Phenotypic and genotypic responses of chili (Capsicum annuum L.) progressive lines with different resistant genes against anthracnose pathogen (Colletotrichum spp.)
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Anthracnose disease caused by Colletotrichum spp. is a serious disease of chili (Capsicum annuum), particularly in tropical countries. Host plant resistance breeding is one of the most effective disease management strategies. It requires identification of parents with genes resistant to Colletotrichum species predominant in a given region. For phenotypic evaluation, 35 chili lines consisting of progressive lines of C. annuum derived from C. baccatum PBC80 (34 lines) and from C. chinense PBC932 (one line) were inoculated with two aggressive isolates of Colletotrichum acutatum (Ca) and C. capsici (Cc) by injection into the pericarp of green and red-ripe fruit. Lesion diameters were scored at seven days after inoculation. Two simple sequence repeat (SSR) and one sequence characterized amplified region (SCAR) markers were used for validation in four progressive lines and three each of susceptible and resistant checks for genotypic response. The progressive lines were classified into 10 groups based on their responses to two pathogens and at the two fruit stages. Four progressive lines (101, 205, 210 and 215) were selected and used for developing crosses to combine resistance genes from two sources resistant to Ca and Cc at both fruit stages. Progressive lines derived from PBC80 showed DNA fragment 231 bp amplified by primer HpmsE032 associated with Cc at green fruit stages. Hence the HpmsE032 marker could be considered useful in the selection of resistant genotypes derived from PBC80.
KeywordsDNA marker Fruit rot Hot pepper Pathogenicity Resistance
The authors are grateful to The Royal Golden Jubilee (RGJ) Ph.D. Program, Thailand Research Fund (TRF); The National Science and Technology Agency (NSTDA); National Research University Project of Thailand, Office of the Higher Education Commission, through the Food and Functional Food Research Cluster of Khon Kaen University; Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University for funding the research, Thailand Research Fund(TRF) (Project code : IRG5780003), Khon Kaen University (KKU) and the Faculty of Agriculture KKU for providing financial support for manuscript preparation activities and RGJ fellowship to the first author. The authors are also grateful to AVRDC – The World Vegetable Center, Taiwan and Asst. Prof. Chalermsri Nontaswatsri, Maejo University, Thailand for providing seeds of genotypes.
- Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11–15.Google Scholar
- FAO. (2013). Food and Agriculture Statistics. (2013). (cited 9 May 2015). Available from: http://www.faostat.fao.org.
- Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research. New York: John Wiley and Sons.Google Scholar
- Harp, T. L., Pernezny, K., Lewis Ivey, M. L., Miller, S. A., Kuhn, P. J., & Datnoff, L. (2008). The etiology of recent pepper anthracnose outbreaks in Florida. Crop Protection, 1380–1384.Google Scholar
- Kim, S. H., Yoon, J. B., Do, J. W., & Park, H. G. (2007). Resistance to anthracnose caused by Colletotrichum acutatum in chili pepper (Capsicum annuum L.). Journal of Crop Science and Biotechnology, 10(4), 277–280.Google Scholar
- Kim, S. H., Yoon, J. B., Do, J. W., & Park, H. G. (2008b). Inheritance of anthracnose resistance in a new genetic resource Capsicum baccatum PI594137. Journal of Crop Science and Biotechnology, 11, 13–16.Google Scholar
- Park, K. S., & Kim, C. H. (1992). Identification, distribution, and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean Journal Plant Pathology, 8, 61–69.Google Scholar
- Park, K. S., Kim, S. H., Park, H. G., & Yoon, J. B. (2009). Capsicum germplasm resistance to pepper anthracnose differentially interacts with Colletotrichum isolates. Horticulture Environment and Biotechnology, 50(1), 17–23.Google Scholar
- Poonpolgul, S., & Kumphai, S. (2007). Chili pepper anthracnose in Thailand. First International Symposium. on Chili Anthracnose, Seoul National University, Korea, 23.Google Scholar
- Silva, S. A., Rodrigues, R., Goncalves, L. S. A., Sudre, C. P., Bento, C. S., Carmo, M. G. F., & Medeiros, A. M. (2014). Resistance in Capsicum spp. to anthracnose affected by different stages of fruit development during pre- and post harvest. Tropical Plant Pathology, 39(4), 335–341.CrossRefGoogle Scholar
- Sun, C., Mao, S. L., Zhang, Z. H., Palloix, A., Wang, L. H., & Zhang, B. X. (2015). Resistances to anthracnose (Colletotrichum acutatum) of Capsicum mature green and ripe fruit are controlled by a major dominant cluster of QTLs on chromosome P5. Scientia Horticulturae, 181, 81–88.CrossRefGoogle Scholar
- Wang, Y. W. (2011). Development of Sequence characterized amplified region (SCAR) markers associated with pepper anthracnose (Colletotrichum acutatum) resistance. Master Thesis, Department of Agronomy, National Chiayi University, Taiwan.Google Scholar
- Yoon, J. B., Yang, D. C., Lee, W. P., Ahn, S. Y., & Park, H. G. (2004). Genetic resources resistant to anthracnose in the genus Capsicum. Journal of the Korean Society for Horticultural Science, 45, 318–323.Google Scholar