Skip to main content
SpringerLink
Log in

Diverse phytoplasmas associated with potato stolbur and other related potato diseases in Russia

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

A large scale survey on diseased potato plants that exhibited symptoms similar to those of stolbur, potato purple top wilt, potato witches’-broom, and potato round-leaf in eight Economical Regions of Russia was conducted from 2006 to 2012. A total of 1228 potatoes were collected and the associated phytoplasmas were identified. Phytoplasmas belonging to five 16S rRNA (16Sr) phytoplasma groups (16SrI, 16SrII, 16SrIII, 16SrVI, and 16SrXII) and at least eight subgroups (16SrI-B, 16SrI-C, 16SrI-P, 16SrII-A, 16SrIII-B, 16SrVI-A, 16SrVI-C, and 16SrXII-A) were identified. To our knowledge, subgroup 16SrIII-B and 16SrVI-C phytoplasmas have not been reported to infect potatoes. Based on the results of the survey, the percentages of infection caused by various phytoplasmas were: 40.2 % (16SrXII), 25.5 % (16SrIII), 20.9 % (16SrI), 12.4 % (16SrVI), and 1.2 % (16SrII). The results also indicated potato diseases exhibiting stolbur or similar symptoms could be caused by stolbur phytoplasma (16SrXII-A) alone or by other phytoplasma groups (e.g., 16SrI and 16SrIII). A survey on potential insect vectors was conducted from 2009 to 2012 in the Moscow oblast of the Central Region. A total of 23 different species of Hemiptera insects were screened for the presence of phytoplasmas. Eight leafhopper and three froghopper species carried phytoplasmas. Euscelis incisus and Macrosteles laevis were the most prevalent. The other species were present in very low populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Balakishiyeva, G., Danet, J. L., Gurbanov, M., Mamedov, A., Kheyr-Pour, A., & Fossac, X. (2010). First report of phytoplasma infections in several temperate fruit trees and vegetable crops in Azerbaijan. Journal of Plant Pathology, 92(4 Supplement), S115.

    Google Scholar 

  • Bogoutdinov, D. Z. (2000). Phytoplasma diseases of potato and methods of their study. Samara: Samara State Agricultural Academy (In Russian).

    Google Scholar 

  • Bogoutdinov, D. Z. (2006). Evolutionary aspects of appearance of phytoplasma diseases in nature. Proceedings of the Whorkshop on Plant Virus Diseases. (devoted to the 75-year anniversary of Yu.I. Vlasov) (pp. 44–51). St-Petersburg-Pushkin: VIZR. (In Russian).

  • Bogoutdinov, D. Z., Valiunas, D., Navalinskene, M., & Samuitene, M. (2008). Identification of phytoplasma species, causative agents of solanaceae crop diseases. Agricultural Biology, 1, 77–80 (In Russian).

    Google Scholar 

  • Deng, S., & Hiruki, C. (1991). Amplification of 16S rRNA genes from culturable and non-culturable mollicutes. Journal of Microbiological Methods, 14, 53–61.

    Article  CAS  Google Scholar 

  • Ember, I., Acs, Z., Munyaneza, J. E., Crosslin, J. M., & Kolber, M. (2011). Survey and molecular detection of phytoplasmas associated with potato in Romania and southern Russia. European Journal of Plant Pathology, 130, 367–377.

    Article  Google Scholar 

  • Eristavi, E. M., Sakvaremidze, N. A., & Titvinidze, S. S. (1952). Tomato stolbur in Georgia. Works of Institute for Plant Protection of Georgian SSR Academy of Science, 8, 111–141 (In Russian).

    Google Scholar 

  • Girsova, N., Bottner, K. D., Mozhaeva, K. A., Kastalyeva, T. B., Owens, R. A., & Lee, I.-M. (2008). Molecular detection and identification of group 16SrI and 16SrXII phytoplasmas associated with diseased potatoes in Russia. Plant Disease, 92(4), 654.

    Article  Google Scholar 

  • Goldin, M. I., & Bagdasaryan, A. E. (1969). Tomato stolbur in Azerbaijan. Plant Protection, 2, 22–23 (In Russian).

    Google Scholar 

  • Goldin, M. I., & Parievskaya, A. P. (1950). Materials on the stolbur of tomatoes in the Crimea. Microbiology, 19, 527–531 (In Russian).

    CAS  Google Scholar 

  • Goldin, M. I., & Yurchenko, M. A. (1961). Tomato stolbur and some other virus diseases look like yellows in Alma-Ata Region. Works of Institute for Microbiology and Virology of Kazakh SSR Academy of Science, 5, 139–147 (In Russian).

    Google Scholar 

  • Green, M. J., Thompson, D. A., & MacKenzie, D. J. (1999). Easy and efficient DNA extraction from woody plants for the detection of phytoplasmas by polymerase chain reaction. Plant Disease, 83, 482–485.

    Article  CAS  Google Scholar 

  • Guclu, S., & Ozbek, H. (1991). Studies on the vectors of MLOs caused stolbur disease of potatoes in Erzurum. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 22, 35–42.

    Google Scholar 

  • Gundersen, D. E., & Lee, I.-M. (1996). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea, 35, 144–151.

    CAS  Google Scholar 

  • Isamukhamedov, M. Z. (1969). Stolbur of Solanaceae in Uzbekistan. Uzbek Biological Journal, 3, 13–15 (In Russian).

    Google Scholar 

  • Jakovljević, M., Jović, J., Mitrović, M., Krstić, O., Kosovac, A., Toševski, I., & Cvrković, T. (2015). Euscelis incisus (Cicadellidae, Deltocephalinae), a natural vector of 16DrIII-B phytoplasma causing multiple inflorescence disease of Cirsium arvense. Annals of Applied Biology. doi:10.1111/aab.12236.

    Google Scholar 

  • Khachatryan, G. A. (1950). Potato stolbur wilt in Armenia and its transmission by grafting. News of Academy of Sciences of Armenian SSR, 3, 1113–1120 (In Russian).

    Google Scholar 

  • Korachevskiy, I. K. (1936). Stolbur disease of plants. In M. S. Dunin (Ed.), Virus diseases of plants, 5 (pp. 99–111). Moscow: VASKHNIL (In Russian).

    Google Scholar 

  • Korachevskiy, I. K., & Semenkova, A. V. (1938). On the susceptibility of solanaceae and convolvulaceae plants to big bud disease. Plant Virus Diseases., 2, 118–124 (In Russian).

    Google Scholar 

  • Lee, I.-M., Hammond, R. W., Davis, R. E., & Gundersen, D. E. (1993). Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. Phytopathology, 83, 834–842.

    Article  CAS  Google Scholar 

  • Lee, I.-M., Gundersen-Rindal, D. E., Davis, R. E., & Bartoszyk, I. M. (1998). Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology, 48, 1153–1169.

    Article  CAS  Google Scholar 

  • Lee, I.-M., Davis, R. E., & Gundersen-Rindal, D. E. (2000). Phytoplasma: phytopathogenic mollicutes. Annual Review of Microbiology, 54, 221–555.

    Article  CAS  PubMed  Google Scholar 

  • Lee, I.-M., Bottner, K. D., Munyaneza, J. E., Secor, G. A., & Gudmestad, N. C. (2004a). Clover proliferation group (16SrVI) subgroup A (16SrVI-A) phytoplasma is a probable causal agent of potato purple top disease in Washington and Oregon. Plant Disease, 88, 429.

    Google Scholar 

  • Lee, I.-M., Gundersen-Rindal, D. E., Davis, R. E., Bottner, K. D., Marcone, C., & Seemüller, E. (2004b). ‘Candidatus Phytoplasma asteris’, a novel phytoplasma taxon associated with aster yellows and related disease. International Journal of Systematic and Evolutionary Microbiology, 54, 1037–1048.

    Article  CAS  PubMed  Google Scholar 

  • Lee, I.-M., Martini, M., Marcone, C., & Zhu, S. F. (2004c). Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of ‘Candidatus phytoplasma ulmi’ for the phytoplasma associated with elm yellows. International Journal of Systematic and Evolutionary Microbiology, 54(Pt 2), 337–47.

    Article  CAS  PubMed  Google Scholar 

  • Lee, I.-M., Bottner, K. D., Munyaneza, J. E., Davis, R. E., Crosslin, J. M., du Toit, L. J., & Crosby, T. (2006). Carrot purple leaf: a new spiroplasmal disease associated with carrots in Washington State. Plant Disease, 90(8), 989–993.

    Article  CAS  Google Scholar 

  • Mikec, I., Križanac, I., Budinščak, Ž., Šeruga, M. M., Krajačić, M., & Škorić, D. (2006). Phytoplasmas and their potential vectors in vineyards of indigenous Croatian varieties. In Extended abstracts 15th meeting of the ICVG (pp. 255–257). Stellenbosch: South African Society for Enology and Viticulture.

    Google Scholar 

  • Milkus, B., Clair, D., Idir, S., Habili, N., & Boudon-Padieu, E. (2005). First detection of stolbur phytoplasms in grapevines (Vitis vinifera cv. Chardonnay) affected with grapevine yellows in the Ukraine. Plant Pathology, 54(2), 236.

    Article  Google Scholar 

  • Najet, N., Vadamalai, G., Davis, R. E., Harrison, N. A., Sijam, K., Dickinson, M., Abdullah, S. N. A., & Zhao, Y. (2013). ‘Candidatus phytoplasma malaysianum’, a novel taxon associated with virescence and phyllody of Madagascar periwinkle (Catharanthus roseus). International Journal of Systematic and Evolutionary Microbiology, 63, 540–548.

    Article  Google Scholar 

  • Poner, V. M. (1941). On varietal susceptibility of Solanaceae to stolbur (big bud) disease. In V. L. Ryzhkov (Ed.), Proceedings of the workshop ‘plant virus diseases and their control’ (pp. 245–254). Moscow-Leningrad: Academy of Science of the USSR (In Russian).

    Google Scholar 

  • Protsenko, А. Е. (1974). Areal of plant viruses and phytopathogenic mycoplasmas and their control. Works of All-Union Institute for Plant Protection, 41, 7–12 (In Russian).

    Google Scholar 

  • Razvyazkina, G. M. (1950). On the dissemination of the stolbur virus in the wild. Microbiology, 19, 256–259 (In Russian).

    Google Scholar 

  • Ryzhkov, V. L., & Korachevskiy, I. K. (1934). Experiments on the artificial transmission of virus diseases of the tomato. In V. L. Ryzhkov (Ed.), Virus diseases of plants in the Crimea and the Ukraine (pp. 7–30). Simferopol: State Publishing House of Crimean ASSR (In Russian).

    Google Scholar 

  • Samsonova, L. N. (1979). Mycoplasma-like nature of potato witches’-broom. In Y. I. Vlasov (Ed.), The works of the Russian research institute for plant protection (pp. 43–47). Leningrad: VIZR (In Russian).

    Google Scholar 

  • Samsonova, L. N., & Bogoutdinov, D. Z. (1993). Role of mixed infections in tomato stolbur disease. In Y. I. Vlasov (Ed.), Viral epidemics and ways to prevent them (pp. 49–54). St. Petersburg: VIZR (In Russian).

    Google Scholar 

  • Samsonova, L. N., Larina, E.I., & Gerasenkova, Е. А. (1979). Damage of potato by mycoplasma-like disease in Leningradskaya oblast. Ibid. (pp. 48–51). (In Russian).

  • Samsonova, L. N., Vlasov, Y. I., & Bogoutdinov, D. Z. (1991). About complex study of mycoplasma-like disease (methodology). St. Petersburg: VIZR (In Russian).

    Google Scholar 

  • Samudzheva, E. M. (1949). Some data on study of tomato stolbur vectors in Georgia. Works of Institute for Plant Protection of Georgian SSR Academy of Science, 6, 161–162 (In Russian).

    Google Scholar 

  • Samudzheva, E. M. (1953). Study of biological properties of stolbur vector, leafhopper Hyalesthes mlokosiewiczi Sign. Works of Institute for Plant Protection of Georgian SSR Academy of Science, 9, 15–28 (In Russian).

    Google Scholar 

  • Schneider, B., Ahrens, U., Kirkpatrick, B. C., & Seemüller, E. (1993). Classification of plant-pathogenic mycoplasma-like organisms using restriction-site analysis of PCR amplified 16S rDNA. Journal of General Microbiology, 139, 519–527.

    Article  CAS  Google Scholar 

  • Serbinov, B. I. (1941). Measures for tomato stolbur disease control in Moldavian SSR. In V. L. Ryzhkov (Ed.), Proceedings of the workshop ‘plant virus diseases and their control’ (pp. 264–268). Moscow-Leningrad: Academy of Science of the USSR (In Russian).

    Google Scholar 

  • Sukhov, K. C., & Razvyazkina, G. M. (1955). Biology of viruses and virus diseases of plants. Moscow: Soviet Science.

    Google Scholar 

  • Sukhov, K. C., & Vovk, A. M. (1946a). Leafhopper Hyalesthes obsoletus as a vector of stolbur of solanaceae. Reports of USSR Academy of Science, 53(2), 153–156 (In Russian).

    Google Scholar 

  • Sukhov, K. C., & Vovk, A. M. (1946b). Potato stolbur. Reports of All-Union Academy of Agricultural Sciences., 1–2, 24–29 (In Russian).

    Google Scholar 

  • Sukhov, K. C., & Vovk, A. M. (1947). Virus of stolbur as a causative agent of mass wilt of pepper, eggplant and potato in the South. Reports of USSR Academy of Science, 58(2), 319–321 (In Russian).

    Google Scholar 

  • Sukhov, K. C., & Vovk, A. M. (1949). Stolbur of solanaceae. Мoscow-Leningrad: USSR Academy of Science (In Russian).

    Google Scholar 

  • Sukhov, K. C., & Vovk, A. M. (1950). Distinguishing between north and south potato stolbur. The Works of Institute of Genetics of USSR Academy of Science, 17, 236–238 (In Russian).

    Google Scholar 

  • Tanne, E., Boudon-Padieu, E., Clair, D., Davidovich, M., Melamed, S., & Meir, K. (2001). Detection of phytoplasma by polymerase chain reaction of insect feeding medium and its use in determining vectoring ability. Phytopathology, 91, 741–746.

    Article  CAS  PubMed  Google Scholar 

  • Tsyplenkov, A. E., & Fedotina, V. L. (1973). About nature and classification of two forms of stolbur. Biological Sciences, 5, 100–106 (In Russian).

    Google Scholar 

  • Vlasov, Y. I., Larina, E. I., & Teploukhova, T. N. (1972). Tomato virus disease in Central Asia and their control. Bulletin of All-Union Institute for Plant Protection, 24, 58–62 (In Russian).

    Google Scholar 

  • Vlasov, Y. I., Samsonova, L. N., & Bogoutdinov, D. Z. (1992). Contemporary view on tomato stolbur circulation in Chechnya and Ingushetiya. Reports of All-Union Agricultural Academy of Sciences., 4, 23–24 (In Russian).

    Google Scholar 

  • Wei, W., Davis, R. E., Lee, I.-M., & Zhao, Y. (2007). Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. International Journal of Systematic and Evolutionary Microbiology, 57, 1855–1867.

    Article  CAS  PubMed  Google Scholar 

  • Yachevskiy, A. A. (1926). Potato witches’-broom. Materials on Mycology and Phytopathology, 5(2), 1–12 (In Russian).

    Google Scholar 

  • Yemelyanov A. F. (1964). Suborder Cicadinea (Auchenorrhyncha). In: The Determinant of Insects in the European Part of the USSR. 1, 337–437. (In Russian).

  • Zhao, Y., Wei, W., Lee, I.-M., Shao, J., Suo, X., & Davis, R. E. (2009). Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology, 59, 2582–2593.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

This research was funded under the ISTC Russia-US collaborative project #3468.

Author information

Authors and Affiliations

  1. Russian Research Institute of Phytopathology, 143050, Bolshie Vyaziomy, Moscow oblast, Russia

    Natalia V. Girsova, Yuri I. Meshkov, Karina A. Mozhaeva & Tatyana B. Kastalyeva

  2. USDA/ARS, Molecular Plant Pathology Laboratory, Beltsville, MD, USA

    Kristi D. Bottner-Parker & Ing Ming Lee

  3. Samara State Agricultural Academy, 446442, Kinel, Samara oblast, Russia

    Damir Z. Bogoutdinov

Authors
  1. Natalia V. Girsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kristi D. Bottner-Parker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Damir Z. Bogoutdinov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuri I. Meshkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Karina A. Mozhaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tatyana B. Kastalyeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ing Ming Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Tatyana B. Kastalyeva or Ing Ming Lee.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(DOCX 12 kb)

ESM 2

(DOCX 14 kb)

ESM 3

(DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Girsova, N.V., Bottner-Parker, K.D., Bogoutdinov, D.Z. et al. Diverse phytoplasmas associated with potato stolbur and other related potato diseases in Russia. Eur J Plant Pathol 145, 139–153 (2016). https://doi.org/10.1007/s10658-015-0824-3

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-015-0824-3

Keywords

Search

Navigation