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Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria

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Abstract

We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007–2010 from fields located in central Bohemia and central and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57 %) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens.

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Acknowledgments

This study received financial support from Grant Agency of the Czech Republic (GA CR) grant 521/08/H042, from the Ministry of Agriculture of the Czech Republic, grant QH71229, from the Grant Agency of the Academy of Sciences of the Czech Republic, grant AV0Z50510513 and from Czech Ministry of Education, Youth and Sports, grant No. CZ.1.07/2.3.00/30.0049

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Authors and Affiliations

  1. Faculty of Agriculture, University of South Bohemia, Studentska 13, , 370 05, Ceske Budejovice, Czech Republic

    Pavel Beran, Ivan Mraz & Andrea Bohata

  2. Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic

    Ivan Mraz

  3. Laboratory of Diagnostics and Epidemiology of Microorganisms, Plant Medicine Division, Crop Research Institute, Drnovska 507, 161 06, Prague 6, Czech Republic

    Blanka Kokoskova

Authors
  1. Pavel Beran
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  2. Ivan Mraz
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  3. Blanka Kokoskova
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  4. Andrea Bohata
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Correspondence to Pavel Beran.

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Beran, P., Mraz, I., Kokoskova, B. et al. Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria . Eur J Plant Pathol 141, 617–621 (2015). https://doi.org/10.1007/s10658-014-0554-y

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  • DOI: https://doi.org/10.1007/s10658-014-0554-y

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