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Efficacy of a plant extract of Macleaya cordata (Willd.) R. Br. against downy mildew of cucumber (Pseudoperonospora cubensis)

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Abstract

Phytopathogenic oomycetes are present worldwide and cause large losses in important crops. Because of strict regulation of copper based fungicides there is a need of alternative plant protecting agents. On this background the efficacy of a plant extract of Macleaya cordata (Willd.) R. Br. was assessed against downy mildew of cucumber (Pseudoperonospora cubensis). The plant extract of M. cordata was produced by using an optimized process including the upgrading of the active ingredients (sanguinarine and chelerythrine). At very low concentrations of 40–50 μg/ml the upgraded extract gave rise to a 90 % reduction of the infestation of cucumber with P. cubensis in bioassays after protective treatment. The plant extract reduced the release and the survival of zoospores of the in vitro cultivable oomycete Phytophthora infestans. The growth of mycelia of P. infestans was also inhibited. In an assay on leaf discs, released and encysted zoospores of P. cubensis could be found, but only few germ tubes were formed.

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Acknowledgments

The authors thank Phytobiotics GmbH (Walluferstraße 10a, 65343 Eltville) for providing the plant extract from Macleaya cordata and the financial support. Karin Bald and Mona von Eitzen-Ritter are thanked for their technical assistance. The authors are grateful to Prof. Dr. Craig Martin, University of Kansas, USA, and to Dr. Andreas Leclerque, Geisenheim University, Germany, for critical reading of the manuscript.

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  1. Julius Kühn-Institute, Institute for Biological Control, Heinrichstraße 243, 64287, Darmstadt, Germany

    Christina Schuster & Annegret Schmitt

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  1. Christina Schuster
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  2. Annegret Schmitt
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Correspondence to Christina Schuster.

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Schuster, C., Schmitt, A. Efficacy of a plant extract of Macleaya cordata (Willd.) R. Br. against downy mildew of cucumber (Pseudoperonospora cubensis). Eur J Plant Pathol 142, 567–575 (2015). https://doi.org/10.1007/s10658-015-0635-6

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