European Journal of Plant Pathology

, Volume 143, Issue 4, pp 725–736 | Cite as

Phenotypic and genotypic responses of chili (Capsicum annuum L.) progressive lines with different resistant genes against anthracnose pathogen (Colletotrichum spp.)

  • Patcharaporn Suwor
  • Petcharat Thummabenjapone
  • Jirawat Sanitchon
  • Sanjeet Kumar
  • Suchila Techawongstien
Article

Abstract

Anthracnose disease caused by Colletotrichum spp. is a serious disease of chili (Capsicum annuum), particularly in tropical countries. Host plant resistance breeding is one of the most effective disease management strategies. It requires identification of parents with genes resistant to Colletotrichum species predominant in a given region. For phenotypic evaluation, 35 chili lines consisting of progressive lines of C. annuum derived from C. baccatum PBC80 (34 lines) and from C. chinense PBC932 (one line) were inoculated with two aggressive isolates of Colletotrichum acutatum (Ca) and C. capsici (Cc) by injection into the pericarp of green and red-ripe fruit. Lesion diameters were scored at seven days after inoculation. Two simple sequence repeat (SSR) and one sequence characterized amplified region (SCAR) markers were used for validation in four progressive lines and three each of susceptible and resistant checks for genotypic response. The progressive lines were classified into 10 groups based on their responses to two pathogens and at the two fruit stages. Four progressive lines (101, 205, 210 and 215) were selected and used for developing crosses to combine resistance genes from two sources resistant to Ca and Cc at both fruit stages. Progressive lines derived from PBC80 showed DNA fragment 231 bp amplified by primer HpmsE032 associated with Cc at green fruit stages. Hence the HpmsE032 marker could be considered useful in the selection of resistant genotypes derived from PBC80.

Keywords

DNA marker Fruit rot Hot pepper Pathogenicity Resistance 

References

  1. Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11–15.Google Scholar
  2. FAO. (2013). Food and Agriculture Statistics. (2013). (cited 9 May 2015). Available from: http://www.faostat.fao.org.
  3. Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research. New York: John Wiley and Sons.Google Scholar
  4. Harp, T. L., Pernezny, K., Lewis Ivey, M. L., Miller, S. A., Kuhn, P. J., & Datnoff, L. (2008). The etiology of recent pepper anthracnose outbreaks in Florida. Crop Protection, 1380–1384.Google Scholar
  5. Kim, S. H., Yoon, J. B., Do, J. W., & Park, H. G. (2007). Resistance to anthracnose caused by Colletotrichum acutatum in chili pepper (Capsicum annuum L.). Journal of Crop Science and Biotechnology, 10(4), 277–280.Google Scholar
  6. Kim, J. T., Park, S. Y., Choi, W., Lee, Y. H., & Kim, H. T. (2008a). Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea. Plant Pathology, 24(1), 17–23.CrossRefGoogle Scholar
  7. Kim, S. H., Yoon, J. B., Do, J. W., & Park, H. G. (2008b). Inheritance of anthracnose resistance in a new genetic resource Capsicum baccatum PI594137. Journal of Crop Science and Biotechnology, 11, 13–16.Google Scholar
  8. Kim, S. H., Yoon, J. B., Do, J. W., & Park, H. G. (2008c). A major recessive gene associated with anthracnose resistance to Colletotrichum capsici in chilli pepper (Capsicum annuum L.). Breeding Science, 58, 137–141.CrossRefGoogle Scholar
  9. Lee, J., Hong, J. H., Do, J. W., & Yoon, J. B. (2010). Identification of QTLs for resistance to anthracnose to two Colletotrichum species in pepper. Journal of Crop Science and Biotechnology, 13(4), 227–233.CrossRefGoogle Scholar
  10. Lee, J., Jee, H. H., Jae, W. D., & Jae, B. Y. (2011). Development of STS markers linked to the major QTLs for resistance to the pepper anthracnose caused by Colletotrichum acutatum and C. capsici. Horticulture Environment and Biotechnology, 52(6), 596–601.CrossRefGoogle Scholar
  11. Lewis Ivey, M. L., Nava-Diaz, C., & Miller, S. A. (2004). Identification and management of Colletotrichum acutatum on immature bell peppers. Plant Disease, 88, 1198–1204.CrossRefGoogle Scholar
  12. Lin, S. W., Gniffke, P. A., & Wang, T. C. (2007). Inheritance of resistance to pepper anthracnose by Colletotrichum acutatum. Acta Horticulture (ISHS), 760, 329–334.CrossRefGoogle Scholar
  13. Mahasuk, P., Khumpeng, N., Wasee, S., Taylor, P. W. J., & Mongkolporn, O. (2009a). Inheritance of resistance to anthracnose (cColletotrichum capsici) at seedling and fruiting stages in chilli pepper (Capsicum spp.). Plant Breeding, 128, 701–706.CrossRefGoogle Scholar
  14. Mahasuk, P., Taylor, P. W. J., & Mongkolporn, O. (2009b). Identification of two new genes conferring resistance to Colletotrichum acutatum in Capsicum baccatum. Phytopathology, 99(9), 1100–1104.CrossRefPubMedGoogle Scholar
  15. Mahasuk, P., Chinthaisong, J., & Mongkolporn, O. (2013). Differential resistances to anthracnose in Capsicum baccatum as responding to two Colletotrichum pathotypes and inoculation methods. Breeding Science, 63, 333–338.PubMedCentralCrossRefPubMedGoogle Scholar
  16. Montri, P. P., Taylor, P. W. J., & Mongkolporn, O. (2009). Pathotypes of Colletotrichum capsici the causal agent of chili anthracnose in Thailand. Plant Disease, 93, 17–20.CrossRefGoogle Scholar
  17. Pakdeevaraporn, P., Wasee, S., Taylor, P. W. J., & Mongkolporn, O. (2005). Inheritance of resistance to anthracnose caused Colletotrichum capsici in Capsicum. Plant Breeding, 124, 206–208.CrossRefGoogle Scholar
  18. Park, K. S., & Kim, C. H. (1992). Identification, distribution, and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean Journal Plant Pathology, 8, 61–69.Google Scholar
  19. Park, K. S., Kim, S. H., Park, H. G., & Yoon, J. B. (2009). Capsicum germplasm resistance to pepper anthracnose differentially interacts with Colletotrichum isolates. Horticulture Environment and Biotechnology, 50(1), 17–23.Google Scholar
  20. Poonpolgul, S., & Kumphai, S. (2007). Chili pepper anthracnose in Thailand. First International Symposium. on Chili Anthracnose, Seoul National University, Korea, 23.Google Scholar
  21. Silva, S. A., Rodrigues, R., Goncalves, L. S. A., Sudre, C. P., Bento, C. S., Carmo, M. G. F., & Medeiros, A. M. (2014). Resistance in Capsicum spp. to anthracnose affected by different stages of fruit development during pre- and post harvest. Tropical Plant Pathology, 39(4), 335–341.CrossRefGoogle Scholar
  22. Sun, C., Mao, S. L., Zhang, Z. H., Palloix, A., Wang, L. H., & Zhang, B. X. (2015). Resistances to anthracnose (Colletotrichum acutatum) of Capsicum mature green and ripe fruit are controlled by a major dominant cluster of QTLs on chromosome P5. Scientia Horticulturae, 181, 81–88.CrossRefGoogle Scholar
  23. Tanksley, S. D., & Nelson, J. C. (1996). Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theoretical and Applied Genetics, 92, 191–203.CrossRefPubMedGoogle Scholar
  24. Taylor, P. W. J., & Ford, R. (2007). Diagnostics, genic diversity and pathogenic variation of Ascochyta blight of cool season food and feed legumes. European Journal of Plant Pathology, 119, 127–133.CrossRefGoogle Scholar
  25. Temiyakul, P., Taylor, P. W. J., & Mongkolporn, O. (2010). Development of a double-inoculation method to assess resistance to anthracnose in trispecies Capsicum hybrid. Journal of Phytopathology, 158, 561–565.CrossRefGoogle Scholar
  26. Than, P. P., Jeewon, R., Hyde, K. D., Pongsupasamit, S., Mongkolporn, O., & Taylor, P. W. J. (2008a). Characterization and pathogenicity of Collecotrichum species associated with anthracnose disease on chili (Capsicum ssp.) in Thailand. Plant Pathology, 57(3), 562–572.CrossRefGoogle Scholar
  27. Than, P. P., Prihastuti, H., Phoulivong, S., Taylor, P. W. J., & Hyde, K. D. (2008b). Chili anthracnose disease caused by Colletotrichum species. Journal Zhejiang University Science Biomedicine and Biotechnology, 9(10), 764–778.CrossRefGoogle Scholar
  28. Voorrips, R. E., Finkers, R., Sanjaya, L., & Groenwold, R. (2004). QTL mapping of anthracnose (Colletotrichum spp.) resistance in a cross between Capsicum annuum and C. chinense. Theoretical and Applied Genetics, 109, 1275–1282.CrossRefPubMedGoogle Scholar
  29. Wang, Y. W. (2011). Development of Sequence characterized amplified region (SCAR) markers associated with pepper anthracnose (Colletotrichum acutatum) resistance. Master Thesis, Department of Agronomy, National Chiayi University, Taiwan.Google Scholar
  30. Yi, G., Lee, J. M., Lee, S., Choi, D., & Kim, B. D. (2006). Exploitation of pepper EST-SSRs and an SSR-based linkage map. Theoretical and Applied Genetics, 114, 113–130.CrossRefPubMedGoogle Scholar
  31. Yoon, J. B., Yang, D. C., Lee, W. P., Ahn, S. Y., & Park, H. G. (2004). Genetic resources resistant to anthracnose in the genus Capsicum. Journal of the Korean Society for Horticultural Science, 45, 318–323.Google Scholar
  32. Yoon, J. B., Yang, D. C., Do, J. W., & Park, H. G. (2006). Overcoming two post-fertilization genetic barriers in interspecific hybridization between Capsicum annuum and C. baccatum for introgression of anthracnose resistance. Breeding Science, 56, 31–38.CrossRefGoogle Scholar
  33. Yu, S. M., Ramkumar, G., & Lee, Y. H. (2013). Light quality influences the virulence and physiological responses of Colletotrichum acutatum causing anthracnose in pepper plants. Journal of Applied Microbiology, 115(2), 509–516.CrossRefPubMedGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2015

Authors and Affiliations

  • Patcharaporn Suwor
    • 1
  • Petcharat Thummabenjapone
    • 1
  • Jirawat Sanitchon
    • 1
  • Sanjeet Kumar
    • 2
  • Suchila Techawongstien
    • 1
  1. 1.Department of Plant Science and Agricultural Resources, Faculty of AgricultureKhon Kaen UniversityKhon KaenThailand
  2. 2.AVRDC – The World Vegetable CenterShanhua, TainanTaiwan

Personalised recommendations