Acibenzolar-S-Methyl (ASM) is a functional analog of salicylic acid (SA) that activates local and systemic acquired resistance (SAR) responses in plants against a wide variety of pathogens. Iris yellow spot virus (IYSV) is an economically important tospovirus of onion that causes severe economic losses to both bulb and seed crops. IYSV resistant onion cultivars are not available to date and there are limited control options. To explore the possibility of utilizing SAR as a control option, we first used two different hosts of IYSV, Datura stramonium and Nicotiana benthamiana, to study the ability of ASM in triggering SAR against IYSV infection. Quantitative descriptors based on both symptom expression and relative levels of IYSV nucleoprotein and viral small RNA were developed and used to determine the SAR in ASM- and buffer-treated plants. A significant reduction in virus levels in ASM-treated plants was noticed by ELISA and PCR. The level of SAR response was also assessed by measuring the IYSV lesion size and number on the inoculated leaves of ASM-treated plants. ASM-treated plants showed reduced symptoms compared to buffer-treated plants. This study could be useful in potentially developing novel SAR-based options for virus management.
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We thank Mrs. Nomatter Chingandu for her help with real-time PCR. Tripathi was supported by a research assistantship from the WSU Graduate Program in Molecular Plant Sciences. PPNS No. 0672, Department of Plant Pathology, College of Agricultural, Human and Natural Resource Sciences, Agricultural Research Center, Project # WNPO 0545, Washington State University, Pullman, WA 99164-6430, USA. This research was funded in part by the USDA-NIFA Specialty Crops Research Initiative SREP grant (2008-51180-04875).
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Tripathi, D., Pappu, H.R. Evaluation of acibenzolar-S-methyl-induced resistance against iris yellow spot tospovirus . Eur J Plant Pathol 142, 855–864 (2015). https://doi.org/10.1007/s10658-015-0657-0
- Chemical inducers
- Disease management
- Disease resistance
- Systemic acquired resistance