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European Journal of Plant Pathology

, Volume 145, Issue 1, pp 221–225 | Cite as

Vmp1 and stamp genes variability of ‘Candidatus phytoplasma solani’ in Bosnian and Herzegovinian grapevine

  • Duška DelićEmail author
  • Bachir BalechEmail author
  • Mariana Radulović
  • Biljana Lolić
  • Ana Karačić
  • Vera Vukosavljević
  • Gordana Đurić
  • Tatjana Jovanović Cvetković
Article

Abstract

Bois noir is one of the most important diseases caused by ‘Candidatus Phytoplasma solani’ affecting grapevine in the Euro-Mediterranean area. ‘Ca. P. solani’ characterization plays a significant role in its diagnosis, control and understanding of its epidemiology. In the present study, two genes encoding membrane proteins, namely vmp1 and stamp, were used to assess the genetic variability of 18 ‘Ca. P. solani’ grapevine isolates from the Herzegovina region (B&H), previously characterised as tuf-b type. Nested-PCR/RFLP and sequence analyses of vmp1 gene showed the presence of three profiles V17, V14 and V4 where V17 was highly prevalent. Phylogenetic analysis of stamp gene revealed the presence of four different genotypes, where Rqg50 was the most frequent. Finally, the combined results of tuf, vmp1 and stamp genes, reported three previously identified genotypes from Serbia, Austria and Montenegro, and two newly described ones (DB1 and DB2). Although performed only on ‘Ca. P. solani’ isolates from infected grapevines, this study provides useful molecular information to get better insight on BN epidemiology in B&H.

Keywords

Bois noir disease Genetic variability Membrane protein Nested PCR RFLP Sequencing Phylogeny 

Abbreviations

B&H

Bosnia and Herzegovina

BN

Bois noir

PCR

Polymerase chain reaction

RFLP

Restriction fragment length polymorphisms

Notes

Acknowledgments

This work was supported by the B&H Ministry of Civil Affairs (project number 10-35-5-673/13-16). We are very thankful to Xavier Foissac for helpful suggestion in determination of vmp1 restriction types. Special thanks also the anonymous reviewers for the helpful comments with which we clarified and upgraded this paper.

Supplementary material

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2015

Authors and Affiliations

  • Duška Delić
    • 1
    Email author
  • Bachir Balech
    • 2
    Email author
  • Mariana Radulović
    • 1
  • Biljana Lolić
    • 1
  • Ana Karačić
    • 3
  • Vera Vukosavljević
    • 4
  • Gordana Đurić
    • 1
  • Tatjana Jovanović Cvetković
    • 1
  1. 1.Faculty of AgricultureUniversity of BanjalukaBanjalukaBosnia and Herzegovina
  2. 2.Institute of Biomembranes and Bioenergetics (CNR)BariItaly
  3. 3.Federal Agromediterranean Institute of MostarMostarBosnia and Herzegovina
  4. 4.Faculty of AgronomyDepartment of Fruit Growing & ViticultureČačakSerbia

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