Identification and characterization of Fusarium spp. associated with root rots of field pea in North Dakota
- 753 Downloads
Root rots are a major concern in field pea production in North Dakota. However, it is unclear which pathogens are involved in causing these diseases. This report brings together findings from surveys conducted over four years (2004, 2005, 2008, and 2009). The 2004 and 2005 surveys were mainly aimed at establishing the importance of pea root rot in North Dakota and providing an indication of the most prevalent root rot pathogens. The 2008 and 2009 surveys involved thorough evaluation of root rot incidence and severity, and included isolations and characterization of Fusarium species associated with the root rots. Greater mean root rot incidence and severity were observed in 2009 compared to three previous years. Fusarium species were the most frequently isolated fungal species from infected pea roots, of which F. oxysporum (66.7 and 94.7 % of the fields) and F. avenaceum (71.8 and 89.5 % of the fields) were most commonly isolated in 2008 and 2009, respectively. Pathogenicity tests showed that all nine Fusarium species isolated from symptomatic roots were capable of causing root rot of pea, and isolates of F. avenaceum were the most virulent at causing root rot. Significant differences in virulence were observed among F. avenaceum isolates. The prevalence of F. avenaceum on roots of field peas, and the ability of isolates of this species to cause severe root rot, emphasizes the possibility of this pathogen to emerge as a potential risk under the current cropping practices for pulse crops in North Dakota, and potentially in other regions with similar growing conditions.
KeywordsFusarium Root rot Field pea Fusarium avenaceum Virulence Pathogenicity
The F. solani f. sp. solani isolates used as positive control in these studies were kindly provided by Dr. Lyndon Porter, USDA-ARS, Prosser, WA. The 2004–05 survey was funded by the United States Department of Agriculture-Cooperative State Research, Education, and Extension Service (USDA-CSREES) Cool Season Food Legume Research Program. The 2008 and 2009 survey was funded by the Northern Pulse Growers Association.
- Basu, P. K., Brown, N. J., Crête, R., Gourley, C. O., Johnston, H. W., Pepin, H. S., & Seaman, W. L. (1976). Yield loss conversion factors for Fusarium root rot of pea. Canadian Plant Disease Survey, 56, 25–32.Google Scholar
- Booth, C. (1971). The Genus Fusarium. Commonw. Mycol. Inst., Kew, England.Google Scholar
- Hwang, S. F., & Chang, K. F. (1989). Incidence and severity of root rot disease complex of field pea in northeastern Alberta in 1988. Canadian Plant Disease Survey, 69, 139–141.Google Scholar
- Jones, F. R. (1923). Stem and root rot of peas in the United States caused by species of Fusarium. Journal of Agricultural Research, 26, 459–477.Google Scholar
- Kraft, J. M., & Pfleger, F. L. (2001). Compendium of Pea Diseases and Pests (2nd ed.). St. Paul: The American Phytopathological Society.Google Scholar
- Yli-Mattila, T., Paavanen-Huhtala, S., Bulat, S. A., Alekhina, I. A., & Nirenberg, H. I. (2002). Molecular, morphological and phylogenetic analysis of the Fusarium avenaceum/ F. arthrosporiodes/ F. tricinctum species complex- a polyphasic approach. Mycological Research, 106, 655–669.CrossRefGoogle Scholar