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Pathogenicity testing of lesser-known fungal trunk pathogens associated with wood decay of almond trees


Cross infection of multiple hosts by fungal trunk pathogens has been reported to occur worldwide. It supports the hypothesis that infected hosts adjacent to vineyards may serve as sources of inoculum for these pathogens. Among the alternative hosts, different studies have shown that almond represents a rich catch-crop for many fungal trunk pathogens. The hyphomycetes Collophora hispanica, Phaeoacremonium (Pm.) amygdalinum, Pm. iranianum and Pleurostomophora richardsiae have recently been isolated from wood samples with internal necroses and brown to black vascular streaking of almond trees showing symptoms of decline in Spain, although their status as pathogens was so far unknown. We inoculated almond trees cv. Ferragnes with these fungal species in greenhouse experiments to determine their pathogenic potential. All of them infected, colonised and produced lesions statistically different from the control, Pl. richardsiae being the most aggressive. Therefore, Co. hispanica, Pm. amygdalinum, Pm. iranianum and Pl. richardsiae should be considered as trunk pathogens of almond trees.

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  • Arzanlou, M., Narmani, A., Khodaei, S., & Moshari, S. (2014). Pome and stone fruit trees as possible reservoir hosts for Phaeoacremonium spp., the causal agents of grapevine esca disease, in Iran. Archives of Phytopathology and Plant Protection, 47, 717–727.

    Article  Google Scholar 

  • Carlucci, A., Lops, F., Marchi, G., Mugnai, L., & Surico, G. (2013a). Has Xylella fastidiosa “chosen” olive trees to establish in the Mediterranean basin? Phytopathologia Mediterranea, 52, 541–544.

    Google Scholar 

  • Carlucci, A., Raimondo, M. L., Cibelli, F., Phillips, J. L., & Lops, F. (2013b). Pleurostomophora richardsiae, Neofusicoccum parvum and Phaeoacremonium aleophilum associated with a decline of olives in southern Italy. Phytopathologia Mediterranea, 52, 517–527.

    Google Scholar 

  • Carlucci, A., Lops, F., Cibelli, F., & Raimondo, M. L. (2014). Phaeoacremonium species associated with olive wilt and decline in southern Italy. European Journal of Plant Pathology, 141, 717–729.

    Article  Google Scholar 

  • Carter, M. V. (1982). Additional hosts of Eutypa armeniacae in Australia. Australasian Plant Pathology, 11, 46–48.

    Article  Google Scholar 

  • Cloete, M., Fourie, P. H., Damm, U., Crous, P. W., & Mostert, L. (2011). Fungi associated with die-back symptoms of apple and pear trees, a possible inoculum source of grapevine trunk disease pathogens. Phytopathologia Mediterranea, 50, S176–S190.

    Google Scholar 

  • Damm, U., Mostert, L., Crous, P. W., & Fourie, P. H. (2008). Novel Phaeoacremonium species associated with necrotic wood of Prunus trees. Persoonia, 20, 87–102.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Damm, U., Fourie, P. H., & Crous, P. W. (2010). Coniochaeta (Lecythophora)), Collophora gen. nov. and Phaeomoniella species associated with wood necroses of Prunus trees. Persoonia, 24, 60–80.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ellis, M. B., & Ellis, J. P. (1997). Microfungi on land plants: An identification handbook. The Richmond Publishing Co., Ltd.

  • English, H., Davis, J. R., & deVay, J. E. (1966). Dothiorella canker, a new disease of almond trees in California. Phytopathology, 56, 146.

    Google Scholar 

  • Essakhi, S., Mugnai, L., Crous, P. W., Groenewald, J. Z., & Surico, G. (2008). Molecular and phenotypic characterization of novel Phaeoacremonium species associated with Petri disease and esca of grapevine. Persoonia, 21, 119–134.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gramaje, D., Armengol, J., Colino, M., Santiago, R., Moralejo, E., Olmo, D., Luque, J., & Mostert, L. (2009a). First report of Phaeoacremonium inflatipes, P. iranianum and P. sicilianum causing Petri disease of grapevine in Spain. Plant Disease, 93, 964.

    Article  Google Scholar 

  • Gramaje, D., Armengol, J., Mohammadi, H., Banihashemi, Z., & Mostert, L. (2009b). Novel Phaeoacremonium species associated with Petri disease and esca of grapevines in Iran and Spain. Mycologia, 101, 920–929.

    Article  PubMed  Google Scholar 

  • Gramaje, D., Agustí-Brisach, C., Pérez-Sierra, A., Moralejo, E., Olmo, D., Mostert, L., Damm, U., & Armengol, J. (2012). Fungal trunk pathogens associated with wood decay of almond trees on Mallorca (Spain). Persoonia, 28, 1–13.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gramaje, D., León, M., Pérez-Sierra, A., Burgess, T., & Armengol, J. (2014a). New Phaeoacremonium species isolated from sandalwood trees in western Australia. IMA Fungus, 5, 67–77.

    Article  PubMed Central  PubMed  Google Scholar 

  • Gramaje, D., León, M., Santana, M., Crous, P. W., & Armengol, J. (2014b). Multilocus ISSR markers reveal two major genetic groups in Spanish and South African populations of the grapevine fungal pathogen Cadophora luteo-olivacea. PLoS ONE, 9(10), e110417.

    Article  PubMed Central  PubMed  Google Scholar 

  • Gramaje, D., Mostert, L., Groenewald, J. Z., & Crous, P. W. (2015). Phaeoacremonium: from esca disease to phaeohyphomycosis. Fungal Biology doi:10.1016/j.funbio.2015.06.004.

  • Halleen, F., Crous, P. W., & Petrini, O. (2003). Fungi associated with healthy grapevine cuttings in nurseries, with special reference to pathogens involved in the decline of young vines. Australasian Plant Pathology, 32, 47–52.

  • Halleen, F., Mostert, L., & Crous, P. W. (2007). Pathogenicity testing of lesser-known vascular fungi of grapevines. Australasian Plant Pathology, 36, 277–285.

    Article  Google Scholar 

  • Inderbitzin, P., Bostock, R. M., Trouillas, F. P., & Michailides, T. J. (2010). A six locus phylogeny reveals high species diversity in Botryosphaeriaceae from California almond. Mycologia, 102, 1350–1368.

    Article  CAS  PubMed  Google Scholar 

  • Mostert, L., Groenewald, J. Z., Summerbell, R. C., Gams, W., & Crous, P. W. (2006). Taxonomy and pathology of Togninia (Diaporthales) and its Phaeoacremonium anamorphs. Studies in Mycology, 54, 1–115.

    Article  Google Scholar 

  • Raimondo, M. L., Lops, F., & Carlucci, A. (2014). Phaeoacremonium italicum sp nov., a new species associated with esca of grapevine in southern Italy. Mycologia, 106, 1119–1126.

    Article  PubMed  Google Scholar 

  • Rolshausen, P. E., Úrbez-Torres, J. R., Rooney-Latham, S., Eskalen, A., Smith, R. J., & Gubler, W. D. (2010). Evaluation of pruning wound susceptibility and protection against fungi associated with grapevine trunk diseases. American Journal of Enology and Viticulture, 61, 113–119.

    Google Scholar 

  • Úrbez-Torres, J. R., Peduto, F., Vossen, P. M., Krueger, W. H., & Gubler, W. D. (2013). Olive twig and branch dieback: etiology, incidence, and distribution in California. Plant Disease, 97, 231–244.

    Article  Google Scholar 

  • Úrbez-Torres, J. R., Haag, P., Bowen, P., & O’Gorman, D. T. (2014). Grapevine trunk diseases in British Columbia: incidence and characterization of the fungal pathogens associated with esca and Petri diseases of grapevine. Plant Disease, 98, 469–482.

    Article  Google Scholar 

  • Vijaykrishna, D., Mostert, L., Jeewon, R., Gams, W., Hyde, K. D., & Crous, P. W. (2004). Pleurostomophora, an anamorph of Pleurostoma (Calosphaeriales), a new anamorph genus morphologically similar to Phialophora. Studies in Mycology, 50, 387–395.

    Google Scholar 

  • White, C. L., Halleen, F., Fischer, M., & Mostert, L. (2011). Characterisation of the fungi associated with esca diseased grapevines in South Africa. Phytopathologia Mediterranea, 50, S204–S223.

    Google Scholar 

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We thank A. Ramón for assistance with inoculations and disease evaluation.

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Correspondence to David Gramaje.

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Olmo, D., Armengol, J., León, M. et al. Pathogenicity testing of lesser-known fungal trunk pathogens associated with wood decay of almond trees. Eur J Plant Pathol 143, 607–611 (2015).

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  • Almond dieback
  • Collophora
  • Phaeoacremonium
  • Pleurostomophora
  • Prunus dulcis