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European Journal of Plant Pathology

, Volume 144, Issue 2, pp 399–410 | Cite as

Cucurbit powdery mildews: methodology for objective determination and denomination of races

  • Aleš LebedaEmail author
  • Eva Křístková
  • Božena Sedláková
  • James D. McCreight
  • Michael D. Coffey
Article

Abstract

Cucurbit powdery mildew (CPM), a disease on field and greenhouse cucurbit crops worldwide, is caused most frequently by two obligate erysiphaceous ectoparasites (Golovinomyces orontii s.l., Podosphaera xanthii) that are highly variable in their pathogenicity and virulence. Various independent systems of CPM race determination and denomination are used worldwide, having to date been differentiated on different cultivars or lines of melon (Cucumis melo L.). We briefly review historical perspectives and the current state of understanding of the virulence variation of the two CPM pathogens at the pathogenic race level, their differentiation and their designation. Furthermore, we propose for use by the international CPM research, breeding, seed and production community new tools to enhance research, communication and management of CPM. These tools consist of five components: 1) a set of 21 differential genotypes of Cucumis melo L. for the identification of CPM races; 2) a triple-part, septet code for meaningful, concise designation of CPM races; 3) protocols for maintaining CPM isolates and differential genotypes and for laboratory assays to examine the virulence of CPM isolates; 4) rules and principles of practical application of this system in breeding, seed production and cucurbit growing, including a proposal of a race denomination suitable for practical application; and 5) crucial activities leading to the implementation and running of new tools for CPM research and management. The five components of this package have equal importance, forming a compact system, and none of them can be omitted.

Keywords

Golovinomyces orontii s.l. Podosphaera xanthii Cucumis melo Virulence variability Race-specificity Septet code 

Notes

Acknowledgments

This research was supported by the following grants: 1) MSM 6198959215 (Czech Ministry of Education); 2) QH71229 (NAZV, National Agency for Agricultural Research of the Czech Republic); 3) Internal Grant Agency Palacký University in Olomouc (Prf_2015_001).

Compliance with ethical standards

Conflict of interest

There are no potential conflicts (financial or non-financial) of interest.

Human and animal rights

Research did not involve Human Participants and/or Animals

Sources of funding

1) MSM 6198959215 (Czech Ministry of Education);2) QH71229 (NAZV, National Agency for Agricultural Research of the Czech Republic); 3) Internal Grant Agency Palacký University in Olomouc (Prf_2015_001).

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2015

Authors and Affiliations

  • Aleš Lebeda
    • 1
    Email author
  • Eva Křístková
    • 1
  • Božena Sedláková
    • 1
  • James D. McCreight
    • 2
  • Michael D. Coffey
    • 3
  1. 1.Faculty of Science, Department of BotanyPalacký UniversityOlomouc-HoliceCzech Republic
  2. 2.U.S. Department of Agriculture, Agricultural Research ServiceSalinasUSA
  3. 3.Department of Plant Pathology and MicrobiologyUniversity of CaliforniaRiversideUSA

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