Advertisement

European Journal of Plant Pathology

, Volume 145, Issue 1, pp 103–118 | Cite as

Characterization of Cylindrodendrum, Dactylonectria and Ilyonectria isolates associated with loquat decline in Spain, with description of Cylindrodendrum alicantinum sp. nov.

  • Carlos Agustí-Brisach
  • Ana Cabral
  • Elisa González-Domínguez
  • Ana Pérez-Sierra
  • Maela León
  • Paloma Abad-Campos
  • José García-Jiménez
  • Helena Oliveira
  • Josep ArmengolEmail author
Article

Abstract

Thirty-one loquat orchards (Eriobotrya japonica ‘Algerie’) with plants exhibiting decline symptoms were surveyed between 2004 and 2007 in the province of Alicante, Spain. Twenty-eight representative isolates with Cylindrocarpon-like asexual morphs recovered from affected roots were included in this study, with the objective to characterize them by means of phenotypical characterization, DNA analysis and pathogenicity tests. Dactylonectria alcacerensis, D. torresensis and Ilyonectria robusta were identified based on morphological and cultural characteristics as well as DNA sequence data for part of histone H3, with D. torresensis the most frequent species. All of them are reported for the first time on loquat, and I. robusta is reported for the first time in Spain. In addition, one species is newly described, Cylindrodendrum alicantinum. Pathogenicity tests with representative isolates showed that these species were able to induce typical root rot disease symptoms, affecting plant development or even leading to plant death. This research demonstrates the association of species belonging to the genera Cylindrodendrum, Dactylonectria and Ilyonectria with root rot of loquat and loquat decline in the province of Alicante (eastern Spain). This information should be considered for the improvement of the current management strategies against these soil-borne pathogens when establishing new loquat plantations or introducing new susceptible fruit crops in the region.

Keywords

Cylindrodendrum alicantinum Dactylonectria alcacerensis D. torresensis Eriobotrya japonica Ilyonectria robusta loquat decline 

Notes

Acknowledgments

We acknowledge Dr. L. Lombard and Prof. Dr. P.W. Crous (CBS-KNAW Fungal Biodiversity Centre, The Netherlands) for valuable discussions and data sharing. This work was funded by the Cooperativa Agrícola de Callosa d’En Sarrià (Alicante, Spain). We would like to thank E. Soler for their technical assistance.

References

  1. Agustí-Brisach, C., & Armengol, J. (2013). Black-foot disease of grapevine: an update on taxonomy, epidemiology and management strategies. Phytopathologia Mediterranea, 52, 245–261.Google Scholar
  2. Agustí-Brisach, C., Gramaje, D., García-Jiménez, J., & Armengol, J. (2013a). Detection of Ilyonectria spp. in the grapevine nursery propagation process in Spain. European Journal of Plant Pathology, 137, 103–112.CrossRefGoogle Scholar
  3. Agustí-Brisach, C., Gramaje, D., García-Jiménez, J., & Armengol, J. (2013b). Detection of black-foot and Petri disease pathogens in natural soils of grapevine nurseries and vineyards using bait plants. Plant and Soil, 364, 5–13.CrossRefGoogle Scholar
  4. Aiello, D., Guarnaccia, V., Vitale, A., Cirvilleri, G., Granata, G., Epifani, F., Perrone, G., Polizzi, G., Groenewald, J. Z., & Crous, P. W. (2014). Ilyonectria palmarum sp. nov. causing dry basal stem rot of Arecaceae. European Journal of Plant Pathology, 138, 347–359.CrossRefGoogle Scholar
  5. Booth, C. D. (1966). The genus Cylindrocarpon. Mycological Papers (CMI), 104, 1–56.Google Scholar
  6. Brayford, D. (1993). Cylindrocarpon. In L. L. Singleton, J. D. Mihail, & C. M. Rush (Eds.), Methods for research on soilborne phytopathogenic fungi (pp. 103–106). St. Paul: APS Press.Google Scholar
  7. Cabral, A., Groenewald, J. Z., Rego, C., Oliveira, H., & Crous, P. W. (2012a). Cylindrocarpon root rot: multi-gene analysis reveals novel species within the Ilyonectria radicicola species complex. Mycological Progress, 11, 655–688.CrossRefGoogle Scholar
  8. Cabral, A., Rego, C., Nascimento, T., Oliveira, H., Groenewald, J. Z., & Crous, P. W. (2012b). Multi-gene analysis and morphology reveal novel Ilyonectria species associated with black foot disease of grapevines. Fungal Biology, 116, 62–80.CrossRefPubMedGoogle Scholar
  9. Calabrese, F. (2006). Origen de la especie. In M. Agustí, C. Reig, & P. Undurraga (Eds.), El cultivo del níspero japonés. España: Pontificia Universidad Católica de Valparaíso, Chile and Universidad Politécnica de Valencia.Google Scholar
  10. Chaverri, P., Salgado, C., Hirooka, Y., Rossman, A. Y., & Samuels, G. J. (2011). Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon-like anamorphs. Studies in Mycology, 68, 57–78.CrossRefPubMedPubMedCentralGoogle Scholar
  11. Crous, P. W., Gams, W., Stalpers, J. A., Robert, V., & Stegehuis, G. (2004a). MycoBank: an online initiative to launch mycology into the 21st century. Studies in Mycology, 50, 19–22.Google Scholar
  12. Crous, P. W., Groenewald, J. Z., Risede, J. M., & Hywel-Jones, N. L. (2004b). Calonectria species and their Cylindrocladium anamorphs: species with sphaeropedunculate vesicles. Studies in Mycology, 50, 415–429.Google Scholar
  13. Crous, P.W., Verkleij, G.J.M., Groenewald, J.Z., Samson, R.A. (Eds.) (2009). Fungal biodiversity. CBS laboratory manual series 1. Centraalbureau voor Schimmelcultures, Utrecht.Google Scholar
  14. Dhingra, O. D., & Sinclair, J. B. (1995). Basic plant pathology methods (2nd ed.). Boca Raton: CRC Press.Google Scholar
  15. Erper, I., Agustí-Brisach, C., Tunali, B., & Armengol, J. (2013). Characterization of root rot disease of kiwifruit in the Black Sea region of Turkey. European Journal of Plant Pathology, 136, 291–300.CrossRefGoogle Scholar
  16. Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.CrossRefGoogle Scholar
  17. Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basiodiomycetes-applications to the identification of mycorrhizae and rusts. Molecular Ecology, 2, 113–118.CrossRefPubMedGoogle Scholar
  18. Glass, N. L., & Donaldson, G. (1995). Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology, 61, 1323–1330.PubMedPubMedCentralGoogle Scholar
  19. González-Domínguez, E., Pérez-Sierra, A., Álvarez, L. A., Abad-Campos, P., Armengol, J., & García-Jiménez, J. (2008). Ethiology of decline of loquat (Eriobotrya japonica) in eastern Spain. Journal of Plant Pathology, 90(2, supplement), 179.Google Scholar
  20. González-Domínguez, E., Pérez-Sierra, A., Álvarez, L. A., León, M., Abad-Campos, P., Armengol, J., & García-Jiménez, J. (2009). Agentes fúngicos presentes en plantaciones de nísperos (Eriobotrya japonica Lindl.) con síntomas de decaimiento en la provincia de Alicante. Boletín Sanidad Vegetal Plagas, 35, 453–467.Google Scholar
  21. González-Domínguez, E., Rossi, V., Armengol, J., & García-Jiménez, J. (2013). Effect of environmental factors on mycelial growth and conidial germination of Fusicladium eriobotryae, and the infection of loquat leaves. Plant Disease, 97, 1331–1338.CrossRefGoogle Scholar
  22. González-Domínguez, E., Armengol, J., & Rossi, V. (2014). Development and validation of a weather-based model for predicting infection of loquat fruit by Fusicladium eriobotryae. Plos One, 9, e107547.CrossRefPubMedPubMedCentralGoogle Scholar
  23. Halleen, F., Schroers, H. J., Groenewald, J. Z., & Crous, P. W. (2004). Novel species of Cylindrocarpon (Neonectria) and Campylocarpon gen. nov. associated with black-foot disease of grapevines (Vitis spp). Studies in Mycology, 50, 431–455.Google Scholar
  24. Halleen, F., Fourie, P. H., & Crous, P. W. (2006). A review of black-foot disease of grapevine. Phytopathologia Mediterranea, 45, S55–S67.Google Scholar
  25. Janick, J. (2011). Predictions for loquat improvement in the next decade. Acta Horticulturae, 887, 25–30.CrossRefGoogle Scholar
  26. Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J., & Higgins, D. G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23, 2947–2948.CrossRefPubMedGoogle Scholar
  27. Lin, S. Q. (2007). World loquat production and research with special reference to China. Acta Horticulturae, 750, 37–44.CrossRefGoogle Scholar
  28. Lombard, L., Van der Merwe, N. A., Groenewald, J. Z., & Crous, P. W. (2014). Lineages in Nectriaceae: Re-evaluating the generic status of Ilyonectria and allied genera. Phytopathologia Mediterranea, 53, 515–532.Google Scholar
  29. Nirenberg, H. (1976). Untersuchungen über die morphologische und biologische Differenzierung in der Fusarium-Sektion Liseola. Mitteilungen aus der Biologischen Bundesanstalt für Land- und Forstwirtschaft, 169, 1–117.Google Scholar
  30. O’Donnell, K., & Cigelnik, E. (1997). Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution, 7, 103–116.CrossRefPubMedGoogle Scholar
  31. Petit, E., & Gubler, W. D. (2005). Characterization of Cylindrocarpon species, the cause of black foot disease of grapevine in California. Plant Disease, 89, 1051–1059.CrossRefGoogle Scholar
  32. Rayner, R. W. (1970). A mycological colour chart. Kew: British Mycological Society and CAB International Mycological Institute.Google Scholar
  33. Read, N. D., Lichius, A., Shoji, J. Y., & Goryachev, A. B. (2009). Self-signalling and self-fusion in filamentous fungi. Current Opinion in Microbiology, 12, 608–615.CrossRefPubMedGoogle Scholar
  34. Reig, C., Farina, V., Volpe, G., Mesejo, C., Martínez-Fuentes, A., Barone, F., Calabrese, F., & Agustí, M. (2012). Giberellic acid and flower bud development in loquat (Eriobotrya japonica Lindl.). Scientia Horticulturae, 129, 27–31.CrossRefGoogle Scholar
  35. Samuels, G. J., & Brayford, D. (1990). Variation in Nectria radicicola and its anamorph, Cylindrocarpon destructans. Mycological Research, 94, 433–442.CrossRefGoogle Scholar
  36. Sánchez-Hernández, M. E., Ruiz-Dávila, A., Pérez de Algaba, A., Blanco-López, M. A., & Trapero-Casas, A. (1998). Occurrence and etiology of death of young olive tres in southern Spain. European Journal of Plant Pathology, 104, 347–357.CrossRefGoogle Scholar
  37. Sánchez-Torres, P., Hinarejos, R., & Tuset, J. J. (2009). Characterization and pathogenicity of Fusicladium eriobotryae, the fungal pathogen responsible for loquat scab. Plant Disease, 93, 1151–1157.CrossRefGoogle Scholar
  38. Schroers, H. J., Zerjav, M., Munda, A., Halleen, F., & Crous, P. W. (2008). Cylindrocarpon pauciseptatum sp. nov., with notes on Cylindrocarpon species with wide, predominantly 3-septate macroconidia. Mycological Research, 112, 82–92.CrossRefPubMedGoogle Scholar
  39. Soler, E., Martínez-Calvo, J., Llácer, G., & Badenes, M. L. (2007). Loquat in Spain: production and marketing. Acta Horticulturae, 750, 45–47.CrossRefGoogle Scholar
  40. Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary 16 genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.CrossRefPubMedPubMedCentralGoogle Scholar
  41. Tewoldemedhin, Y. T., Mazzola, M., Mostert, L., & McLeod, A. (2011). Cylindrocarpon species associated with apple tree roots in South Africa and their quantification using real-time PCR. European Journal of Plant Pathology, 129, 637–651.CrossRefGoogle Scholar
  42. Vitale, A., Aiello, D., Guarnaccia, V., Perrone, G., Stea, G., & Polizzi, G. (2012). First report of root rot caused by Ilyonectria (=Neonectria) macrodidyma on avocado (Persea americana) in Italy. Journal of Phytopathology, 160, 156–159.CrossRefGoogle Scholar
  43. Zhuang, W. Y., Nong, Y., & Luo, J. (2007). New species and new Chinese records of Bionectriaceae and Nectriaceae (Hypocreales, Ascomycetes) from Hubei, China. Fungal Diversity, 24, 347–357.Google Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2015

Authors and Affiliations

  • Carlos Agustí-Brisach
    • 1
  • Ana Cabral
    • 2
  • Elisa González-Domínguez
    • 1
    • 3
  • Ana Pérez-Sierra
    • 1
  • Maela León
    • 1
  • Paloma Abad-Campos
    • 1
  • José García-Jiménez
    • 1
  • Helena Oliveira
    • 2
  • Josep Armengol
    • 1
    Email author
  1. 1.Instituto Agroforestal MediterráneoUniversidad Politécnica de ValenciaValenciaSpain
  2. 2.Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de AgronomiaUniversidade de LisboaLisbonPortugal
  3. 3.Department of Sustainable Crop Production - DI.PRO.VE.S., Facoltà di Scienze agrarie, alimentari e ambientaliUniversità Cattolica del Sacro CuorePiacenzaItaly

Personalised recommendations