Phenotypic and genotypic diversity of Pectobacterium carotovorum subsp carotovorum causing soft rot disease of potatoes in Morocco
- 248 Downloads
The species Pectobacterium carotovorum include a diverse subspecies of bacteria that cause disease on a wide variety of plants. In Morocco, approximately 95 % of the P. carotovorum isolates from potato plants with tuber soft rot are P. carotovorum subsp carotovorum. The aim was to monitor this Moroccan population in time and to continue the work carried out to understand the genetic diversity of these subspecies. The diversity of 30 strains of P. carotovorum subsp carotovorum collected between 2012 and 2013 from potato soft rot from different Moroccan regions was evaluated. On the basis of phenotypic characteristics, the isolates were classified into four different biovars and in three groups based on the aggressiveness traits. Moreover, the genetic variability was also investigated by fingerprint analysis. The typing by ERIC and REP sequences of the new collection compared to ERIC-PCR and phylogenetic analysis of pmrA sequences, demonstrated that there is a considerable genetic diversity in P. carotovorum subsp. carotovorum strains, which can be divided into two distinct groups, independently to their source of isolation, by their pathogenicity.
KeywordsSoft rot potato Pectobacterium carotovorum subsp carotovorum rep-PCR Biochemical characteristics Pathogenicity Genetic diversity
This work was supported by project ARIMNET PoHMed (Potato Health – Managed for Efficacy and Durability) from Coordination of the Agricultural Research in the Mediterranean Area and Moroccan cooperation. The authors wish to thank sir Rachid Tahziman for his valuable assistance.
Compliance with ethical standards
Authors FH and AM have received a research fellowship from project ARIMNET PoHMed (Potato Health – Managed for Efficacy and Durability)
The authors declare that they have no competing interests.
- Achbani, E., Maazouz, H., & Boulif, M. (1993). Inventaire et importance des maladies fongiques et bactériennes de la pomme de terre dans la région de Meknès. Al AWAMIA, 80, 82–83.Google Scholar
- Anita, N. S., Andreas, R., & Harald, H. (2005). Specificity of enterobacterial repetitive intergenic consensus and repetitive extragenic palindromic polymerase chain reaction for the detection of clonality within the Enterobacter cloacae complex. Diagnostic Microbiology and Infectious Disease, 53, 9–16.CrossRefGoogle Scholar
- Dye, D. W. (1969). A taxonomic study of the genus Erwinia. II. The “carotovora” group. New Zealand Journal of Science, 12, 81–97.Google Scholar
- Frank, J. L., Dennis, W. F., Christine, T. S., & Frans, J. B. (1994). Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequencesand PCR. Applied and Environmental Microbiology, 60(7), 2286–2295.Google Scholar
- Gallelli, A., Galli, M., De Simone, D., Zaccardelli, M., & Loreti, S. (2009). Phenotypic and genetic variability of Pectobacterium carotovorum isolated from artichoke in the Sele valley. Journal of Plant Pathology, 91, 757–761.Google Scholar
- Gardan, L., Gouy, C., Christen, R., & Samson, R. (2003). Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. International Journal of Systematic and Evolutionary Microbiology, 53, 381–391.CrossRefPubMedGoogle Scholar
- Kettani-Halabi, M., Terta, M., Amdan, M., Elfahime, E., Bouteau, F., & Ennaji, M. M. (2013). An easy, simple inexpensive test for the specific detection of Pectobacterium carotovorum subsp. carotovorum based on sequence analysis of the pmrA gene. BMC Microbiology, 13, 176.PubMedCentralCrossRefPubMedGoogle Scholar
- Muiru, W. M., Koopmann, B., Tiedemann, A. V., Mutitu, E. W., & Kimenju, J. W. (2010). Use of Repetitive Extragenic Palindromic (REP), Enterobacterial Repetitive Intergenic Consensus (ERIC) and BOX sequences to fingerprint Exserohilum turcicum isolates. Journal of Applied Biosciences, 30, 1828–1338.Google Scholar
- Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: A laboratory manual (2nd ed.). New York (USA): Cold Spring Harbor Laboratory Press.Google Scholar
- Samson, R., Legendre, J. B., Christen, R., Fischer-Le Saux, M., Achouak, W., & Gardan, L. (2005). Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. International Journal of Systematic and Evolutionary Microbiology, 55, 1415–1427.CrossRefPubMedGoogle Scholar
- Terta, M., El Karkouri, A., Ait M'hand, R., Achbani, E., Barakate, M., Amdan, M., Annajar, B., Ennaji, M. M., et al. (2010). Occurrence of Pectobacterium carotovorum Strains isolated from potato soft rot in Morocco. Cellular and Molecular Biology, 56, 1324–1333.Google Scholar
- Terta, M., Azelmat, S., Ait M’hand, R., Achbani, E., Barakate, M., Bouteau, F., & Ennaji, M. M. (2012). Molecular typing of Pectobacterium carotovorum isolated from potato tuber soft rot in Morocco. Annals of Microbiology, 7, 1–7.Google Scholar
- Vitale, S., Alberino, S., Zoina, A., Parisi, B., & Corazza, L. (2004). Evaluation of resistance to dry and soft rot of potato clones adapted to Mediterranean regions. Phytopathologia Polonica, 34, 13–20.Google Scholar
- Zulkifli, Y., Alitheen, N. B., Son, R., Raha, A. R., Samuel, L., Yeap, S. K., & Nishibuchi, M. (2009). Random amplified polymorphic DNA-PCR and ERIC-PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. Food Research International Journal, 16, 141–150.Google Scholar