Transcriptome profiling of in vitro lines propagated from corm bud tip of elephant foot yam (Amorphophallus paeoniifolius) approves complete potyviruses elimination
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The most characterised Dasheen mosaic virus and many other unreported putative viruses are involved in the mixed viral mosaic infection of elephant foot yam (Amorphophallus paeoniifolius). The in vitro propagation of corm bud tips for virus free plantlet production was carried out in three different culture phases consisting of callusing, shoot regeneration and rooting. A 100 % survival rate was recorded on hardening in sand: soil: coir pith (1:1:1) mixture. A total of 84 % of regenerated plantlets were found to be virus free on indexing of 21 in vitro lines with species specific/genus specific serological and molecular diagnostic techniques. Transcriptome sequencing was carried out for two randomly selected in vitro plants and a mosaic infected field sample. Not any of the known potyviruses were traced in the transcriptome profiles of supposed virus free plants thus confirming the complete potyviruses elimination. Disease symptoms or re-occurrence was not observed in the hardened virus-free lines of the plant.
KeywordsCorm bud Callus Elephant foot yam RT-PCR Transcriptome profiling Virus free lines
We acknowledge the Director, ICAR-Central Tuber Crops Research Institute for providing all the facilities to carry out this work. We express our sincere gratitude to Dr. Stephan Winter (DSMZ, Germany) for his supportive suggestions and for kindly providing the antisera for ELISA. The Senior Research Fellowship granted by Council for Scientific and Industrial Research, India to the first author is thankfully acknowledged.
Conflict of interest
The authors declare that they have no competing interests.
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