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The fire blight resistance QTL of Malus fusca (Mfu10) is affected but not broken down by the highly virulent Canadian Erwinia amylovora strain E2002A

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Abstract

Recently, Mfu10 - the major QTL for resistance to fire blight was reported on linkage group 10 of Malus fusca which could explain about 66 % of the phenotypic variation in a population artificially inoculated with Erwinia amylovora strain Ea222_JKI. In the meantime, it had been reported that the resistance of the fire blight QTL of Malus × robusta 5 (Mr5), which could explain 80 % of phenotypic variation is strain specific and is completely overcome by the highly virulent strain Ea3049 originating from Canada (E2002A). In contrast to Mr5, the M. fusca donor of Mfu10 is only very slightly affected by this highly virulent strain. In this short communication, we prove that this particular strain is not able to overcome the resistance of M. fusca but affects Mfu10. The same F1 progenies, derived from a cross between the resistant M. fusca and the susceptible apple cultivar, Idared, used to detect Mfu10, were used for phenotyping with Ea3049. Although the mean shoot necrosis of all progenies was 62.4 %, marker-phenotype association determined by Kruskal-Wallis analysis showed that markers on LG10 correlate significantly to resistance levels with SSR markers FR481A and FRM4 having the highest K values of 37.1 and 36.7, respectively. Interval mapping and multiple QTL mapping (MQM) performed with MAP-QTL®, showed that Mfu10 could still be detected on the same position on LG10, but only explaining 41.2 % of phenotypic variation. The implications of these results are discussed.

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References

  • Broggini, G. A. L., Wöhner, T., Fahrentrapp, J., Kost, T. D., Flachowsky, H., Peil, A., Hanke, M.-V., Richter, K., Patocchi, A., & Gessler, C. (2014). Engineering fire blight resistance into the apple cultivar ‘Gala’ using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5. Plant Biotechnology Journal 13, 728–733.

  • Calenge, F., Drouet, D., Denance, C., Van de Weg, W. E., Brisset, M. N., Paulin, J. P., & Durel, C. E. (2005). Identification of a major QTL together with several minor additive or espistatic QTLs for resistance to fire blight in apple in two related progenies. Theoretical and Applied Genetics, 111, 128–135.

    Article  CAS  PubMed  Google Scholar 

  • Durel, C. E., Denance, C., & Brisset, M. N. (2009). Two distinct major QTL for resistance to fire blight co-localize on linkage group 12 in apple genotypes ‘Evereste’ and Malus floribunda clone 821. Genome, 52, 139–147.

    Article  CAS  PubMed  Google Scholar 

  • Emeriewen, O., Richter, K., Kilian, A., Zini, E., Hanke, M.-V., Malnoy, M., & Peil, A. (2014). Identification of a major quantitative trait locus for resistance to fire blight in the wild apple species Malus fusca. Molecular Breeding 34, 407–419.

  • Fahrentrapp, J., Broggini, G. A. L., Kellerhals, M., Peil, A., Richter, K., Zini, E., & Gessler, C. (2013). A candidate gene for fire blight resistance in Malus × robusta 5 is coding for a CC-NBS-LRR. Tree Genetics and Genomes, 9, 237–251.

    Article  Google Scholar 

  • Khan, M. A., Duffy, B., Gessler, C., & Patocchi, A. (2006). QTL mapping of fire blight resistance. Mol Breeding, 17, 299–306.

    Article  Google Scholar 

  • Le Roux, P. M. F., Khan, M. A., Broggini, G. A. L., Duffy, B., Gessler, C., & Patocchi, A. (2010). Mapping of quantitative trait loci for fire blight resistance in the apple cultivars ‘Florina’ and ‘Nova Easygro’. Genome, 53, 710–722.

    Article  PubMed  Google Scholar 

  • Norelli, J. N., & Aldwinckle, H. S. (1986). Differential susceptibility of Malus spp. cultivars Robusta 5, Novole, and Ottawa 523 to Erwinia amylovora. Plant Disease, 70, 1017–1019.

    Article  Google Scholar 

  • Peil, A., Garcia-Libreros, T., Richter, K., Trognitz, F. C., Trognitz, B., Hanke, M. V., & Flachowsky, H. (2007). Strong evidence for a fire blight resistance gene of Malus robusta located on linkage group 3. Plant Breeding, 126, 270–475.

    Article  Google Scholar 

  • Peil, A., Hanke, M. V., Flachowsky, H., Richter, K., Garcia-Libreros, T., Celton, J. M., Gardiner, S., Horner, M., & Bus, V. (2008). Confirmation of the fire blight QTL of Malus × robusta 5 on linkage group 3. Acta Horticulturae, 793, 297–303.

    CAS  Google Scholar 

  • Peil, A., Flachowsky, H., Hanke, M.-V., Richter, K., & Rode, J. (2011). Inoculation of Malus × robusta 5 progeny with a strain breaking resistance to fire blight reveals a minor QTL on LG5. Acta Horticulturae, 986, 357–62.

    Google Scholar 

  • Van Ooijen, J.W. (2004). MapQTL® 5 Software for the mapping of quantitative trait loci in experimental populations. Plant Research International, Wageningen, the Netherlands.

  • Vogt, I., Wöhner, T., Richter, K., Flachowsky, H., Sundin, G. W., Wensing, A., Savory, E. A., Geider, K., Day, B., Hanke, M. V., & Peil, A. (2013). Gene-for-gene relationship in the host-pathogen system Malus × robusta 5-Erwinia amylovora. New Phytologist, 197, 1262–1275.

    Article  CAS  PubMed  Google Scholar 

  • Wöhner, T. W., Flachowsky, H., Richter, K., Garcia-Libreros, T., Trognitz, F., Hanke, M.-V., & Peil, A. (2014). QTL mapping of fire blight resistance in Malus × robusta 5 after inoculation with different strains of Erwinia amylovora. Mol Breeding 34, 217–230.

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Acknowledgments

This research was funded by the Genomics and Molecular Physiology of Fruits (GMPF) programme, (Reference number 0004915/AG/gt), Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (Trentino), Italy.

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Correspondence to Andreas Peil.

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Emeriewen, O.F., Richter, K., Hanke, MV. et al. The fire blight resistance QTL of Malus fusca (Mfu10) is affected but not broken down by the highly virulent Canadian Erwinia amylovora strain E2002A. Eur J Plant Pathol 141, 631–635 (2015). https://doi.org/10.1007/s10658-014-0565-8

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