sri Renuka Devi
    • 2
  • Altanzul Khorolragchaa
    • 2
  • Johan Sukweenadhi
    • 2
  • Jeniffer Silva
    • 2
  • Davaajargal Myagmarjav
    • 2
  • Deok-Chun Yang
    • 1
    • 2
    Email author
  1. 1.Department of Oriental Medicinal BiotechnologyCollege of Life Sciences, Kyung Hee UniversityYonginRepublic of Korea
  2. 2.Graduate School of Biotechnology and Ginseng BankCollege of Life Sciences, Kyung Hee UniversityYonginRepublic of Korea

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